TBC1D3 Antibody - #DF3346
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3346, RRID:AB_2835713.
Fold/Unfold
PRC17; TBC1 domain family member 3; TBC1 domain family member 3F; TBC1D3; TBC1D3F; TBC3F_HUMAN; TRE17 alpha protein; TRE17;
Immunogens
Expressed in liver, skeletal muscle, kidney, pancreas, spleen, testis, ovary, small intestine and peripheral blood leukocytes. Overexpressed in prostate cancers.
Q6IPX1 TBC3C_HUMAN:Expressed in pancreas, thymus and testis.
- Q8IZP1 TBC3A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVVEVAGSWWAQEREDIIMKYEKGHRAGLPEDKGPKPFRSYNNNVDHLGIVHETELPPLTAREAKQIRREISRKSKWVDMLGDWEKYKSSRKLIDRAYKGMPMNIRGPMWSVLLNTEEMKLKNPGRYQIMKEKGKRSSEHIQRIDRDVSGTLRKHIFFRDRYGTKQRELLHILLAYEEYNPEVGYCRDLSHIAALFLLYLPEEDAFWALVQLLASERHSLQGFHSPNGGTVQGLQDQQEHVVATSQPKTMGHQDKKDLCGQCSPLGCLIRILIDGISLGLTLRLWDVYLVEGEQALMPITRIAFKVQQKRLTKTSRCGPWARFCNRFVDTWARDEDTVLKHLRASMKKLTRKKGDLPPPAKPEQGSSASRPVPASRGGKTLCKGDRQAPPGPPARFPRPIWSASPPRAPRSSTPCPGGAVREDTYPVGTQGVPSPALAQGGPQGSWRFLQWNSMPRLPTDLDVEGPWFRHYDFRQSCWVRAISQEDQLAPCWQAEHPAERVRSAFAAPSTDSDQGTPFRARDEQQCAPTSGPCLCGLHLESSQFPPGF
- A6NDS4 TBC3B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVVEVAGSWWAQEREDIIMKYEKGHRAGLPEDKGPKPFRSYNNNVDHLGIVHETELPPLTAREAKQIRREISRKSKWVDMLGDWEKYKSSRKLIDRAYKGMPMNIRGPMWSVLLNIEEMKLKNPGRYQIMKEKGKRSSEHIQRIDRDISGTLRKHMFFRDRYGTKQRELLHILLAYEEYNPEVGYCRDLSHIAALFLLYLPEEDAFWALVQLLASERHSLQGFHSPNGGTVQGLQDQQEHVVATSQSKTMGHQDKKDLCGQCSPLGCLIRILIDGISLGLTLRLWDVYLVEGEQALMPITRIAFKVQQKRLTKTSRCGPWARFCNRFVDTWARDEDTVLKHLRASMKKLTRKQGDLPPPAKPEQGSSASRPVPASRGRKTLCKGDRQAPPGPPARFPRPIWSASPPRAPRSSTPCPGGAVREDTYPVGTQGVPSPALAQGGPQGSWRFLQWNSMPRLPTDLDVEGPWFRHYDFRQSCWVRAISQEDQLAPCWQAEHPAERVRSAFAAPSTDSDQGTPFRARDEQQCAPTSGPCLCGLHLESSQFPPGF
- Q6IPX1 TBC3C_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVVEVAGSWWAQEREDIIMKYEKGHRAGLPEDKGPKPFRSYNNNVDHLGIVHETELPPLTAREAKQIRREISRKSKWVDMLGDWEKYKSSRKLIDRAYKGMPMNIRGPMWSVLLNTEEMKLKNPGRYQIMKEKGKRSSEHIQRIDRDVSGTLRKHIFFRDRYGTKQRELLHILLAYEEYNPEVGYCRDLSHIAALFLLYLPEEDAFWALVQLLASERHSLQGFHSPNGGTVQGLQDQQEHVVATSQPKTMGHQDKKDLCGQCSPLGCLIRILIDGISLGLTLRLWDVYLVEGEQALMPITRIAFKVQQKRLTKTSRCGPWARFCNRFVDTWARDEDTVLKHLRASMKKLTRKKGDLPPPAKPEQGSSASRPVPASRGGKTLCKGDRQAPPGPPARFPRPIWSASPPRAPRSSTPCPGGAVREDTYPVGTQGVPSPALAQGGPQGSWRFLQWNSMPRLPTDLDVEGPWFRHYDFRQSCWVRAISQEDQLAPCWQAEHPAERVRSAFAAPSTDSDQGTPFRARDEQQCAPTSGPCLCGLHLESSQFPPGF
PTMs - Q8IZP1/A6NDS4/Q6IPX1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y163 | Phosphorylation | Uniprot | |
| T165 | Phosphorylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| Y289 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y289 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y289 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot |
Research Backgrounds
Acts as a GTPase activating protein for RAB5. Does not act on RAB4 or RAB11.
Ubiquitinated by a CUL7-based E3 ligase, which leads to proteasomal degradation.
Palmitoylation is required for membrane localization and protects TBC1D3 from ubiquitination.
Cell membrane>Lipid-anchor.
Note: Associated with lipid rafts.
Expressed in liver, skeletal muscle, kidney, pancreas, spleen, testis, ovary, small intestine and peripheral blood leukocytes. Overexpressed in prostate cancers.
Acts as a GTPase activating protein for RAB5. Does not act on RAB4 or RAB11 (By similarity).
Ubiquitinated by a CUL7-based E3 ligase, which leads to proteasomal degradation.
Palmitoylation is required for membrane localization and protects TBC1D3 from ubiquitination.
Cell membrane>Lipid-anchor.
Note: Associated with lipid rafts.
Acts as a GTPase activating protein for RAB5. Does not act on RAB4 or RAB11 (By similarity).
Ubiquitinated by a CUL7-based E3 ligase, which leads to proteasomal degradation.
Palmitoylation is required for membrane localization and protects TBC1D3 from ubiquitination.
Cell membrane>Lipid-anchor.
Note: Associated with lipid rafts.
Expressed in pancreas, thymus and testis.
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