ApoM Antibody - #BF0109

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Product: ApoM Antibody
Catalog: BF0109
Description: Mouse monoclonal antibody to ApoM
Application: WB IF/ICC ELISA
Reactivity: Human
Mol.Wt.: 21kDa; 21kD(Calculated).
Uniprot: O95445
RRID: AB_2833500

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFB1475]
Specificity:
ApoM antibody detects endogenous levels of total ApoM.
RRID:
AB_2833500
Cite Format: Affinity Biosciences Cat# BF0109, RRID:AB_2833500.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Apo M; Apo-M; Apolipoprotein M; ApoM; APOM_HUMAN; G3A; HSPC336; MGC22400; NG20; NG20 like protein; Protein G3a;

Immunogens

Immunogen:

Purified recombinant fragment of human ApoM expressed in E. Coli.

Uniprot:

O95445(APOM_HUMAN) >>Visit HPA database.

Gene(ID):
APOM(55937)
Expression:
O95445 APOM_HUMAN:

Plasma protein. Expressed in liver and kidney.

Description:
ApoM (apolipoprotein M, also designated G3a or NG20), with 188-amino acid protein(about 21kDa), is an apolipoprotein and member of the lipocalin protein family. The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles.
Sequence:
MFHQIWAALLYFYGIILNSIYQCPEHSQLTTLGVDGKEFPEVHLGQWYFIAGAAPTKEELATFDPVDNIVFNMAAGSAPMQLHLRATIRMKDGLCVPRKWIYHLTEGSTDLRTEGRPDMKTELFSSSCPGGIMLNETGQGYQRFLLYNRSPHPPEKCVEEFKSLTSCLDSKAFLLTPRNQEACELSNN

PTMs - O95445 As Substrate

Site PTM Type Enzyme
N135 N-Glycosylation

Research Backgrounds

Function:

Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.

Subcellular Location:

Secreted.
Note: Present in high density lipoprotein (HDL) and to a lesser extent in triglyceride-rich lipoproteins (TGRLP) and low density lipoproteins (LDL).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Plasma protein. Expressed in liver and kidney.

Subunit Structure:

Interacts with LRP2; LRP2 mediates APOM renal uptake and subsequent lysosomal degradation.

Family&Domains:

Belongs to the calycin superfamily. Lipocalin family. Highly divergent.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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