Product: FOXC1/2 Antibody
Catalog: DF3252
Description: Rabbit polyclonal antibody to FOXC1/2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Xenopus
Mol.Wt.: 57 KD; 57kD,54kD(Calculated).
Uniprot: Q12948 | Q99958
RRID: AB_2835632

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Xenopus(100%)
FOXC1/2 Antibody detects endogenous levels of total FOXC1/2.
Cite Format: Affinity Biosciences Cat# DF3252, RRID:AB_2835632.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


ARA; FKH L7; FKHL 7; FKHL7; Forkhead (Drosophila) like 7; Forkhead box C1; Forkhead box protein C1; Forkhead drosophila homolog like 7; Forkhead like 7; Forkhead related activator 3; Forkhead related protein FKHL7; Forkhead related transcription factor 3; Forkhead-related protein FKHL7; Forkhead-related transcription factor 3; FOX C1; FOXC 1; Foxc1; FOXC1_HUMAN; FREAC 3; FREAC-3; FREAC3; IGDA; IHG 1; IHG1; IRID 1; IRID1; Iridogoniodysgenesis type 1; Myeloid factor delta; Drosphilia Forkhead Homolog Like 14; FKHL 14; FKHL14; Forkhead Box C2; Forkhead box protein C2; Forkhead related protein FKHL14; Forkhead-related protein FKHL14; FOX C2; Foxc2; FOXC2_HUMAN; LD; Mesenchyme fork head protein 1; Mesenchyme Forkhead 1; MFH 1; MFH 1 protein; MFH-1 protein; MFH1; Transcription factor FKH 14; Transcription factor FKH-14;



Expressed in keratinocytes of epidermis and hair follicle (PubMed:27907090). Expressed strongly in microvascular invasion (MVI) formation, basal-like breast cancer (BLBC) and hepatocellular tumors (PubMed:20406990, PubMed:22991501). Expressed in breast cancers (at protein level) (PubMed:26565916). Expressed in hematopoietic cells (PubMed:8499623).





Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12948/Q99958 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
S12 Phosphorylation
R62 Methylation
T68 Phosphorylation P24941 (CDK2)
S125 Phosphorylation
K138 Sumoylation
Y159 Phosphorylation
K227 Sumoylation
T228 Phosphorylation
T232 Phosphorylation
S235 Phosphorylation
S241 Phosphorylation
S248 Phosphorylation
S259 Phosphorylation
S272 Phosphorylation
S320 Phosphorylation
S323 Phosphorylation
S329 Phosphorylation
S330 Phosphorylation
S335 Phosphorylation
S339 Phosphorylation
S521 Phosphorylation
S543 Phosphorylation
K552 Ubiquitination
Site PTM Type Enzyme
S36 Phosphorylation
S119 Phosphorylation
K132 Sumoylation
Y153 Phosphorylation
T197 Phosphorylation
K214 Sumoylation
S215 Phosphorylation
S219 Phosphorylation
T226 Phosphorylation
T230 Phosphorylation
S232 Phosphorylation
S235 Phosphorylation
S240 Phosphorylation
T247 Phosphorylation
S251 Phosphorylation
S255 Phosphorylation
T280 Phosphorylation
S281 Phosphorylation
S288 Phosphorylation
S367 Phosphorylation
T484 Phosphorylation
Y494 Phosphorylation
Y496 Phosphorylation
T499 Phosphorylation
Y501 Phosphorylation

