TLE2 Antibody - #DF3239

Product: TLE2 Antibody
Catalog: DF3239
Description: Rabbit polyclonal antibody to TLE2
Application: WB IF/ICC
Reactivity: Human
Mol.Wt.: 110 KD; 80kD(Calculated).
Uniprot: Q04725
RRID: AB_2835619

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
TLE2 Antibody detects endogenous levels of total TLE2.
RRID:
AB_2835619
Cite Format: Affinity Biosciences Cat# DF3239, RRID:AB_2835619.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

enhancer of split groucho 2; enhancer of split groucho 2; Enhancer of split groucho-like protein 2; ESG; ESG2; FLJ41188; GRG2; Groucho2; KIAA4188; MGC124808; mKIAA4188; Tle2; Tle2 protein; TLE2_HUMAN; transducin like enhancer of split 2; Transducin like enhancer of split 2, homolog of Drosophila E(sp1); Transducin like enhancer protein 2; Transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila); Transducin-like enhancer of split 2, homolog of Drosophila E(spl); Transducin-like enhancer protein 2;

Immunogens

Immunogen:
Uniprot:

Q04725(TLE2_HUMAN) >>Visit HPA database.

Gene(ID):
TLE2(7089)
Expression:
Q04725 TLE2_HUMAN:

In all tissues examined, mostly in heart, brain, and muscle.

Sequence:
MYPQGRHPTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQLQPLSHHAPPVPLTPRPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSPPESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARRDLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDSVALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVSSSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGGKGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

PTMs - Q04725 As Substrate

Site PTM Type Enzyme
Ubiquitination
K29 Ubiquitination
K53 Ubiquitination
K82 Ubiquitination
S182 Phosphorylation
S193 Phosphorylation
S195 Phosphorylation
S199 Phosphorylation
S271 Phosphorylation
S281 Phosphorylation
K287 Ubiquitination
K477 Ubiquitination
K481 Ubiquitination
K491 Ubiquitination
K510 Ubiquitination
K540 Ubiquitination
Y665 Phosphorylation
S672 Phosphorylation
S676 Phosphorylation
S681 Phosphorylation
K691 Acetylation
K691 Ubiquitination
K710 Ubiquitination
S712 Phosphorylation
K725 Ubiquitination
Y726 Phosphorylation
T729 Phosphorylation
S731 Phosphorylation
K734 Ubiquitination

Research Backgrounds

Function:

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).

PTMs:

Ubiquitinated by XIAP/BIRC4.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

In all tissues examined, mostly in heart, brain, and muscle.

Subunit Structure:

Homooligomer and heterooligomer with other family members. Binds LEF1, TCF7, TCF7L1, TCF7L2, UTY, HES1 and HES5.

Family&Domains:

WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.

Belongs to the WD repeat Groucho/TLE family.

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