ZNF638 Antibody | Affinity Biosciences

Product:	ZNF638 Antibody
Catalog:	DF3280
Description:	Rabbit polyclonal antibody to ZNF638
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Pig, Dog
Mol.Wt.:	220 KD; 221kD(Calculated).
Uniprot:	Q14966
RRID:	AB_2835648

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(80%), Dog(80%)

Clonality:

Polyclonal

Specificity:

ZNF638 Antibody detects endogenous levels of total ZNF638.

RRID:

AB_2835648
Cite Format: Affinity Biosciences Cat# DF3280, RRID:AB_2835648.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CTCL tumor antigen se33 1; CTCL-associated antigen se33-1; Cutaneous T cell lymphoma associated antigen se33 1; Cutaneous T-cell lymphoma-associated antigen se33-1; DKFZp686P1231; MGC26130; MGC90196; NP220; NP220 nuclear protein; Nuclear protein 220; ZFML; Zfp638; Zinc finger matrin like; Zinc finger matrin like protein; Zinc finger matrin-like protein; Zinc finger protein 638; ZN638_HUMAN; ZNF 638; Znf638;

Immunogens

Immunogen:

Uniprot:

Q14966(ZN638_HUMAN) >>Visit HPA database.

Gene(ID):

ZNF638(27332)

Sequence:

Q14966 ZN638_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRPRFNPRGDFPLQRPRAPNPSGMRPPGPFMRPGSMGLPRFYPAGRARGIPHRFAGHESYQNMGPQRMNVQVTQHRTDPRLTKEKLDFHEAQQKKGKPHGSRWDDEPHISASVAVKQSSVTQVTEQSPKVQSRYTKESASSILASFGLSNEDLEELSRYPDEQLTPENMPLILRDIRMRKMGRRLPNLPSQSRNKETLGSEAVSSNVIDYGHASKYGYTEDPLEVRIYDPEIPTDEVENEFQSQQNISASVPNPNVICNSMFPVEDVFRQMDFPGESSNNRSFFSVESGTKMSGLHISGGQSVLEPIKSVNQSINQTVSQTMSQSLIPPSMNQQPFSSELISSVSQQERIPHEPVINSSNVHVGSRGSKKNYQSQADIPIRSPFGIVKASWLPKFSHADAQKMKRLPTPSMMNDYYAASPRIFPHLCSLCNVECSHLKDWIQHQNTSTHIESCRQLRQQYPDWNPEILPSRRNEGNRKENETPRRRSHSPSPRRSRRSSSSHRFRRSRSPMHYMYRPRSRSPRICHRFISRYRSRSRSRSPYRIRNPFRGSPKCFRSVSPERMSRRSVRSSDRKKALEDVVQRSGHGTEFNKQKHLEAADKGHSPAQKPKTSSGTKPSVKPTSATKSDSNLGGHSIRCKSKNLEDDTLSECKQVSDKAVSLQRKLRKEQSLHYGSVLLITELPEDGCTEEDVRKLFQPFGKVNDVLIVPYRKEAYLEMEFKEAITAIMKYIETTPLTIKGKSVKICVPGKKKAQNKEVKKKTLESKKVSASTLKRDADASKAVEIVTSTSAAKTGQAKASVAKVNKSTGKSASSVKSVVTVAVKGNKASIKTAKSGGKKSLEAKKTGNVKNKDSNKPVTIPENSEIKTSIEVKATENCAKEAISDAALEATENEPLNKETEEMCVMLVSNLPNKGYSVEEVYDLAKPFGGLKDILILSSHKKAYIEINRKAAESMVKFYTCFPVLMDGNQLSISMAPENMNIKDEEAIFITLVKENDPEANIDTIYDRFVHLDNLPEDGLQCVLCVGLQFGKVDHHVFISNRNKAILQLDSPESAQSMYSFLKQNPQNIGDHMLTCSLSPKIDLPEVQIEHDPELEKESPGLKNSPIDESEVQTATDSPSVKPNELEEESTPSIQTETLVQQEEPCEEEAEKATCDSDFAVETLELETQGEEVKEEIPLVASASVSIEQFTENAEECALNQQMFNSDLEKKGAEIINPKTALLPSDSVFAEERNLKGILEESPSEAEDFISGITQTMVEAVAEVEKNETVSEILPSTCIVTLVPGIPTGDEKTVDKKNISEKKGNMDEKEEKEFNTKETRMDLQIGTEKAEKNEGRMDAEKVEKMAAMKEKPAENTLFKAYPNKGVGQANKPDETSKTSILAVSDVSSSKPSIKAVIVSSPKAKATVSKTENQKSFPKSVPRDQINAEKKLSAKEFGLLKPTSARSGLAESSSKFKPTQSSLTRGGSGRISALQGKLSKLDYRDITKQSQETEARPSIMKRDDSNNKTLAEQNTKNPKSTTGRSSKSKEEPLFPFNLDEFVTVDEVIEEVNPSQAKQNPLKGKRKETLKNVPFSELNLKKKKGKTSTPRGVEGELSFVTLDEIGEEEDAAAHLAQALVTVDEVIDEEELNMEEMVKNSNSLFTLDELIDQDDCISHSEPKDVTVLSVAEEQDLLKQERLVTVDEIGEVEELPLNESADITFATLNTKGNEGDTVRDSIGFISSQVPEDPSTLVTVDEIQDDSSDLHLVTLDEVTEEDEDSLADFNNLKEELNFVTVDEVGEEEDGDNDLKVELAQSKNDHPTDKKGNRKKRAVDTKKTKLESLSQVGPVNENVMEEDLKTMIERHLTAKTPTKRVRIGKTLPSEKAVVTEPAKGEEAFQMSEVDEESGLKDSEPERKRKKTEDSSSGKSVASDVPEELDFLVPKAGFFCPICSLFYSGEKAMTNHCKSTRHKQNTEKFMAKQRKEKEQNEAEERSSR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Dog

