CDH11 Antibody | Affinity Biosciences

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Cites

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Western blot analysis of extracts from Mouse brain, using CDH11 Antibody.

Figure 6.

Fig.

Product:	CDH11 Antibody
Catalog:	DF3523
Description:	Rabbit polyclonal antibody to CDH11
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	80,100kD; 88kD(Calculated).
Uniprot:	P55287
RRID:	AB_2835743

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)

Clonality:

Polyclonal

Specificity:

CDH11 Antibody detects endogenous levels of total CDH11.

RRID:

AB_2835743
Cite Format: Affinity Biosciences Cat# DF3523, RRID:AB_2835743.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CAD11; CAD11_HUMAN; Cadherin 11; Cadherin 11 type 2; Cadherin 11 type 2 OB cadherin (osteoblast); Cadherin-11; Cdh11; CDHOB; OB; OB-cadherin; OBcadherin; OSF-4; OSF4; Osteoblast cadherin;

Immunogens

Immunogen:

Uniprot:

P55287(CAD11_HUMAN) >>Visit HPA database.

Gene(ID):

CDH11(1009)

Expression:

P55287 CAD11_HUMAN:

Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts.

Sequence:

P55287 CAD11_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKENYCLQAALVCLGMLCHSHAFAPERRGHLRPSFHGHHEKGKEGQVLQRSKRGWVWNQFFVIEEYTGPDPVLVGRLHSDIDSGDGNIKYILSGEGAGTIFVIDDKSGNIHATKTLDREERAQYTLMAQAVDRDTNRPLEPPSEFIVKVQDINDNPPEFLHETYHANVPERSNVGTSVIQVTASDADDPTYGNSAKLVYSILEGQPYFSVEAQTGIIRTALPNMDREAKEEYHVVIQAKDMGGHMGGLSGTTKVTITLTDVNDNPPKFPQSVYQMSVSEAAVPGEEVGRVKAKDPDIGENGLVTYNIVDGDGMESFEITTDYETQEGVIKLKKPVDFETKRAYSLKVEAANVHIDPKFISNGPFKDTVTVKISVEDADEPPMFLAPSYIHEVQENAAAGTVVGRVHAKDPDAANSPIRYSIDRHTDLDRFFTINPEDGFIKTTKPLDREETAWLNITVFAAEIHNRHQEAKVPVAIRVLDVNDNAPKFAAPYEGFICESDQTKPLSNQPIVTISADDKDDTANGPRFIFSLPPEIIHNPNFTVRDNRDNTAGVYARRGGFSRQKQDLYLLPIVISDGGIPPMSSTNTLTIKVCGCDVNGALLSCNAEAYILNAGLSTGALIAILACIVILLVIVVLFVTLRRQKKEPLIVFEEEDVRENIITYDDEGGGEEDTEAFDIATLQNPDGINGFIPRKDIKPEYQYMPRPGLRPAPNSVDVDDFINTRIQEADNDPTAPPYDSIQIYGYEGRGSVAGSLSSLESATTDSDLDYDYLQNWGPRFKKLADLYGSKDTFDDDS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Zebrafish

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55287 As Substrate

P55287

Site	PTM Type	Source
K43	Acetylation	Uniprot
S93	Phosphorylation	Uniprot
T99	Phosphorylation	Uniprot
T219	Phosphorylation	Uniprot
K365	Ubiquitination	Uniprot
Y700	Phosphorylation	Uniprot
Y702	Phosphorylation	Uniprot
S714	Phosphorylation	Uniprot
T723	Phosphorylation	Uniprot
S788	Phosphorylation	Uniprot
T791	Phosphorylation	Uniprot

Research Backgrounds

P55287_CAD11_HUMAN

Function:

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts.

Subunit Structure:

Interacts with PCDH8.

Family&Domains:

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

References

1). MiRNA-451a inhibits airway remodeling by targeting Cadherin 11 in an allergic asthma model of neonatal mice. International Immunopharmacology, 2020 (PubMed: 32234673) [IF=5.6]

Application: IF/ICC Species: Mouse Sample: lung tissues

Fig. 1. Opposite expression of miRNA-451a and CDH11 in the lung of asthma mouse pups. (A) New born mice were intraperitoneally injected with 10 μg ovalbumin (OVA) from postnatal day 5 (P5) to P10, and followed by 10-min daily challenge with 3% aerosolized OVA from P18 to P20. Twenty-four hours post the last OVA challenge (P21), the bronchoalveolar lavage fluid (BALF) was collected, and then the mice were sacrificed to collect their lung tissues. CD 4+ T cells were isolated from the control and asthmatic BALF samples. The expression levels of miRNA-451a in (B) CD 4+ T cells and (C) lung tissues were determined with real-time qPCR. The mRNA and protein levels of CDH11 were determined with (D) real-time qPCR, (E) western blot, and (F) immunofluorescent staining (bars, 50 μm). Data were expressed as mean ± S.D., and p < 0.05 was regarded statistically significant. *, **, *** indicated a p < 0.05, 0.01, 0.001.

Application: WB Species: Mouse Sample: lung tissues

2). BCL2L10 inhibits growth and metastasis of hepatocellular carcinoma both in vitro and in vivo. MOLECULAR CARCINOGENESIS, 2017 (PubMed: 27770580) [IF=4.6]

Application: WB Species: human Sample: HepG2

Figure 6. Effect of BCL2L10 on its downstream gene expression profiles of human cancer pathway in HepG2 cells. (A) By human cancer pathway PCR array, ectopic expression of BCL2L10 up- or down-regulated several genes related to tumor proliferation, apoptosis, metastasis and angiogenesis. (B) Western blot was performed to confirm the downstream gene expression regulated by BCL2L10 in HepG2 cells. GAPDH was used as an internal control. (C) Schematic diagram of the molecular events for BCL2L10 function as a tumor suppressor through regulating cell cycle, proliferation, apoptosis metastasis and angiogenesis effectors.

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CDH11 Antibody - #DF3523