MeCP2 Recombinant Rabbit mAb - #BF4601

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Product: MeCP2 Recombinant Rabbit mAb
Catalog: BF4601
Description: Rabbit monoclonal antibody to MeCP2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 75 kDa(Observed); 52kD(Calculated).
Uniprot: P51608

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000, IHC: 1:500-1:2000, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse, Rat
Clonality:
Monoclonal [ReFirm24051]
Specificity:
MeCP2 Recombinant Rabbit mAb detects endogenous levels of MeCP2.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AUTSX 3; AUTSX3; DKFZp686A24160; Mbd 5; Mbd5; MECP 2; MeCP 2 protein; MeCP-2 protein; Mecp2; MECP2_HUMAN; Methyl CpG binding protein 2 (Rett syndrome); Methyl CpG binding protein 2; Methyl-CpG-binding protein 2; MRX 16; MRX 79; MRX16; MRX79; MRXS 13; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP 10; WBP10;

Immunogens

Immunogen:

A synthetic peptide from human MeCP2

Uniprot:

P51608(MECP2_HUMAN) >>Visit HPA database.

Gene(ID):
MECP2(4204)
Expression:
P51608 MECP2_HUMAN:

Present in all adult somatic tissues tested.

Description:
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2,MBD1,MBD2,MBD3,and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins,with the exception of MBD3,is capable of binding specifically to methylated DNA. MECP2,MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members,MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells,but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome,a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms.
Sequence:
MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS

Research Backgrounds

Function:

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).

PTMs:

Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.

Subcellular Location:

Nucleus.
Note: Colocalized with methyl-CpG in the genome. Colocalized with TBL1X to the heterochromatin foci.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Present in all adult somatic tissues tested.

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