ZNF638 Antibody - #DF3280

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Product: ZNF638 Antibody
Catalog: DF3280
Description: Rabbit polyclonal antibody to ZNF638
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Dog
Mol.Wt.: 220 KD; 221kD(Calculated).
Uniprot: Q14966
RRID: AB_2835648

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(80%), Dog(80%)
Clonality:
Polyclonal
Specificity:
ZNF638 Antibody detects endogenous levels of total ZNF638.
RRID:
AB_2835648
Cite Format: Affinity Biosciences Cat# DF3280, RRID:AB_2835648.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CTCL tumor antigen se33 1; CTCL-associated antigen se33-1; Cutaneous T cell lymphoma associated antigen se33 1; Cutaneous T-cell lymphoma-associated antigen se33-1; DKFZp686P1231; MGC26130; MGC90196; NP220; NP220 nuclear protein; Nuclear protein 220; ZFML; Zfp638; Zinc finger matrin like; Zinc finger matrin like protein; Zinc finger matrin-like protein; Zinc finger protein 638; ZN638_HUMAN; ZNF 638; Znf638;

Immunogens

Immunogen:
Uniprot:

Q14966(ZN638_HUMAN) >>Visit HPA database.

Gene(ID):
ZNF638(27332)
Sequence:
MSRPRFNPRGDFPLQRPRAPNPSGMRPPGPFMRPGSMGLPRFYPAGRARGIPHRFAGHESYQNMGPQRMNVQVTQHRTDPRLTKEKLDFHEAQQKKGKPHGSRWDDEPHISASVAVKQSSVTQVTEQSPKVQSRYTKESASSILASFGLSNEDLEELSRYPDEQLTPENMPLILRDIRMRKMGRRLPNLPSQSRNKETLGSEAVSSNVIDYGHASKYGYTEDPLEVRIYDPEIPTDEVENEFQSQQNISASVPNPNVICNSMFPVEDVFRQMDFPGESSNNRSFFSVESGTKMSGLHISGGQSVLEPIKSVNQSINQTVSQTMSQSLIPPSMNQQPFSSELISSVSQQERIPHEPVINSSNVHVGSRGSKKNYQSQADIPIRSPFGIVKASWLPKFSHADAQKMKRLPTPSMMNDYYAASPRIFPHLCSLCNVECSHLKDWIQHQNTSTHIESCRQLRQQYPDWNPEILPSRRNEGNRKENETPRRRSHSPSPRRSRRSSSSHRFRRSRSPMHYMYRPRSRSPRICHRFISRYRSRSRSRSPYRIRNPFRGSPKCFRSVSPERMSRRSVRSSDRKKALEDVVQRSGHGTEFNKQKHLEAADKGHSPAQKPKTSSGTKPSVKPTSATKSDSNLGGHSIRCKSKNLEDDTLSECKQVSDKAVSLQRKLRKEQSLHYGSVLLITELPEDGCTEEDVRKLFQPFGKVNDVLIVPYRKEAYLEMEFKEAITAIMKYIETTPLTIKGKSVKICVPGKKKAQNKEVKKKTLESKKVSASTLKRDADASKAVEIVTSTSAAKTGQAKASVAKVNKSTGKSASSVKSVVTVAVKGNKASIKTAKSGGKKSLEAKKTGNVKNKDSNKPVTIPENSEIKTSIEVKATENCAKEAISDAALEATENEPLNKETEEMCVMLVSNLPNKGYSVEEVYDLAKPFGGLKDILILSSHKKAYIEINRKAAESMVKFYTCFPVLMDGNQLSISMAPENMNIKDEEAIFITLVKENDPEANIDTIYDRFVHLDNLPEDGLQCVLCVGLQFGKVDHHVFISNRNKAILQLDSPESAQSMYSFLKQNPQNIGDHMLTCSLSPKIDLPEVQIEHDPELEKESPGLKNSPIDESEVQTATDSPSVKPNELEEESTPSIQTETLVQQEEPCEEEAEKATCDSDFAVETLELETQGEEVKEEIPLVASASVSIEQFTENAEECALNQQMFNSDLEKKGAEIINPKTALLPSDSVFAEERNLKGILEESPSEAEDFISGITQTMVEAVAEVEKNETVSEILPSTCIVTLVPGIPTGDEKTVDKKNISEKKGNMDEKEEKEFNTKETRMDLQIGTEKAEKNEGRMDAEKVEKMAAMKEKPAENTLFKAYPNKGVGQANKPDETSKTSILAVSDVSSSKPSIKAVIVSSPKAKATVSKTENQKSFPKSVPRDQINAEKKLSAKEFGLLKPTSARSGLAESSSKFKPTQSSLTRGGSGRISALQGKLSKLDYRDITKQSQETEARPSIMKRDDSNNKTLAEQNTKNPKSTTGRSSKSKEEPLFPFNLDEFVTVDEVIEEVNPSQAKQNPLKGKRKETLKNVPFSELNLKKKKGKTSTPRGVEGELSFVTLDEIGEEEDAAAHLAQALVTVDEVIDEEELNMEEMVKNSNSLFTLDELIDQDDCISHSEPKDVTVLSVAEEQDLLKQERLVTVDEIGEVEELPLNESADITFATLNTKGNEGDTVRDSIGFISSQVPEDPSTLVTVDEIQDDSSDLHLVTLDEVTEEDEDSLADFNNLKEELNFVTVDEVGEEEDGDNDLKVELAQSKNDHPTDKKGNRKKRAVDTKKTKLESLSQVGPVNENVMEEDLKTMIERHLTAKTPTKRVRIGKTLPSEKAVVTEPAKGEEAFQMSEVDEESGLKDSEPERKRKKTEDSSSGKSVASDVPEELDFLVPKAGFFCPICSLFYSGEKAMTNHCKSTRHKQNTEKFMAKQRKEKEQNEAEERSSR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
80
Dog
80
Sheep
44
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14966 As Substrate

