BLZF1 Antibody - #DF3432

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Product: BLZF1 Antibody
Catalog: DF3432
Description: Rabbit polyclonal antibody to BLZF1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 47 KD; 45kD(Calculated).
Uniprot: Q9H2G9
RRID: AB_2835732

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
BLZF1 Antibody detects endogenous levels of total BLZF1.
RRID:
AB_2835732
Cite Format: Affinity Biosciences Cat# DF3432, RRID:AB_2835732.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1700030G05Rik; basic leucine zipper nuclear factor 1 (JEM 1); Basic leucine zipper nuclear factor 1; BLZF1; Blzf1l; GO45_HUMAN; GOLGIN 45; Golgin-45; JEM 1; JEM 1s; JEM-1; JEM1; MGC22497; p45 basic leucine zipper nuclear factor; p45 basic leucine-zipper nuclear factor;

Immunogens

Immunogen:
Uniprot:

Q9H2G9(GO45_HUMAN) >>Visit HPA database.

Gene(ID):
BLZF1(8548)
Expression:
Q9H2G9 GO45_HUMAN:

Detected in adrenal gland (PubMed:9129147).

Sequence:
MTTKNLETKVTVTSSPIRGAGDGMETEEPPKSVEVTSGVQSRKHHSLQSPWKKAVPSESPGVLQLGKMLTEKAMEVKAVRILVPKAAITHDIPNKNTKVKSLGHHKGEFLGQSEGVIEPNKELSEVKNVLEKLKNSERRLLQDKEGLSNQLRVQTEVNRELKKLLVASVGDDLQYHFERLAREKNQLILENEALGRNTAQLSEQLERMSIQCDVWRSKFLASRVMADELTNSRAALQRQNRDAHGAIQDLLSEREQFRQEMIATQKLLEELLVSLQWGREQTYSPSVQPHSTAELALTNHKLAKAVNSHLLGNVGINNQKKIPSTVEFCSTPAEKMAETVLRILDPVTCKESSPDNPFFESSPTTLLATKKNIGRFHPYTRYENITFNCCNHCRGELIAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
88
Chicken
86
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H2G9 As Substrate

Site PTM Type Enzyme
T13 Phosphorylation
S15 Phosphorylation
T26 Phosphorylation
K31 Ubiquitination
S32 Phosphorylation
S37 Phosphorylation
K43 Ubiquitination
S49 Phosphorylation
K52 Ubiquitination
S59 Phosphorylation
K67 Ubiquitination
T70 Phosphorylation
K72 Ubiquitination
K77 Sumoylation
K77 Ubiquitination
K85 Ubiquitination
K95 Ubiquitination
K106 Ubiquitination
K121 Ubiquitination
K127 Sumoylation
K127 Ubiquitination
K144 Ubiquitination
K184 Ubiquitination
S284 Phosphorylation
K304 Ubiquitination
K320 Ubiquitination
K321 Ubiquitination
T331 Phosphorylation
T348 Phosphorylation
K350 Ubiquitination
S353 Phosphorylation
S362 Phosphorylation
T369 Phosphorylation
K370 Ubiquitination

Research Backgrounds

Function:

Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.

PTMs:

ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.

Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.

Subcellular Location:

Golgi apparatus membrane.

Nucleus.
Note: Detected in the nucleus upon heterologous expression. Not detected in the cytoplasm.

Cytoplasm.
Note: Not detected in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in adrenal gland.

Subunit Structure:

Interacts with GORASP2. Interacts with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. Identified in a complex with RAB2 and GORASP2.

Family&Domains:

The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.

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