RIN3 Antibody - #DF3364
(2)
| Product: | RIN3 Antibody |
| Catalog: | DF3364 |
| Description: | Rabbit polyclonal antibody to RIN3 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human |
| Mol.Wt.: | 107 KD; 108kD(Calculated). |
| Uniprot: | Q8TB24 |
| RRID: | AB_2835746 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Clonality:
Polyclonal
Specificity:
RIN3 Antibody detects endogenous levels of total RIN3.
RRID:
AB_2835746
Cite Format: Affinity Biosciences Cat# DF3364, RRID:AB_2835746.
Cite Format: Affinity Biosciences Cat# DF3364, RRID:AB_2835746.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
DKFZp762H1613; FLJ11700; FLJ22439; Ras and Rab interactor 3; Ras interaction/interference protein 3;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q8TB24 RIN3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIRHAGAPARGDPTGPVPVVGKGEEEEEEDGMRLCLPANPKNCLPHRRGISILEKLIKTCPVWLQLSLGQAEVARILHRVVAGMFLVRRDSSSKQLVLCVHFPSLNESSAEVLEYTIKEEKSILYLEGSALVFEDIFRLIAFYCVSRDLLPFTLRLPQAILEASSFTDLETIANLGLGFWDSSLNPPQERGKPAEPPRDRAPGFPLVSSLRPTAHDANCACEIELSVGNDRLWFVNPIFIEDCSSALPTDQPPLGNCPARPLPPTSDATSPTSRWAPRRPPPPPPVLPLQPCSPAQPPVLPALAPAPACPLPTSPPVPAPHVTPHAPGPPDHPNQPPMMTCERLPCPTAGLGPLREEAMKPGAASSPLQQVPAPPLPAKKNLPTAPPRRRVSERVSLEDQSPGMAAEGDQLSLPPQGTSDGPEDTPRESTEQGQDTEVKASDPHSMPELPRTAKQPPVPPPRKKRISRQLASTLPAPLENAELCTQAMALETPTPGPPREGQSPASQAGTQHPPAQATAHSQSSPEFKGSLASLSDSLGVSVMATDQDSYSTSSTEEELEQFSSPSVKKKPSMILGKARHRLSFASFSSMFHAFLSNNRKLYKKVVELAQDKGSYFGSLVQDYKVYSLEMMARQTSSTEMLQEIRTMMTQLKSYLLQSTELKALVDPALHSEEELEAIVESALYKCVLKPLKEAINSCLHQIHSKDGSLQQLKENQLVILATTTTDLGVTTSVPEVPMMEKILQKFTSMHKAYSPEKKISILLKTCKLIYDSMALGNPGKPYGADDFLPVLMYVLARSNLTEMLLNVEYMMELMDPALQLGEGSYYLTTTYGALEHIKSYDKITVTRQLSVEVQDSIHRWERRRTLNKARASRSSVQDFICVSYLEPEQQARTLASRADTQAQALCAQCAEKFAVERPQAHRLFVLVDGRCFQLADDALPHCIKGYLLRSEPKRDFHFVYRPLDGGGGGGGGSPPCLVVREPNFL
PTMs - Q8TB24 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R3 | Methylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| T494 | Phosphorylation | Uniprot | |
| S572 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| S636 | Phosphorylation | Uniprot | |
| Y782 | Phosphorylation | Uniprot | |
| Y793 | Phosphorylation | Uniprot | |
| S850 | Phosphorylation | Uniprot | |
| S872 | Phosphorylation | Uniprot | |
| T893 | Phosphorylation | Uniprot | |
| S896 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Ras effector protein that functions as a guanine nucleotide exchange (GEF) for RAB5B and RAB31, by exchanging bound GDP for free GTP. Required for normal RAB31 function.
Subcellular Location:
Cytoplasm. Cytoplasmic vesicle. Early endosome.
Note: Activation of tyrosine phosphorylation signaling induces translocation to cytoplasmic vesicles.
Tissue Specificity:
Widely expressed.
Subunit Structure:
Interacts with CD2AP, RAB5B, RAB31 and BIN1.
Family&Domains:
Belongs to the RIN (Ras interaction/interference) family.
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For Research Use Only.
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