BRSK1 Antibody - #DF3366
| Product: | BRSK1 Antibody |
| Catalog: | DF3366 |
| Description: | Rabbit polyclonal antibody to BRSK1 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Dog |
| Mol.Wt.: | 87 KD; 85kD(Calculated). |
| Uniprot: | Q8TDC3 |
| RRID: | AB_2835748 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3366, RRID:AB_2835748.
Fold/Unfold
BR serine/threonine kinase 1; BR serine/threonine protein kinase 1; BR serine/threonine-protein kinase 1; Brain selective kinase 1; Brain-specific serine/threonine-protein kinase 1; BRSK 1; Brsk1; BRSK1_HUMAN; FLJ43009; hSAD1; KIAA1811; Protein kinase SAD1A; SAD 1; SAD1; SAD1 homolog; SAD1 kinase; SAD1A; SADB; SadB kinase short isoform; Serine/threonine kinase SAD B; Serine/threonine-protein kinase SAD-B; Synapses of Amphids Defective homolog;
Immunogens
Widely expressed, with highest levels in brain and testis. Protein levels remain constant throughout the cell cycle.
- Q8TDC3 BRSK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSGAKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGGGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSGGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGAPPRSLQPPPGRPDPELSSSPRRGPPKDKKLLATNGTPLP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8TDC3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K38 | Sumoylation | Uniprot | |
| T189 | Phosphorylation | Q15831 (STK11) , Q8N5S9 (CAMKK1) , Q8TDC3 (BRSK1) | Uniprot |
| S193 | Phosphorylation | Q8TDC3 (BRSK1) | Uniprot |
| S275 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| S404 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| T417 | Phosphorylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| R431 | Methylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| R481 | Methylation | Uniprot | |
| R484 | Methylation | Uniprot | |
| R498 | Methylation | Uniprot | |
| S508 | Phosphorylation | Uniprot | |
| R510 | Methylation | Uniprot | |
| T523 | Phosphorylation | Uniprot | |
| R525 | Methylation | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| T583 | Phosphorylation | Uniprot | |
| S587 | Phosphorylation | Uniprot | |
| S676 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | Uniprot | |
| T772 | Phosphorylation | Uniprot |
PTMs - Q8TDC3 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| P10636 (MAPT) | T529 | Uniprot |
| P10636 (MAPT) | S579 | Uniprot |
| P23258 (TUBG1) | S131 | Uniprot |
| P23258 (TUBG1) | S385 | Uniprot |
| P30291 (WEE1) | S642 | Uniprot |
| P30305 (CDC25B) | S375 | Uniprot |
| P30307 (CDC25C) | S216 | Uniprot |
| Q13131 (PRKAA1) | T183 | Uniprot |
| Q8TDC3 (BRSK1) | T189 | Uniprot |
| Q8TDC3 (BRSK1) | S193 | Uniprot |
| Q8TDC3-2 (BRSK1) | T205 | Uniprot |
| Q8TDC3-2 (BRSK1) | S209 | Uniprot |
Research Backgrounds
Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.
Phosphorylated at Thr-189 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not phosphorylated at Thr-189 by CaMKK2. In contrast, it is phosphorylated and activated by CaMKK1. May be inactivated via dephosphorylation of Thr-189 by PP2C.
Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cell junction>Synapse. Cell junction>Synapse>Presynaptic active zone. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle.
Note: Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage (By similarity).
Widely expressed, with highest levels in brain and testis. Protein levels remain constant throughout the cell cycle.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
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