Product: CSTF1 Antibody
Catalog: DF3241
Description: Rabbit polyclonal antibody to CSTF1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 48 KD; 48kD(Calculated).
Uniprot: Q05048
RRID: AB_2835621

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
CSTF1 Antibody detects endogenous levels of total CSTF1.
RRID:
AB_2835621
Cite Format: Affinity Biosciences Cat# DF3241, RRID:AB_2835621.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CF 1 50 kDa subunit; CF-1 50 kDa subunit; Cleavage stimulation factor 50 kDa subunit; Cleavage stimulation factor 50kDa subunit; Cleavage stimulation factor; Cleavage stimulation factor subunit 1; Cleavage stimulation factor, 3 prime pre RNA, subunit 1, 50 kD; cleavage stimulation factor, 3' pre RNA, subunit 1, 50kD; cleavage stimulation factor, 3' pre RNA, subunit 1, 50kDa; CstF 50; CSTF 50 kDa subunit; CstF-50; CSTF1; CSTF1_HUMAN; CstFp50; OTTHUMP00000031332;

Immunogens

Immunogen:

A synthesized peptide derived from human CSTF1, corresponding to a region within N-terminal amino acids.

Uniprot:
Gene(ID):
Sequence:
MYRTKVGLKDRQQLYKLIISQLLYDGYISIANGLINEIKPQSVCAPSEQLLHLIKLGMENDDTAVQYAIGRSDTVAPGTGIDLEFDADVQTMSPEASEYETCYVTSHKGPCRVATYSRDGQLIATGSADASIKILDTERMLAKSAMPIEVMMNETAQQNMENHPVIRTLYDHVDEVTCLAFHPTEQILASGSRDYTLKLFDYSKPSAKRAFKYIQEAEMLRSISFHPSGDFILVGTQHPTLRLYDINTFQCFVSCNPQDQHTDAICSVNYNSSANMYVTGSKDGCIKLWDGVSNRCITTFEKAHDGAEVCSAIFSKNSKYILSSGKDSVAKLWEISTGRTLVRYTGAGLSGRQVHRTQAVFNHTEDYVLLPDERTISLCCWDSRTAERRNLLSLGHNNIVRCIVHSPTNPGFMTCSDDFRARFWYRRSTTD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
91
Xenopus
80
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.

PTMs:

The N-terminus is blocked.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

N-terminus mediates homodimerization.

Research Fields

· Genetic Information Processing > Translation > mRNA surveillance pathway.

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