PRMT1 Recombinant Rabbit mAb - #BF4473

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Product: PRMT1 Recombinant Rabbit mAb
Catalog: BF4473
Description: Rabbit monoclonal antibody to PRMT1
Application: WB IHC
Reactivity: Human, Mouse
Mol.Wt.: 43 kDa(Observed); 42kD(Calculated).
Uniprot: Q99873

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IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:5000, IHC 1:100-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse
Clonality:
Monoclonal [ReFirm23923]
Specificity:
PRMT1 Recombinant Rabbit mAb detects endogenous levels of PRMT1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ANM 1; ANM1; ANM1_HUMAN; HCP 1; HCP1; Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2; Heterogeneous nuclear ribonucleoproteins methyltransferase like 2; Heterogeneous nuclear ribonucleoproteins methyltransferase like2; Histone-arginine N-methyltransferase PRMT1; HMT 2; HMT1 (hnRNP methyltransferase S. cerevisiae) like 2; HMT1 hnRNP methyltransferase; HMT1 hnRNP methyltransferase like 2 (S. cerevisiae); HMT1 hnRNP methyltransferase like 2; HMT2; HRMT1 L2; HRMT1L 2; HRMT1L2; Human mRNA for suppressor for yeast mutant; Human mRNA for suppressor for yeast mutant complete cds; Interferon receptor 1 bound protein 4; Interferon receptor 1 bound protein4; Interferon receptor 1-bound protein 4; Interferon receptor 1bound protein 4; IR1 B4; IR1B 4; IR1B4; Mrmt 1; Mrmt1; PRMT 1; PRMT1; Protein arginine methyltransferase 1; Protein arginine N methyltransferase 1; Protein arginine N methyltransferase1; Protein arginine N-methyltransferase 1; R1B4; S. cerevisiae like 2;

Immunogens

Immunogen:

A synthetic peptide from human PRMT1

Uniprot:

Q99873(ANM1_HUMAN) >>Visit HPA database.

Gene(ID):
PRMT1(3276)
Expression:
Q99873 ANM1_HUMAN:

Widely expressed (PubMed:11097842). Expressed strongly in colorectal cancer cells (at protein level) (PubMed:28040436). Expressed strongly in colorectal cancer tissues compared to wild-type colon samples (at protein level) (PubMed:28040436). Expressed strongly in colorectal cancer tissues compared to wild-type colon samples (PubMed:28040436).

Description:
This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes,whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene,and a pseudogene of this gene is located on the long arm of chromosome 5.
Sequence:
MAAAEAANCIMENFVATLANGMSLQPPLEEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLYARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR

Research Backgrounds

Function:

Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4 and SERBP1. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity. Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner.

PTMs:

Polyubiquitinated at Lys-145 by the SCF(FBXL17) complex, leading to its subsequent degradation. Ubiquitination is regulated by acetylation at Lys-228 and Lys-233.

Acetylation at Lys-228 and Lys-233 regulates ubiquitination by the SCF(FBXL17) complex. Acetylated at Lys-233 by p300/EP300. Deacetylated at Lys-228 and Lys-233 by SIRT1.

Subcellular Location:

Nucleus. Nucleus>Nucleoplasm. Cytoplasm. Cytoplasm>Cytosol.
Note: Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Expressed strongly in colorectal cancer cells (at protein level). Expressed strongly in colorectal cancer tissues compared to wild-type colon samples (at protein level). Expressed strongly in colorectal cancer tissues compared to wild-type colon samples.

Family&Domains:

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.

Research Fields

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

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