ENTPD5 Recombinant Rabbit mAb - #BF4560

Copy Info
Product: ENTPD5 Recombinant Rabbit mAb
Catalog: BF4560
Description: Rabbit monoclonal antibody to ENTPD5
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 48 kDa(Observed); 48kD(Calculated).
Uniprot: O75356

View similar products>>

Out of stock
Control Products
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:5000, IHC 1:100-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse, Rat
Clonality:
Monoclonal [ReFirm24010]
Specificity:
ENTPD5 Recombinant Rabbit mAb detects endogenous levels of ENTPD5.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD39 like 4; AI196558; AI987697; CD39 antigen like 4; CD39 antigen-like 4; CD39 like 4; CD39L4; Ectonucleoside triphosphate diphosphohydrolase 5; ENTP5_HUMAN; Entpd5; ER UDPase; ER-UDPase; GDPase ENTPD5; Guanosine-diphosphatase ENTPD5; MGC163357; MGC163359; mNTPase; NTPDase 5; Nucleoside diphosphatase; PCPH; Pcph proto oncogene protein; Proto oncogene CPH; UDPase ENTPD5; Uridine-diphosphatase ENTPD5;

Immunogens

Immunogen:

A synthetic peptide from human ENTPD5

Uniprot:

O75356(ENTP5_HUMAN) >>Visit HPA database.

Gene(ID):
ENTPD5(957)
Expression:
O75356 ENTP5_HUMAN:

Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues.

Description:
The protein encoded by this gene is similar to E-type nucleotidases (NTPases),ecto-ATPase,apyrases. NTPases,such as CD39,mediate catabolism of extracellular nucleotides. ENTPD5 contains 4 apyrase-conserved regions which is characteristic of NTPases.
Sequence:
MATSWGTVFFMLVVSCVCSAVSHRNQQTWFEGIFLSSMCPINVSASTLYGIMFDAGSTGTRIHVYTFVQKMPGQLPILEGEVFDSVKPGLSAFVDQPKQGAETVQGLLEVAKDSIPRSHWKKTPVVLKATAGLRLLPEHKAKALLFEVKEIFRKSPFLVPKGSVSIMDGSDEGILAWVTVNFLTGQLHGHRQETVGTLDLGGASTQITFLPQFEKTLEQTPRGYLTSFEMFNSTYKLYTHSYLGFGLKAARLATLGALETEGTDGHTFRSACLPRWLEAEWIFGGVKYQYGGNQEGEVGFEPCYAEVLRVVRGKLHQPEEVQRGSFYAFSYYYDRAVDTDMIDYEKGGILKVEDFERKAREVCDNLENFTSGSPFLCMDLSYITALLKDGFGFADSTVLQLTKKVNNIETGWALGATFHLLQSLGISH

Research Backgrounds

Function:

Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. The nucleotide hydrolyzing preference is GDP > IDP > UDP, but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.

PTMs:

N-glycosylated; high-mannose type (By similarity). Glycosylation is not essential for enzymatic activity.

Subcellular Location:

Endoplasmic reticulum. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues.

Family&Domains:

Belongs to the GDA1/CD39 NTPase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.