Research Backgrounds


DNA-binding transcriptional factor that plays a role in a broad range of cellular and developmental processes such as eye, bones, cardiovascular, kidney and skin development. Acts either as a transcriptional activator or repressor. Binds to the consensus binding site 5'-[G/C][A/T]AAA[T/C]AA[A/C]-3' in promoter of target genes. Upon DNA-binding, promotes DNA bending. Acts as a transcriptional coactivator. Stimulates Indian hedgehog (Ihh)-induced target gene expression mediated by the transcription factor GLI2, and hence regulates endochondral ossification (By similarity). Acts also as a transcriptional coregulator by increasing DNA-binding capacity of GLI2 in breast cancer cells. Regulates FOXO1 through binding to a conserved element, 5'-GTAAACAAA-3' in its promoter region, implicating FOXC1 as an important regulator of cell viability and resistance to oxidative stress in the eye. Cooperates with transcription factor FOXC2 in regulating expression of genes that maintain podocyte integrity (By similarity). Promotes cell growth inhibition by stopping the cell cycle in the G1 phase through TGFB1-mediated signals. Involved in epithelial-mesenchymal transition (EMT) induction by increasing cell proliferation, migration and invasion. Involved in chemokine CXCL12-induced endothelial cell migration through the control of CXCR4 expression (By similarity). Plays a role in the gene regulatory network essential for epidermal keratinocyte terminal differentiation. Essential developmental transcriptional factor required for mesoderm-derived tissues, such as the somites, skin, bone and cartilage. Positively regulates CXCL12 and stem cell factor expression in bone marrow mesenchymal progenitor cells, and hence plays a role in the development and maintenance of mesenchymal niches for haematopoietic stem and progenitor cells (HSPC). Plays a role in corneal transparency by preventing both blood vessel and lymphatic vessel growth during embryonic development in a VEGF-dependent manner. Involved in chemokine CXCL12-induced endothelial cell migration through the control of CXCR4 expression (By similarity). May function as a tumor suppressor.


Phosphorylated. Phosphorylated on Ser-272 in response to epidermal growth factor (EGF) in a ERK1/2 MAPK-dependent signaling pathway; phosphorylation contributes to its protein stability and transcriptional activity.

Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP2.

Ubiquitinated, leading to its proteasomal degradation.

Subcellular Location:

Note: Colocalizes with PITX2 isoform 3 in the nucleus at subnuclear chromatine regions (PubMed:16449236). Colocalizes with CBX5 to a heterochromatin-rich region of the nucleus (PubMed:15684392). Colocalizes with GLI2 in the nucleus (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in keratinocytes of epidermis and hair follicle. Expressed strongly in microvascular invasion (MVI) formation, basal-like breast cancer (BLBC) and hepatocellular tumors. Expressed in breast cancers (at protein level). Expressed in hematopoietic cells.

Subunit Structure:

Monomer. Interacts with C1QBP. Interacts (via N-terminus) with GLI2 (via C-terminal internal region); this interaction is direct and increases GLI2 DNA-binding and transcriptional activity through a smoothened (SMO)-independent Hedgehog (Hh) signaling pathway. Interacts (via C-terminus domain) with PITX2 isoform 3 (via homeobox domain). Interacts with FLNA and PBX1.


Transcriptional activator. Might be involved in the formation of special mesenchymal tissues.


Phosphorylation regulates FOXC2 transcriptional activity by promoting its recruitment to chromatin.

Subcellular Location:


Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location


1). Silencing FOXC1 inhibits growth and migration of human oral squamous cell carcinoma cells. Experimental and Therapeutic Medicine, 2018 (PubMed: 30233683) [IF=2.7]

Application: IHC    Species: human    Sample: OSCC tissues and adjacent normal tissues

Figure 1. |Upregulation of FOXC1 expression in OSCC tissues. (A) IHC staining of FOXC1 expression in OSCC tissues and adjacent normal tissues. Scale bar, 100 µm.

2). The expression of AGGF1, FOXC2, and E-cadherin in esophageal carcinoma and their clinical significance. Medicine, 2020 (PubMed: 32925786) [IF=1.6]

Application: IHC    Species: human    Sample: cancer cells

Figure 1. |Immunostaining for AGGF1 (angiogenic factor with G-patch and FHA domain 1), FOXC2 (forkhead box C2), and E-cad (E-cadherin) in esophageal squamous cell carcinoma and control tissue. ; E, Positive FOXC2 in the cytoplasm of cancer cells (400 magnification)

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