Sheep

Horse

Bovine

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14966 As Substrate

Q14966

Site	PTM Type	Enzyme	Source
R33	Methylation		Uniprot
S36	Phosphorylation		Uniprot
R41	Methylation		Uniprot
R47	Methylation		Uniprot
R49	Methylation		Uniprot
R54	Methylation		Uniprot
Y61	Phosphorylation		Uniprot
R68	Methylation		Uniprot
K86	Ubiquitination		Uniprot
T125	Phosphorylation		Uniprot
S128	Phosphorylation		Uniprot
K130	Ubiquitination		Uniprot
S193	Phosphorylation		Uniprot
K196	Ubiquitination		Uniprot
K216	Ubiquitination		Uniprot
S283	Phosphorylation		Uniprot
S289	Phosphorylation		Uniprot
S299	Phosphorylation		Uniprot
S303	Phosphorylation		Uniprot
S366	Phosphorylation		Uniprot
R367	Methylation		Uniprot
S369	Phosphorylation		Uniprot
K371	Ubiquitination		Uniprot
S375	Phosphorylation	O14757 (CHEK1)	Uniprot
R382	Methylation		Uniprot
S383	Phosphorylation		Uniprot
T409	Phosphorylation		Uniprot
S420	Phosphorylation		Uniprot
S488	Phosphorylation		Uniprot
S490	Phosphorylation		Uniprot
S492	Phosphorylation		Uniprot
S499	Phosphorylation		Uniprot
S502	Phosphorylation		Uniprot
S508	Phosphorylation		Uniprot
S510	Phosphorylation		Uniprot
S520	Phosphorylation		Uniprot
S522	Phosphorylation		Uniprot
S541	Phosphorylation		Uniprot
Y543	Phosphorylation		Uniprot
S552	Phosphorylation		Uniprot
R557	Methylation		Uniprot
S558	Phosphorylation		Uniprot
S560	Phosphorylation		Uniprot
S568	Phosphorylation		Uniprot
S571	Phosphorylation		Uniprot
K576	Ubiquitination		Uniprot
S585	Phosphorylation		Uniprot
T589	Phosphorylation		Uniprot
K593	Ubiquitination		Uniprot
K602	Ubiquitination		Uniprot
S605	Phosphorylation		Uniprot
T612	Phosphorylation		Uniprot
S614	Phosphorylation		Uniprot
T616	Phosphorylation		Uniprot
T623	Phosphorylation		Uniprot
S624	Phosphorylation		Uniprot
T626	Phosphorylation		Uniprot
S628	Phosphorylation		Uniprot
S630	Phosphorylation		Uniprot
S636	Phosphorylation		Uniprot
S641	Phosphorylation		Uniprot
S650	Phosphorylation		Uniprot
K653	Ubiquitination		Uniprot
S656	Phosphorylation		Uniprot
K658	Ubiquitination		Uniprot
S671	Phosphorylation		Uniprot
Y716	Phosphorylation		Uniprot
Y731	Phosphorylation		Uniprot
T738	Phosphorylation		Uniprot
S770	Phosphorylation		Uniprot
T773	Phosphorylation		Uniprot
S791	Phosphorylation		Uniprot
T795	Phosphorylation		Uniprot
S801	Phosphorylation		Uniprot
S808	Phosphorylation		Uniprot
T809	Phosphorylation		Uniprot
S815	Phosphorylation		Uniprot
S818	Phosphorylation		Uniprot
K853	Acetylation		Uniprot
K868	Acetylation		Uniprot
S885	Phosphorylation		Uniprot
K927	Ubiquitination		Uniprot
K943	Ubiquitination		Uniprot
K951	Acetylation		Uniprot
K951	Ubiquitination		Uniprot
Y1060	Phosphorylation		Uniprot
