CAF1B Antibody - #DF3255
| Product: | CAF1B Antibody |
| Catalog: | DF3255 |
| Description: | Rabbit polyclonal antibody to CAF1B |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 61 KD; 61kD(Calculated). |
| Uniprot: | Q13112 |
| RRID: | AB_2835635 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3255, RRID:AB_2835635.
Fold/Unfold
CAF 1 subunit B; CAF 1A; CAF I 60 kDa subunit; CAF IP60; CAF-1; CAF-1 subunit B; CAF-I 60 kDa subunit; CAF-I p60; CAF1; CAF1A; CAF1B_HUMAN; CAF1P60; CHAF 1B; CHAF1B; Chromatin assembly factor 1 subunit B; Chromatin assembly factor I; Chromatin assembly factor I p60 subunit; Human chromatin assembly factor-I p60 subunit; M phase phosphoprotein 7; M-phase phosphoprotein 7; MPHOSPH7; MPP 7; MPP7; p60 subunit;
Immunogens
- Q13112 CAF1B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKVITCEIAWHNKEPVYSLDFQHGTAGRIHRLASAGVDTNVRIWKVEKGPDGKAIVEFLSNLARHTKAVNVVRFSPTGEILASGGDDAVILLWKVNDNKEPEQIAFQDEDEAQLNKENWTVVKTLRGHLEDVYDICWATDGNLMASASVDNTAIIWDVSKGQKISIFNEHKSYVQGVTWDPLGQYVATLSCDRVLRVYSIQKKRVAFNVSKMLSGIGAEGEARSYRMFHDDSMKSFFRRLSFTPDGSLLLTPAGCVESGENVMNTTYVFSRKNLKRPIAHLPCPGKATLAVRCCPVYFELRPVVETGVELMSLPYRLVFAVASEDSVLLYDTQQSFPFGYVSNIHYHTLSDISWSSDGAFLAISSTDGYCSFVTFEKDELGIPLKEKPVLNMRTPDTAKKTKSQTHRGSSPGPRPVEGTPASRTQDPSSPGTTPPQARQAPAPTVIRDPPSITPAVKSPLPGPSEEKTLQPSSQNTKAHPSRRVTLNTLQAWSKTTPRRINLTPLKTDTPPSSVPTSVISTPSTEEIQSETPGDAQGSPPELKRPRLDENKGGTESLDP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13112 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K13 | Ubiquitination | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| T66 | Phosphorylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| T397 | Phosphorylation | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| R407 | Methylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| T419 | Phosphorylation | Uniprot | |
| S422 | Phosphorylation | Uniprot | |
| T424 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| T433 | Phosphorylation | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| K477 | Ubiquitination | Uniprot | |
| T485 | Phosphorylation | Uniprot | |
| K494 | Acetylation | Uniprot | |
| K494 | Ubiquitination | Uniprot | |
| T496 | Phosphorylation | Uniprot | |
| T503 | Phosphorylation | Uniprot | |
| T509 | Phosphorylation | Uniprot | |
| S512 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| T524 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| T531 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| T554 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | Uniprot |
Research Backgrounds
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
Nucleus. Cytoplasm.
Note: DNA replication foci. Cytoplasmic in M phase.
Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. In G2 and S phase also monomeric CHAF1B is detected.
Belongs to the WD repeat HIR1 family.
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