Product: PPP1R14C Antibody
Catalog: DF3361
Description: Rabbit polyclonal antibody to PPP1R14C
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Chicken, Xenopus
Mol.Wt.: 20 KD; 18kD(Calculated).
Uniprot: Q8TAE6
RRID: AB_2835719

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PPP1R14C Antibody detects endogenous levels of total PPP1R14C.
RRID:
AB_2835719
Cite Format: Affinity Biosciences Cat# DF3361, RRID:AB_2835719.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CPI17 like; KEPI; Kinase enhanced PP1 inhibitor; NY BR 81; PKC potentiated PP1 inhibitory protein; Protein phosphatase 1 regulatory subunit 14C; Serologically defined breast cancer antigen NY BR 81;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8TAE6 PP14C_HUMAN:

Detected in breast cancer.

Sequence:
MSVATGSSETAGGASGGGARVFFQSPRGGAGGSPGSSSGSGSSREDSAPVATAAAAGQVQQQQQRRHQQGKVTVKYDRKELRKRLVLEEWIVEQLGQLYGCEEEEMPEVEIDIDDLLDADSDEERASKLQEALVDCYKPTEEFIKELLSRIRGMRKLSPPQKKSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Xenopus
100
Zebrafish
100
Chicken
100
Sheep
0
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TAE6 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S25 Phosphorylation
R27 Methylation
S33 Phosphorylation
S43 Phosphorylation
T73 Phosphorylation P17612 (PRKACA) , Q13418 (ILK) , Q9P289 (STK26) , Q9Y6E0 (STK24)
K75 Acetylation
K75 Methylation
K156 Acetylation
S158 Phosphorylation
K162 Acetylation

Research Backgrounds

Function:

Inhibitor of the PP1 regulatory subunit PPP1CA.

PTMs:

Has over 600-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction (By similarity). The main inhibitory site appears to be Thr-73.

Subcellular Location:

Cytoplasm. Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in breast cancer.

Family&Domains:

Belongs to the PP1 inhibitor family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.