SMG7 Antibody | Affinity Biosciences

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IHC
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Product:	SMG7 Antibody
Catalog:	DF3377
Description:	Rabbit polyclonal antibody to SMG7
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Horse, Sheep, Dog
Mol.Wt.:	127 KD; 127kD(Calculated).
Uniprot:	Q92540
RRID:	AB_2835759

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(83%), Horse(92%), Sheep(83%), Dog(92%)

Clonality:

Polyclonal

Specificity:

SMG7 Antibody detects endogenous levels of total SMG7.

RRID:

AB_2835759
Cite Format: Affinity Biosciences Cat# DF3377, RRID:AB_2835759.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

breast cancer-associated antigen SGA-56M; C1orf16; EST1 like protein C; EST1 telomerase component homolog C; EST1-like protein C; EST1C; ever shorter telomeres 1C; FLJ23717; hSMG-7; nonsense mediated mRNA decay factor (C. elegans); Protein SMG7; SGA56M; SMG 7; SMG-7 homolog; Smg7; SMG7_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q92540(SMG7_HUMAN) >>Visit HPA database.

Gene(ID):

SMG7(9887)

Sequence:

Q92540 SMG7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Dog

Pig

Sheep

Bovine

Chicken

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92540 As Substrate

Q92540

Site	PTM Type	Source
S2	Acetylation	Uniprot
K16	Ubiquitination	Uniprot
K23	Ubiquitination	Uniprot
K42	Ubiquitination	Uniprot
Y50	Phosphorylation	Uniprot
K54	Ubiquitination	Uniprot
K55	Ubiquitination	Uniprot
K66	Ubiquitination	Uniprot
K77	Ubiquitination	Uniprot
K122	Ubiquitination	Uniprot
K216	Ubiquitination	Uniprot
K228	Ubiquitination	Uniprot
K232	Ubiquitination	Uniprot
K243	Ubiquitination	Uniprot
K264	Ubiquitination	Uniprot
K268	Ubiquitination	Uniprot
K275	Ubiquitination	Uniprot
K438	Ubiquitination	Uniprot
K447	Ubiquitination	Uniprot
K464	Ubiquitination	Uniprot
S520	Phosphorylation	Uniprot
K524	Ubiquitination	Uniprot
S528	Phosphorylation	Uniprot
S534	Phosphorylation	Uniprot
T539	Phosphorylation	Uniprot
K542	Ubiquitination	Uniprot
K581	Acetylation	Uniprot
K585	Acetylation	Uniprot
T624	Phosphorylation	Uniprot
K780	Ubiquitination	Uniprot
S781	Phosphorylation	Uniprot
S786	Phosphorylation	Uniprot
Y791	Phosphorylation	Uniprot
K798	Ubiquitination	Uniprot
K812	Ubiquitination	Uniprot
K817	Ubiquitination	Uniprot
Y820	Phosphorylation	Uniprot
K847	Ubiquitination	Uniprot
K865	Ubiquitination	Uniprot
S897	Phosphorylation	Uniprot
R910	Methylation	Uniprot
S928	Phosphorylation	Uniprot
S929	Phosphorylation	Uniprot
S930	Phosphorylation	Uniprot
S938	Phosphorylation	Uniprot
R943	Methylation	Uniprot
Y954	Phosphorylation	Uniprot
K956	Ubiquitination	Uniprot
S1075	Phosphorylation	Uniprot
T1076	Phosphorylation	Uniprot
S1078	Phosphorylation	Uniprot
K1123	Ubiquitination	Uniprot

Research Backgrounds

Q92540_SMG7_HUMAN

Function:

Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Predominantly cytoplasmic, and nuclear. Shuttles between nucleus and cytoplasm.

Subunit Structure:

Part of a complex that contains SMG5, SMG7, PPP2CA, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated UPF1.

Research Fields

· Genetic Information Processing > Translation > mRNA surveillance pathway.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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SMG7 Antibody - #DF3377