K1098	Ubiquitination		Uniprot
S1100	Phosphorylation		Uniprot
S1106	Phosphorylation		Uniprot
S1119	Phosphorylation		Uniprot
S1121	Phosphorylation		Uniprot
S1158	Phosphorylation		Uniprot
T1328	Phosphorylation		Uniprot
K1330	Acetylation		Uniprot
T1376	Phosphorylation		Uniprot
S1377	Phosphorylation		Uniprot
K1391	Acetylation		Uniprot
K1391	Ubiquitination		Uniprot
S1400	Phosphorylation		Uniprot
S1401	Phosphorylation		Uniprot
S1409	Phosphorylation		Uniprot
K1430	Ubiquitination		Uniprot
K1441	Acetylation		Uniprot
K1455	Acetylation		Uniprot
S1462	Phosphorylation		Uniprot
R1465	Methylation		Uniprot
S1468	Phosphorylation		Uniprot
S1472	Phosphorylation		Uniprot
K1477	Acetylation		Uniprot
K1477	Ubiquitination		Uniprot
S1479	Phosphorylation		Uniprot
K1480	Ubiquitination		Uniprot
S1575	Phosphorylation		Uniprot
S1641	Phosphorylation		Uniprot
S1656	Phosphorylation		Uniprot
S1667	Phosphorylation		Uniprot
K1676	Ubiquitination		Uniprot
S1697	Phosphorylation		Uniprot
S1797	Phosphorylation		Uniprot
S1825	Phosphorylation		Uniprot
T1851	Phosphorylation		Uniprot
K1860	Acetylation		Uniprot
K1866	Acetylation		Uniprot
S1882	Phosphorylation		Uniprot
S1888	Phosphorylation		Uniprot
K1891	Acetylation		Uniprot
S1893	Phosphorylation		Uniprot
S1913	Phosphorylation		Uniprot
S1938	Phosphorylation		Uniprot
K1958	Acetylation		Uniprot

Research Backgrounds

Q14966_ZN638_HUMAN

Function:

Transcription factor that binds to cytidine clusters in double-stranded DNA. Plays a key role in the silencing of unintegrated retroviral DNA: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed. Mediates transcriptional repression of unintegrated viral DNA by specifically binding to the cytidine clusters of retroviral DNA and mediating the recruitment of chromatin silencers, such as the HUSH complex, SETDB1 and the histone deacetylases HDAC1 and HDAC4. Acts as an early regulator of adipogenesis by acting as a transcription cofactor of CEBPs (CEBPA, CEBPD and/or CEBPG), controlling the expression of PPARG and probably of other proadipogenic genes, such as SREBF1 (By similarity). May also regulate alternative splicing of target genes during adipogenesis (By similarity).

Subcellular Location:

Nucleus speckle.

Subunit Structure:

Interacts with FHL2. Interacts with CEBPA, CEBPD and CEBPG (By similarity). Interacts with MPHOSPH8 and TASOR components of the HUSH complex; leading to recruitment of the HUSH complex. Interacts with SETDB1. Interacts with HDAC1. Interacts with HDAC4.

Family&Domains:

The matrin-type zinc finger domain is required for localization to nuclear speckles.

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ZNF638 Antibody - #DF3280