Product: NCBP1 Antibody
Catalog: DF3246
Description: Rabbit polyclonal antibody to NCBP1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 80 KD; 92kD(Calculated).
Uniprot: Q09161
RRID: AB_2835626

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 100ul $280 In stock
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Product Info

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(85%), Dog(100%), Chicken(83%), Xenopus(91%)
NCBP1 Antibody detects endogenous levels of total NCBP1.
Cite Format: Affinity Biosciences Cat# DF3246, RRID:AB_2835626.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


80 kDa nuclear cap-binding protein; CBP80; MGC2087; NCBP 80 kDa subunit; NCBP; ncbp1; NCBP1_HUMAN; nuclear cap binding protein subunit 1; nuclear cap binding protein subunit 1, 80kDa; Nuclear cap-binding protein subunit 1; Sto1;





Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q09161 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation P23443 (RPS6KB1)
T21 Phosphorylation P23443 (RPS6KB1)
S22 Phosphorylation P23443 (RPS6KB1)
T27 Phosphorylation
S33 Phosphorylation
C36 S-Nitrosylation
K37 Ubiquitination
K41 Ubiquitination
C73 S-Nitrosylation
K117 Ubiquitination
Y126 Phosphorylation
S175 Phosphorylation
R194 Methylation
S201 Phosphorylation
K204 Acetylation
K204 Methylation
K204 Ubiquitination
S306 Phosphorylation
K330 Ubiquitination
S386 Phosphorylation
T395 Phosphorylation
K455 Ubiquitination
C477 S-Nitrosylation
C483 S-Nitrosylation
K511 Ubiquitination
T513 Phosphorylation
K522 Ubiquitination
K574 Acetylation
K583 Ubiquitination
T611 Phosphorylation
T633 Phosphorylation
S643 Phosphorylation
T644 Phosphorylation
K654 Ubiquitination
K657 Ubiquitination
S674 Phosphorylation
K684 Acetylation
K684 Ubiquitination
K698 Acetylation
K698 Ubiquitination
K707 Acetylation
S734 Phosphorylation
T737 Phosphorylation

Research Backgrounds


Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Associates with NCBP3 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export and is particularly important in cellular stress situations such as virus infections. The conventional CBC with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus whereas the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role only in mRNA export. NCBP1/CBP80 is required for cell growth and viability.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the nuclear cap-binding complex (CBC), a heterodimer composed of NCBP1/CBP80 and NCBP2/CBP20 that interacts with m7GpppG-capped RNA. Found in a U snRNA export complex containing PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20, RAN, XPO1 and m7G-capped RNA. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with PHAX/RNUXA, SRRT/ARS2, EIF4G2, IGF2BP1, HNRNPF, HNRNPH1, KIAA0427/CTIF, PARN, DROSHA, UPF1 and ALYREF/THOC4. May interact with EIF4G1; the interaction is however controversial since it is reported byand but is not observed by. The large PER complex involved in the repression of transcriptional termination is composed of at least PER2, CDK9, DDX5, DHX9, NCBP1/CBP80 and POLR2A. Component of an alternative nuclear cap-binding complex (CBC) composed of NCBP1/CBP80 and NCBP3. Interacts with METTL3. Interacts with ZFC3H1 in a RNase-insensitive manner. Interacts with MTREX.


Belongs to the NCBP1 family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Genetic Information Processing > Transcription > Spliceosome.

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