IKZF3 Recombinant Rabbit mAb - #BF4385

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Product: IKZF3 Recombinant Rabbit mAb
Catalog: BF4385
Description: Rabbit monoclonal antibody to IKZF3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 58 kDa(Observed); 58kD(Calculated).
Uniprot: Q9UKT9

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:2000-1:20000, IHC 1:50-1:100, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse, Rat
Clonality:
Monoclonal [ReFirm23835]
Specificity:
IKZF3 Recombinant Rabbit mAb detects endogenous levels of IKZF3.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AIO; Aiolos; IKAROS family zinc finger 3 (Aiolos); IKAROS family zinc finger 3; Ikaros family zinc finger protein 3; IKZF 3; IKZF3; IKZF3_HUMAN; zinc finger DNA binding protein Aiolos; Zinc finger protein Aiolos; Zinc finger protein subfamily 1A 3 (Aiolos); Zinc finger protein subfamily 1A 3; Zinc finger protein subfamily 1A, member 3; ZNFN1A3;

Immunogens

Immunogen:

A synthetic peptide from human IKZF3

Uniprot:

Q9UKT9(IKZF3_HUMAN) >>Visit HPA database.

Gene(ID):
IKZF3(22806)
Expression:
Q9UKT9 IKZF3_HUMAN:

Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.

Description:
This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros,Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers,Ikaros,Aiolos heterodimers,and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described,as well as some non-protein coding variants.
Sequence:
MEDIQTNAELKSTQEQSVPAESAAVLNDYSLTKSHEMENVDSGEGPANEDEDIGDDSMKVKDEYSERDENVLKSEPMGNAEEPEIPYSYSREYNEYENIKLERHVVSFDSSRPTSGKMNCDVCGLSCISFNVLMVHKRSHTGERPFQCNQCGASFTQKGNLLRHIKLHTGEKPFKCHLCNYACQRRDALTGHLRTHSVEKPYKCEFCGRSYKQRSSLEEHKERCRTFLQSTDPGDTASAEARHIKAEMGSERALVLDRLASNVAKRKSSMPQKFIGEKRHCFDVNYNSSYMYEKESELIQTRMMDQAINNAISYLGAEALRPLVQTPPAPTSEMVPVISSMYPIALTRAEMSNGAPQELEKKSIHLPEKSVPSERGLSPNNSGHDSTDTDSNHEERQNHIYQQNHMVLSRARNGMPLLKEVPRSYELLKPPPICPRDSVKVINKEGEVMDVYRCDHCRVLFLDYVMFTIHMGCHGFRDPFECNMCGYRSHDRYEFSSHIARGEHRALLK

Research Backgrounds

Function:

Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.

PTMs:

Phosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells.

Subcellular Location:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.

Family&Domains:

Belongs to the Ikaros C2H2-type zinc-finger protein family.

Restrictive clause

 

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