Site PTM Type Enzyme
R33 Methylation
S36 Phosphorylation
R41 Methylation
R47 Methylation
R49 Methylation
R54 Methylation
Y61 Phosphorylation
R68 Methylation
K86 Ubiquitination
T125 Phosphorylation
S128 Phosphorylation
K130 Ubiquitination
S193 Phosphorylation
K196 Ubiquitination
K216 Ubiquitination
S283 Phosphorylation
S289 Phosphorylation
S299 Phosphorylation
S303 Phosphorylation
S366 Phosphorylation
R367 Methylation
S369 Phosphorylation
K371 Ubiquitination
S375 Phosphorylation O14757 (CHEK1)
R382 Methylation
S383 Phosphorylation
T409 Phosphorylation
S420 Phosphorylation
S488 Phosphorylation
S490 Phosphorylation
S492 Phosphorylation
S499 Phosphorylation
S502 Phosphorylation
S508 Phosphorylation
S510 Phosphorylation
S520 Phosphorylation
S522 Phosphorylation
S541 Phosphorylation
Y543 Phosphorylation
S552 Phosphorylation
R557 Methylation
S558 Phosphorylation
S560 Phosphorylation
S568 Phosphorylation
S571 Phosphorylation
K576 Ubiquitination
S585 Phosphorylation
T589 Phosphorylation
K593 Ubiquitination
K602 Ubiquitination
S605 Phosphorylation
T612 Phosphorylation
S614 Phosphorylation
T616 Phosphorylation
T623 Phosphorylation
S624 Phosphorylation
T626 Phosphorylation
S628 Phosphorylation
S630 Phosphorylation
S636 Phosphorylation
S641 Phosphorylation
S650 Phosphorylation
K653 Ubiquitination
S656 Phosphorylation
K658 Ubiquitination
S671 Phosphorylation
Y716 Phosphorylation
Y731 Phosphorylation
T738 Phosphorylation
S770 Phosphorylation
T773 Phosphorylation
S791 Phosphorylation
T795 Phosphorylation
S801 Phosphorylation
S808 Phosphorylation
T809 Phosphorylation
S815 Phosphorylation
S818 Phosphorylation
K853 Acetylation
K868 Acetylation
S885 Phosphorylation
K927 Ubiquitination
K943 Ubiquitination
K951 Acetylation
K951 Ubiquitination
Y1060 Phosphorylation
K1098 Ubiquitination
S1100 Phosphorylation
S1106 Phosphorylation
S1119 Phosphorylation
S1121 Phosphorylation
S1158 Phosphorylation
T1328 Phosphorylation
K1330 Acetylation
T1376 Phosphorylation
S1377 Phosphorylation
K1391 Acetylation
K1391 Ubiquitination
S1400 Phosphorylation
S1401 Phosphorylation
S1409 Phosphorylation
K1430 Ubiquitination
K1441 Acetylation
K1455 Acetylation
S1462 Phosphorylation
R1465 Methylation
S1468 Phosphorylation
S1472 Phosphorylation
K1477 Acetylation
K1477 Ubiquitination
S1479 Phosphorylation
K1480 Ubiquitination
S1575 Phosphorylation
S1641 Phosphorylation
S1656 Phosphorylation
S1667 Phosphorylation
K1676 Ubiquitination
S1697 Phosphorylation
S1797 Phosphorylation
S1825 Phosphorylation
T1851 Phosphorylation
K1860 Acetylation
K1866 Acetylation
S1882 Phosphorylation
S1888 Phosphorylation
K1891 Acetylation
S1893 Phosphorylation
S1913 Phosphorylation
S1938 Phosphorylation
K1958 Acetylation

Research Backgrounds

Function:

Transcription factor that binds to cytidine clusters in double-stranded DNA. Plays a key role in the silencing of unintegrated retroviral DNA: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed. Mediates transcriptional repression of unintegrated viral DNA by specifically binding to the cytidine clusters of retroviral DNA and mediating the recruitment of chromatin silencers, such as the HUSH complex, SETDB1 and the histone deacetylases HDAC1 and HDAC4. Acts as an early regulator of adipogenesis by acting as a transcription cofactor of CEBPs (CEBPA, CEBPD and/or CEBPG), controlling the expression of PPARG and probably of other proadipogenic genes, such as SREBF1 (By similarity). May also regulate alternative splicing of target genes during adipogenesis (By similarity).

Subcellular Location:

Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with FHL2. Interacts with CEBPA, CEBPD and CEBPG (By similarity). Interacts with MPHOSPH8 and TASOR components of the HUSH complex; leading to recruitment of the HUSH complex. Interacts with SETDB1. Interacts with HDAC1. Interacts with HDAC4.

Family&Domains:

The matrin-type zinc finger domain is required for localization to nuclear speckles.

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