Product: MARK2 Antibody
Catalog: DF3329
Description: Rabbit polyclonal antibody to MARK2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 85 KD; 88kD(Calculated).
Uniprot: Q7KZI7
RRID: AB_2835696

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
MARK2 Antibody detects endogenous levels of total MARK2.
Cite Format: Affinity Biosciences Cat# DF3329, RRID:AB_2835696.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


ELKL motif kinase 1; ELKL motif kinase; EMK-1; EMK1; MAP/microtubule affinity regulating kinase 2; MAP/microtubule affinity-regulating kinase 2; Mark2; MARK2_HUMAN; MGC99619; PAR 1; Par 1b; PAR1 homolog; Par1b; Ser/Thr protein kinase PAR 1B; Serine/threonine protein kinase EMK; Serine/threonine protein kinase MARK2; Serine/threonine-protein kinase MARK2;



High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.




Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7KZI7 As Substrate

Site PTM Type Enzyme
T6 Phosphorylation
T20 Phosphorylation
S26 Phosphorylation
K27 Ubiquitination
S29 Phosphorylation
S30 Phosphorylation
S40 Phosphorylation
T42 Phosphorylation
S43 Phosphorylation
T58 Phosphorylation
K61 Ubiquitination
K77 Ubiquitination
K82 Ubiquitination
K86 Ubiquitination
T87 Phosphorylation
S91 Phosphorylation Q14012 (CAMK1)
S92 Phosphorylation Q14012 (CAMK1)
S93 Phosphorylation Q14012 (CAMK1)
K177 Ubiquitination
S197 Phosphorylation
T201 Phosphorylation
T208 Phosphorylation Q7L7X3 (TAOK1) , Q15831 (STK11)
S212 Phosphorylation P49841 (GSK3B)
K224 Ubiquitination
S290 Phosphorylation
T294 Phosphorylation Q14012 (CAMK1)
K300 Ubiquitination
Y323 Phosphorylation
T329 Phosphorylation
Y337 Phosphorylation
T338 Phosphorylation
Y351 Phosphorylation
Y358 Phosphorylation
Y363 Phosphorylation
S365 Phosphorylation
T372 Phosphorylation
K376 Ubiquitination
S380 Phosphorylation
S386 Phosphorylation
S390 Phosphorylation
K394 Ubiquitination
S398 Phosphorylation
S400 Phosphorylation Q9BZL6 (PRKD2) , Q7KZI7 (MARK2) , Q15139 (PRKD1)
S409 Phosphorylation
S422 Phosphorylation
S449 Phosphorylation
S456 Phosphorylation
T466 Phosphorylation
T467 Phosphorylation
T469 Phosphorylation
S471 Phosphorylation
T472 Phosphorylation
S477 Phosphorylation
S479 Phosphorylation
S483 Phosphorylation
S486 Phosphorylation
S493 Phosphorylation
S498 Phosphorylation
S505 Phosphorylation
S514 Phosphorylation
S533 Phosphorylation
S535 Phosphorylation
S569 Phosphorylation
S571 Phosphorylation
S576 Phosphorylation
S578 Phosphorylation
R589 Methylation
S592 Phosphorylation
S593 Phosphorylation
S595 Phosphorylation
T596 Phosphorylation Q05513 (PRKCZ) , P41743 (PRKCI)
Y613 Phosphorylation
T616 Phosphorylation
S619 Phosphorylation
S621 Phosphorylation
S624 Phosphorylation
R628 Methylation
S631 Phosphorylation
S633 Phosphorylation
K637 Ubiquitination
K641 Ubiquitination
S648 Phosphorylation
T667 Phosphorylation
S675 Phosphorylation
S692 Phosphorylation
S698 Phosphorylation
K716 Ubiquitination
S722 Phosphorylation
S759 Phosphorylation

