Product: DYR1A Antibody
Catalog: DF3270
Description: Rabbit polyclonal antibody to DYR1A
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 90 KD; 86kD(Calculated).
Uniprot: Q13627
RRID: AB_2835638

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(90%), Chicken(80%)
DYR1A Antibody detects endogenous levels of total DYR1A.
Cite Format: Affinity Biosciences Cat# DF3270, RRID:AB_2835638.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


;Dual specificity tyrosine phosphorylation regulated kinase; Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A; Dual specificity tyrosine phosphorylation regulated kinase 1; Dual specificity tyrosine phosphorylation regulated kinase 1A; Dual specificity tyrosine-phosphorylation-regulated kinase 1A; Dual specificity YAK 1 related kinase; Dual specificity YAK1 related kinase; Dual specificity YAK1-related kinase; DYR1A_HUMAN; DYRK 1; DYRK 1A; DYRK; DYRK1; Dyrk1a; DYRKA; hMNB; HP 86; HP86; Minibrain (Drosophila) homolog; Minibrain homolog; Minibrain, Drosophila, homolog of; MNB; MNB protein kinase; Mnb protein kinase homolog hp86; MNB protein kinase, Serine/threonine-specific; MNB/DYRK protein kinase; MNBH; MRD7; OTTHUMP00000109090; OTTHUMP00000109091; OTTHUMP00000109094; OTTHUMP00000174799; Protein kinase minibrain homolog; Serine/threonine kinase MNB;



Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.




Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13627 As Substrate

Site PTM Type Enzyme
K11 Ubiquitination
S14 Phosphorylation
S22 Phosphorylation
S97 Phosphorylation
Y104 Phosphorylation Q13627 (DYRK1A)
K105 Acetylation
Y111 Phosphorylation Q13627 (DYRK1A)
Y112 Phosphorylation
S127 Phosphorylation
K134 Ubiquitination
Y136 Phosphorylation
Y140 Phosphorylation Q13627 (DYRK1A)
Y145 Phosphorylation
Y147 Phosphorylation
K150 Ubiquitination
Y159 Phosphorylation Q13627 (DYRK1A)
K167 Ubiquitination
Y177 Phosphorylation Q13627 (DYRK1A)
K304 Ubiquitination
S310 Phosphorylation Q13627 (DYRK1A)
Y319 Phosphorylation Q13627 (DYRK1A)
Y321 Phosphorylation Q13627 (DYRK1A)
S324 Phosphorylation
S329 Phosphorylation
T402 Phosphorylation Q13627 (DYRK1A)
Y449 Phosphorylation Q13627 (DYRK1A)
K453 Ubiquitination
T466 Phosphorylation
S529 Phosphorylation Q13627 (DYRK1A)
T532 Phosphorylation
S538 Phosphorylation
T543 Phosphorylation
T553 Phosphorylation
S555 Phosphorylation
Y705 Phosphorylation
S706 Phosphorylation
S748 Phosphorylation
S758 Phosphorylation
S762 Phosphorylation

