Product: TSC22D1 Antibody
Catalog: DF3297
Description: Rabbit polyclonal antibody to TSC22D1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 16 KD; 110kD(Calculated).
Uniprot: Q15714
RRID: AB_2835665

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(82%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
TSC22D1 Antibody detects endogenous levels of total TSC22D1.
RRID:
AB_2835665
Cite Format: Affinity Biosciences Cat# DF3297, RRID:AB_2835665.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cerebral protein 2; DKFZp686O19206; Hucep 2; Hucep2; KIAA1994; MGC17597; OTTHUMP00000040978; Ptg 2; Regulatory protein TSC 22; Regulatory protein TSC-22; Regulatory protein TSC22; RP11 269C23.2; T22D1_HUMAN; TGFB stimulated clone 22 homolog; TGFB-stimulated clone 22 homolog; TGFB1I4; TGFbeta stimulated clone 22; Transcriptional regulator TSC 22; Transforming growth factor beta 1 induced transcript 4; Transforming growth factor beta 1 induced transcript 4 protein; Transforming growth factor beta stimulated protein TSC 22; Transforming growth factor beta-1-induced transcript 4 protein; TSC 22; TSC22; TSC22 domain family 1 isoform 2; TSC22 domain family member 1; TSC22 domain family protein 1; TSC22D1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q15714 T22D1_HUMAN:

Widely expressed in fetal and adult tissues.

Sequence:
MHQPPESTAAAAAAADISARKMAHPAMFPRRGSGSGSASALNAAGTGVGSNATSSEDFPPPSLLQPPPPAASSTSGPQPPPPQSLNLLSQAQLQAQPLAPGGTQMKKKSGFQITSVTPAQISASISSNNSIAEDTESYDDLDESHTEDLSSSEILDVSLSRATDLGEPERSSSEETLNNFQEAETPGAVSPNQPHLPQPHLPHLPQQNVVINGNAHPHHLHHHHQIHHGHHLQHGHHHPSHVAVASASITGGPPSSPVSRKLSTTGSSDSITPVAPTSAVSSSGSPASVMTNMRAPSTTGGIGINSVTGTSTVNNVNITAVGSFNPNVTSSMLGNVNISTSNIPSAAGVSVGPGVTSGVNVNILSGMGNGTISSSAAVSSVPNAAAGMTGGSVSSQQQQPTVNTSRFRVVKLDSSSEPFKKGRWTCTEFYEKENAVPATEGVLINKVVETVKQNPIEVTSERESTSGSSVSSSVSTLSHYTESVGSGEMGAPTVVVQQQQQQQQQQQQQPALQGVTLQQMDFGSTGPQSIPAVSIPQSISQSQISQVQLQSQELSYQQKQGLQPVPLQATMSAATGIQPSPVNVVGVTSALGQQPSISSLAQPQLPYSQAAPPVQTPLPGAPPPQQLQYGQQQPMVSTQMAPGHVKSVTQNPASEYVQQQPILQTAMSSGQPSSAGVGAGTTVIPVAQPQGIQLPVQPTAVPAQPAGASVQPVGQAPAAVSAVPTGSQIANIGQQANIPTAVQQPSTQVPPSVIQQGAPPSSQVVPPAQTGIIHQGVQTSAPSLPQQLVIASQSSLLTVPPQPQGVEPVAQGIVSQQLPAVSSLPSASSISVTSQVSSTGPSGMPSAPTNLVPPQNIAQTPATQNGNLVQSVSQPPLIATNTNLPLAQQIPLSSTQFSAQSLAQAIGSQIEDARRAAEPSLVGLPQTISGDSGGMSAVSDGSSSSLAASASLFPLKVLPLTTPLVDGEDESSSGASVVAIDNKIEQAMDLVKSHLMYAVREEVEVLKEQIKELIEKNSQLEQENNLLKTLASPEQLAQFQAQLQTGSPPATTQPQGTTQPPAQPASQGSGPTA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
100
Chicken
82
Xenopus
80
Zebrafish
55
Pig
0
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15714 As Substrate

Site PTM Type Enzyme
S256 Phosphorylation
S263 Phosphorylation
S288 Phosphorylation
T291 Phosphorylation
S414 Phosphorylation
S415 Phosphorylation
S936 Phosphorylation
K992 Ubiquitination
Y997 Phosphorylation
K1007 Ubiquitination
K1011 Ubiquitination
K1016 Ubiquitination
S1018 Phosphorylation
K1028 Ubiquitination
S1066 Phosphorylation

Research Backgrounds

Function:

Transcriptional repressor. Acts on the C-type natriuretic peptide (CNP) promoter.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in fetal and adult tissues.

Subunit Structure:

Homodimer or heterodimer. Can form a heterodimer with TSC22D4.

Family&Domains:

Belongs to the TSC-22/Dip/Bun family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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