NFIL3 Antibody - #DF3303
| Product: | NFIL3 Antibody |
| Catalog: | DF3303 |
| Description: | Rabbit polyclonal antibody to NFIL3 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Sheep, Rabbit, Dog |
| Mol.Wt.: | 51 KD; 51kD(Calculated). |
| Uniprot: | Q16649 |
| RRID: | AB_2835671 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3303, RRID:AB_2835671.
Fold/Unfold
E4 promoter binding protein 4; E4 promoter-binding protein 4; E4BP 4; E4BP4; E4BP4 protein; IL3BP 1; IL3BP1; Interleukin 3 binding protein 1; Interleukin 3 promoter transcriptional activator; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NFIL 3; NFIL 3A; NFIL3; NFIL3_HUMAN; NFIL3A; Nuclear factor interleukin 3 regulated; Nuclear factor interleukin-3-regulated protein; Transcriptional activator NF IL3A; Transcriptional activator NF-IL3A;
Immunogens
Expressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle.
- Q16649 NFIL3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQLRKMQTVKKEQASLDASSNVDKMMVLNSALTEVSEDSTTGEELLLSEGSVGKNKSSACRRKREFIPDEKKDAMYWEKRRKNNEAAKRSREKRRLNDLVLENKLIALGEENATLKAELLSLKLKFGLISSTAYAQEIQKLSNSTAVYFQDYQTSKSNVSSFVDEHEPSMVSSSCISVIKHSPQSSLSDVSEVSSVEHTQESSVQGSCRSPENKFQIIKQEPMELESYTREPRDDRGSYTASIYQNYMGNSFSGYSHSPPLLQVNRSSSNSPRTSETDDGVVGKSSDGEDEQQVPKGPIHSPVELKHVHATVVKVPEVNSSALPHKLRIKAKAMQIKVEAFDNEFEATQKLSSPIDMTSKRHFELEKHSAPSMVHSSLTPFSVQVTNIQDWSLKSEHWHQKELSGKTQNSFKTGVVEMKDSGYKVSDPENLYLKQGIANLSAEVVSLKRLIATQPISASDSG
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q16649 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S30 | Phosphorylation | Uniprot | |
| K72 | Sumoylation | Uniprot | |
| K82 | Acetylation | Uniprot | |
| K82 | Sumoylation | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K219 | Sumoylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| K306 | Sumoylation | Uniprot | |
| K337 | Sumoylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| K394 | Sumoylation | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| K406 | Sumoylation | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| K412 | Sumoylation | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| K419 | Sumoylation | Uniprot | |
| K419 | Ubiquitination | Uniprot | |
| K424 | Sumoylation | Uniprot | |
| K424 | Ubiquitination | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | Uniprot |
Research Backgrounds
Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death (By similarity). Represses the transcription of CYP2A5 (By similarity). Positively regulates the expression and activity of CES2 by antagonizing the repressive action of NR1D1 on CES2 (By similarity).
Nucleus.
Expressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle.
Homodimer. Binds DNA as a dimer. Interacts with DR1. Interacts with PER2 and CRY2 (By similarity). Interacts with NFIL3 and NR0B2 (By similarity).
Belongs to the bZIP family. NFIL3 subfamily.
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