PTMs - Q7KZI7 As Enzyme

Substrate Site Source
P10636-8 (MAPT) S262 Uniprot
P10636-8 (MAPT) S356 Uniprot
P46939 (UTRN) S1258 Uniprot
P56524 (HDAC4) S246 Uniprot
P78352 (DLG4) S561 Uniprot
Q53ET0 (CRTC2) S171 Uniprot
Q53ET0 (CRTC2) S274 Uniprot
Q7KZI7 (MARK2) S400 Uniprot
Q7L804 (RAB11FIP2) S227 Uniprot
Q8TEW0 (PARD3) S144 Uniprot
Q8TEW0 (PARD3) S873 Uniprot
Q8WUI4 (HDAC7) S155 Uniprot
Q92974 (ARHGEF2) S143 Uniprot
Q92974 (ARHGEF2) S172 Uniprot
Q92974 (ARHGEF2) S186 Uniprot
Q92974-2 (ARHGEF2) S885 Uniprot
Q92974 (ARHGEF2) S886 Uniprot
Q92974-2 (ARHGEF2) S959 Uniprot
Q92974 (ARHGEF2) S960 Uniprot
Q9BXM7 (PINK1) T313 Uniprot
Q9H4P4 (RNF41) S254 Uniprot
Q9NQT8 (KIF13B) S1381 Uniprot
Q9NQT8 (KIF13B) S1410 Uniprot
Q9UQB8 (BAIAP2) S366 Uniprot
Q9UQL6 (HDAC5) S259 Uniprot

Research Backgrounds


Serine/threonine-protein kinase. Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU. Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.


Autophosphorylated. Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-208 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-212 by GSK3-beta (GSK3B) inhibits the kinase activity. Phosphorylation by CaMK1 promotes activity and is required to promote neurite outgrowth. Phosphorylation at Thr-596 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity and promotes binding to 14-3-3 protein YWHAZ, leading to relocation from cell membrane to cytoplasm.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Cytoplasm. Lateral cell membrane. Cytoplasm>Cytoskeleton. Cell projection>Dendrite. Cytoplasm.
Note: Phosphorylation at Thr-596 by PRKCZ/aPKC and subsequent interaction with 14-3-3 protein YWHAZ promotes relocation from the cell membrane to the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.

Subunit Structure:

Homodimer. Interacts with PAK5; leading to inhibit the protein kinase activity (By similarity). Interacts with MAPT/TAU. Interacts with MTCL1 isoform 1; the interaction is direct and increases MARK2 microtubule-binding ability. Interacts (when phosphorylated at Thr-596) with YWHAZ. Interacts with YWHAB, YWHAG and YWHAQ.

(Microbial infection) In case of infection, interacts with H.pylori CagA, leading to inhibit kinase activity and junctional and polarity defects.


The UBA domain does not seem to bind ubiquitin and ubiquitin-like and might play a role in regulating the enzyme conformation and localization. Activation of the kinase activity following phosphorylation at Thr-208 is accompanied by a conformational change that alters the orientation of the UBA domain with respect to the catalytic domain.

The KA1 domain mediates binding to phospholipids and targeting to membranes.

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.


1). Wei X et al. MARK2 enhances cisplatin resistance via PI3K/AKT/NF-κB signaling pathway in osteosarcoma cells. American Journal of Translational Research 2020 May 15;12(5):1807-1823. (PubMed: 32509178) [IF=2.2]

Application: IHC    Species: mouse    Sample: xenograft tumor

Figure 6. |MARK2 promotes cisplatin resistance of osteosarcoma cells in vivo. MNNG/HOS cells with stable knockdown or overexpression of MARK2 and their respective control cells were subcutaneously injected in nude mice. Ten days after injection with the indicated cells, mice were treated with cisplatin (3 mg/kg) or NS intraperitoneally every 3 days (n = 6 per group).F. IHC of MARK2, P-gp, and cleaved caspase 3 protein levels in xenograft tumor sections collected from mice treated with cisplatin. Magnification: 400 ×, scale bars: 100 μm. NS,normal saline; IHC, immunohistochemistry.

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