Podocin Recombinant Rabbit mAb - #BF4389

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Product: Podocin Recombinant Rabbit mAb
Catalog: BF4389
Description: Rabbit monoclonal antibody to Podocin
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 42 kDa(Observed); 42kD(Calculated).
Uniprot: Q9NP85

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MSDS
Data Sheet
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Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000, IHC 1:200-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse, Rat
Clonality:
Monoclonal [ReFirm23839]
Specificity:
Podocin Recombinant Rabbit mAb detects endogenous levels of Podocin.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Nephrosis 2, idiopathic, steroid resistant (podocin); nephrosis 2, idiopathic, steroid resistant; NPHS2; NPHS2 gene; PDCN; PODO_HUMAN; Podocin; SRN1;

Immunogens

Immunogen:

A synthetic peptide from human Podocin

Uniprot:

Q9NP85(PODO_HUMAN) >>Visit HPA database.

Gene(ID):
NPHS2(7827)
Expression:
Q9NP85 PODO_HUMAN:

Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.

Description:
This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants.
Sequence:
MERRARSSSRESRGRGGRTPHKENKRAKAERSGGGRGRQEAGPEPSGSGRAGTPGEPRAPAATVVDVDEVRGSGEEGTEVVALLESERPEEGTKSSGLGACEWLLVLISLLFIIMTFPFSIWFCVKVVQEYERVIIFRLGHLLPGRAKGPGLFFFLPCLDTYHKVDLRLQTLEIPFHEIVTKDMFIMEIDAICYYRMENASLLLSSLAHVSKAVQFLVQTTMKRLLAHRSLTEILLERKSIAQDAKVALDSVTCIWGIKVERIEIKDVRLPAGLQHSLAVEAEAQRQAKVRMIAAEAEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTEKPSTVVLPLPFDLLNCLSSPSNRTQGSLPFPSPSKPVEPLNPKKKDSPML

Research Backgrounds

Function:

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.

PTMs:

Isoform 2 is glycosylated.

Subcellular Location:

Cell membrane>Peripheral membrane protein.

Endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.

Family&Domains:

Belongs to the band 7/mec-2 family.

Restrictive clause

 

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