PTMs - Q13627 As Enzyme

Substrate Site Source
O00401 (WASL) T199 Uniprot
O00401 (WASL) T205 Uniprot
O00401 (WASL) T262 Uniprot
O43524 (FOXO3) S330 Uniprot
O43597 (SPRY2) T75 Uniprot
O75533 (SF3B1) T273 Uniprot
O75533 (SF3B1) T434 Uniprot
P01106 (MYC) S62 Uniprot
P04637 (TP53) S15 Uniprot
P10636-8 (MAPT) S199 Uniprot
P10636-8 (MAPT) S202 Uniprot
P10636-8 (MAPT) T205 Uniprot
P10636-8 (MAPT) T212 Uniprot
P10636-8 (MAPT) T217 Uniprot
P10636 (MAPT) S369 Uniprot
P10636-8 (MAPT) S396 Uniprot
P10636-8 (MAPT) S404 Uniprot
P10636-8 (MAPT) S422 Uniprot
P10636 (MAPT) S519 Uniprot
P10636 (MAPT) T529 Uniprot
P10636 (MAPT) S721 Uniprot
P16220 (CREB1) S133 Uniprot
P24385 (CCND1) T286 Uniprot
P24928 (POLR2A) S1616 Uniprot
P24928 (POLR2A) S1619 Uniprot
P37840 (SNCA) S87 Uniprot
P46821 (MAP1B) S1400 Uniprot
P49418 (AMPH) S262 Uniprot
P49418 (AMPH) S272 Uniprot
P49418 (AMPH) S276 Uniprot
P49418 (AMPH) S285 Uniprot
P49418 (AMPH) S293 Uniprot
P49418 (AMPH) S295 Uniprot
P49418 (AMPH) T310 Uniprot
P49418 (AMPH) T312 Uniprot
P49768 (PSEN1) T354 Uniprot
P53805 (RCAN1) S167 Uniprot
P53805 (RCAN1) T247 Uniprot
P55211 (CASP9) T125 Uniprot
P61764 (STXBP1) T479 Uniprot
Q05193 (DNM1) S795 Uniprot
Q05193 (DNM1) S857 Uniprot
Q07955 (SRSF1) S227 Uniprot
Q07955 (SRSF1) S234 Uniprot
Q07955 (SRSF1) S238 Uniprot
Q12778 (FOXO1) S329 Uniprot
Q13627 (DYRK1A) Y104 Uniprot
Q13627 (DYRK1A) Y111 Uniprot
Q13627 (DYRK1A) Y140 Uniprot
Q13627 (DYRK1A) Y159 Uniprot
Q13627 (DYRK1A) Y177 Uniprot
Q13627 (DYRK1A) S310 Uniprot
Q13627 (DYRK1A) Y319 Uniprot
Q13627 (DYRK1A) Y321 Uniprot
Q13627 (DYRK1A) T402 Uniprot
Q13627 (DYRK1A) Y449 Uniprot
Q13627-1 (DYRK1A) S519 Uniprot
Q13627-2 (DYRK1A) S520 Uniprot
Q13627 (DYRK1A) S529 Uniprot
Q49AN0 (CRY2) S558 Uniprot
Q52LA3 (LIN52) S28 Uniprot
Q96EB6 (SIRT1) T530 Uniprot
Q96S94 (CCNL2) S330 Uniprot
Q96S94-1 (CCNL2) S338 Uniprot
Q96S94-1 (CCNL2) S369 Uniprot

Research Backgrounds


Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis (By similarity).


Autophosphorylated on numerous tyrosine residues. Can also autophosphorylate on serine and threonine residues (in vitro).

Subcellular Location:

Nucleus. Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.

Subunit Structure:

Interacts RAD54L2/ARIP4 (By similarity). Interacts with CRY2 (By similarity). Interacts with RANBP9. Interacts with WDR68. Interacts with SRSF6. Interacts with SIRT1 (By similarity).

(Microbial infection) Interacts with human adenovirus 5 E1A protein.


The polyhistidine repeats act as targeting signals to nuclear speckles.

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.


1). MicroRNA-221-3p inhibits the inflammatory response of keratinocytes by regulating the DYRK1A/STAT3 signaling pathway to promote wound healing in diabetes. Communications biology, 2024 (PubMed: 38461326) [IF=5.9]

Application: WB    Species: Mouse    Sample:

Fig. 4 Effect of DYRK1A on HG-induced inflammatory and diabetes wound healing. a Venn diagram showing the number of potential target genes for miR-221-3p in three major miRNA target gene databases. b Venn diagram showing the number of the genes overlapping across the four indicated groups. c Sequence alignment of the wild-type (WT) 3′-untranslated region (3′-UTR) of DYRK1A containing putative miR-221-3p-binding sites and the miR-221-3p sequence. Mutated (mut) 3ʹ-UTR of DYRK1A without the putative miR-221-3p-binding site. d Relative luciferase activities of plasmids carrying WT or mutant DYRK1A 3ʹ-UTR in HEK293T cells co-transfected with miRNA mimic negative control (NC) or miR-221-3p mimic (n = 3) were determined using the Luciferase Dual Reporter gene assay detection system. e Representative image and summary data showing protein expression levels of DYRK1A. Protein band intensities were normalized to β-Actin (n = 3). Summary data showing relative mRNA levels of IL-1β (f), IL-6 (g), IL-8 (h) and TNF-α (i). ACTB was used as the internal reference (n = 3). Representative photomicrographs (j) and summary data (k) showing changes in the area of excisional wounds on days 0, 2, 4, 8, and 10 in diabetic mice injected with either negative control (DM-Ctrl), DYRK1A siRNA (DM-si) or both DYRK1A siRNA and miR-221-3p agomir (DM-si+mi). The smallest scale division of the ruler is 1 mm in (j). Data are presented as mean ± SEM, n = 5–7. *P 

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