AS250 Antibody - #DF3518
(3)
| Product: | AS250 Antibody |
| Catalog: | DF3518 |
| Description: | Rabbit polyclonal antibody to AS250 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 210 KD; 211kD(Calculated). |
| Uniprot: | Q2PPJ7 |
| RRID: | AB_2835738 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
AS250 Antibody detects endogenous levels of total AS250.
RRID:
AB_2835738
Cite Format: Affinity Biosciences Cat# DF3518, RRID:AB_2835738.
Cite Format: Affinity Biosciences Cat# DF3518, RRID:AB_2835738.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
250 kDa substrate of Akt; AS250; bA287B20.1; dJ1049G11; dJ1049G11.4; KIAA1272; p220; Ral GTPase-activating protein alpha subunit 2; Ral GTPase-activating protein subunit alpha-2; RALGAPA2; RapGAPalpha2; RGPA2_HUMAN; RP11-470C13.2;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q2PPJ7 RGPA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDANDLKQFFETNYSQIYFIFYENFIALENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVMSSRGPCTLETLINPSPSVADVKIYPEEITPLLPAISGEKIAEDQTCFFLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLDIPHLRPKPVYITTTRDNENIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAVQERAPELDGGGPTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRMVYEMVQRILLSTRGYVNFVNEVFHQAFLLPSCEIAVTRKVVQVYRKWILQDKPVFMEEPDRKDVAQEDAEKLGFSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEENTNVKAGVQALLQVFLTNSANIFLLEPCAEVPVLLKEQVDACKAVLIIFRRMIMELTMNKKTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLMVAWIRANLCVYISRELWDDFLGVLSSLTEWEELINEWANIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVLDPQKGTTVGRSFSLSWRSHPDVTEPMRFRSATTSGAPGVEKARNIVRQKATEVEECQQSENAPAAGSGHLTVGQQQQVLRSSSTSDIPEPLCSDSSQGQKAENTQNSSSSEPQPIQENKGHVKREHEGITILVRRSSSPAELDLKDDLQQTQGKCRERQKSESTNSDTTLGCTNEAELSMGPWQTCEEDPELNTPTDVVADADARHWLQLSPTDASNLTDSSECLTDDCSIIAGGSLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHARVFCYLYELWYKLAKIRDNLAISLDNQSSPSPPVLIPPLRMFASWLFKAATLPNEYKEGKLQAYRLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGFPGFSMLVGDFITAAARVLSTDILTAPRSEAVTVLGSLVCFPNTYQEIPLLQSVPEVNEAITGTEDVKHYLINILLKNATEEPNEYARCIAVCSLGVWICEELAQCTSHPQVKEAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKMAEILVATVAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSVLLHPVSTAVLEEQHSARAPLLDYIYRVLHCCVCGSSTYTQQSHYILTLADLSSTDYDPFLPLANVKSSEPVQYHSSAELGNLLTVEEEKKRRSLELIPLTARMVMAHLVNHLGHYPLSGGPAILHSLVSENHDNAHVEGSELSFEVFRSPNLQLFVFNDSTLISYLQTPTEGPVGGSPVGSLSDVRVIVRDISGKYSWDGKVLYGPLEGCLAPNGRNPSFLISSWHRDTFGPQKDSSQVEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSLTPCGMNYDQEKEIIEVILRQNAQEDEYIQSHNFDSAMKVTSQGQPSPVEPRGPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLVCATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSPSYSLSGTD
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q2PPJ7 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y316 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| Y388 | Phosphorylation | Uniprot | |
| K425 | Ubiquitination | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| T488 | Phosphorylation | Uniprot | |
| S492 | Phosphorylation | Uniprot | |
| K674 | Ubiquitination | Uniprot | |
| K675 | Ubiquitination | Uniprot | |
| K679 | Methylation | Uniprot | |
| K679 | Ubiquitination | Uniprot | |
| S694 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| S701 | Phosphorylation | Uniprot | |
| S713 | Phosphorylation | Uniprot | |
| T715 | Phosphorylation | Uniprot | |
| T716 | Phosphorylation | Uniprot | |
| S764 | Phosphorylation | Uniprot | |
| S765 | Phosphorylation | Uniprot | |
| S766 | Phosphorylation | Uniprot | |
| T767 | Phosphorylation | Uniprot | |
| S768 | Phosphorylation | Uniprot | |
| C775 | S-Nitrosylation | Uniprot | |
| T813 | Phosphorylation | Uniprot | |
| S819 | Phosphorylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| S821 | Phosphorylation | Uniprot | |
| S844 | Phosphorylation | Uniprot | |
| S846 | Phosphorylation | Uniprot | |
| T847 | Phosphorylation | Uniprot | |
| S849 | Phosphorylation | Uniprot | |
| S1493 | Phosphorylation | Uniprot | |
| K1508 | Ubiquitination | Uniprot | |
| S1593 | Phosphorylation | Uniprot | |
| Y1839 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Catalytic subunit of the heterodimeric RalGAP2 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB.
Subcellular Location:
Cytoplasm.
Subunit Structure:
Component of the heterodimeric RalGAP2 complex with RALGAPB. Heterodimerization is required for activity (By similarity).
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