MADD Antibody - #DF3374

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Product: MADD Antibody
Catalog: DF3374
Description: Rabbit polyclonal antibody to MADD
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 183 KD; 183kD(Calculated).
Uniprot: Q8WXG6
RRID: AB_2835756

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(80%), Bovine(92%), Horse(92%), Sheep(91%), Rabbit(92%), Dog(83%)
Clonality:
Polyclonal
Specificity:
MADD Antibody detects endogenous levels of total MADD.
RRID:
AB_2835756
Cite Format: Affinity Biosciences Cat# DF3374, RRID:AB_2835756.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Differentially expressed in normal and neoplastic cells; FLJ35600; IG20; Insulinoma glucagonoma clone 20; KIAA0358; MADD; MADD_HUMAN; MAP kinase-activating death domain protein; Rab3 GDP/GTP exchange factor; RAB3GEP;

Immunogens

Immunogen:
Uniprot:

Q8WXG6(MADD_HUMAN) >>Visit HPA database.

Gene(ID):
MADD(8567)
Expression:
Q8WXG6 MADD_HUMAN:

Highly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines.

Sequence:
MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Rabbit
92
Sheep
91
Dog
83
Zebrafish
80
Xenopus
67
Chicken
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WXG6 As Substrate

Site PTM Type Enzyme
S156 Phosphorylation
T228 Phosphorylation
S230 Phosphorylation
S262 Phosphorylation
K425 Ubiquitination
K429 Ubiquitination
K541 Ubiquitination
S613 Phosphorylation
S631 Phosphorylation
S636 Phosphorylation
S669 Phosphorylation
S689 Phosphorylation
S692 Phosphorylation
S709 Phosphorylation
S741 Phosphorylation
S745 Phosphorylation
S762 Phosphorylation
Y772 Phosphorylation
S801 Phosphorylation
S813 Phosphorylation
S818 Phosphorylation
S820 Phosphorylation
S824 Phosphorylation
S826 Phosphorylation
S829 Phosphorylation
S830 Phosphorylation
S848 Phosphorylation
S858 Phosphorylation
S860 Phosphorylation
S862 Phosphorylation
T867 Phosphorylation
K871 Methylation
K876 Ubiquitination
S882 Phosphorylation
K914 Ubiquitination
S916 Phosphorylation
K919 Ubiquitination
S921 Phosphorylation
T923 Phosphorylation
S930 Phosphorylation
K964 Ubiquitination
S972 Phosphorylation
S981 Phosphorylation
S1059 Phosphorylation
T1061 Phosphorylation
S1063 Phosphorylation
T1066 Phosphorylation
K1070 Ubiquitination
S1110 Phosphorylation
K1131 Acetylation
K1144 Acetylation
S1157 Phosphorylation
T1168 Phosphorylation
S1170 Phosphorylation
T1174 Phosphorylation
S1178 Phosphorylation
S1183 Phosphorylation
T1199 Phosphorylation
S1213 Phosphorylation
S1233 Phosphorylation
S1234 Phosphorylation
T1237 Phosphorylation
S1239 Phosphorylation
S1241 Phosphorylation
T1245 Phosphorylation
S1258 Phosphorylation
K1260 Ubiquitination
S1262 Phosphorylation
S1270 Phosphorylation
S1279 Phosphorylation
S1354 Phosphorylation
S1380 Phosphorylation
Y1381 Phosphorylation
Y1488 Phosphorylation
S1503 Phosphorylation
K1537 Ubiquitination
K1608 Ubiquitination

Research Backgrounds

Function:

Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.

Subcellular Location:

Membrane. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines.

Subunit Structure:

Interacts with the death domain of TNFRSF1A through its own death domain. Interacts with PIDD1. Interacts with YWHAZ.

Family&Domains:

Belongs to the MADD family.

Restrictive clause

 

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