AKAP1 Antibody - #DF3378

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Product: AKAP1 Antibody
Catalog: DF3378
Description: Rabbit polyclonal antibody to AKAP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 90 KD; 97kD(Calculated).
Uniprot: Q92667
RRID: AB_2835760

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
AKAP1 Antibody detects endogenous levels of total AKAP1.
RRID:
AB_2835760
Cite Format: Affinity Biosciences Cat# DF3378, RRID:AB_2835760.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A kinase anchor protein; A-kinase anchor protein 1; A-kinase anchor protein 149 kDa; AKAP 149; AKAP; Akap1; AKAP1_HUMAN; D-AKAP-1; DAKAP1; Dual specificity A-kinase-anchoring protein 1; mitochondrial; PRKA1; Protein kinase A-anchoring protein 1; Protein kinase A1; S-AKAP84; SAKAP84; Spermatid A-kinase anchor protein 84;

Immunogens

Immunogen:
Uniprot:

Q92667(AKAP1_HUMAN) >>Visit HPA database.

Gene(ID):
AKAP1(8165)
Expression:
Q92667 AKAP1_HUMAN:

Isoform 1 is detected in thymus, prostate, testis, ovary, colon and small intestine (PubMed:8769136). Isoform 2 is highly expressed in testis and detected at much lower levels in kidney, pancreas, liver, lung and brain (PubMed:7499250).

Sequence:
MAIQFRSLFPLALPGMLALLGWWWFFSRKKGHVSSHDEQQVEAGAVQLRADPAIKEPLPVEDVCPKVVSTPPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLRPGTRRDDSTKLELALTGGEAKSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQPGYPVVPAEKRSSGERARETGGAEGTGDAVLGEKVLEEALLSREHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELAKDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQIISQVISEATEQVLATTVGKVAGRVCQASQLQGQKEESCVPVHQKTVLGPDTAEPATAEAAVAPPDAGLPLPGLPAEGSPPPKTYVSCLKSLLSSPTKDSKPNISAHHISLASCLALTTPSEELPDRAGILVEDATCVTCMSDSSQSVPLVASPGHCSDSFSTSGLEDSCTETSSSPRDKAITPPLPESTVPFSNGVLKGELSDLGAEDGWTMDAEADHSGGSDRNSMDSVDSCCSLKKTESFQNAQAGSNPKKVDLIIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQSVQICHIEGSQHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADGAWWRAQVVASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVMPLSDDDQFSPEADAAMSEMTGNTALLAQVTSYSPTGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSYYTSL

PTMs - Q92667 As Substrate

Site PTM Type Enzyme
K55 Ubiquitination
C64 S-Nitrosylation
S69 Phosphorylation
T70 Phosphorylation
S73 Phosphorylation
S105 Phosphorylation
S107 Phosphorylation
S108 Phosphorylation
S128 Phosphorylation
S142 Phosphorylation
S150 Phosphorylation
S151 Phosphorylation
S158 Phosphorylation
S159 Phosphorylation
S161 Phosphorylation
S169 Phosphorylation
K213 Ubiquitination
S231 Phosphorylation
S235 Phosphorylation
S238 Phosphorylation
K266 Ubiquitination
S274 Phosphorylation
S309 Phosphorylation
S330 Phosphorylation
S353 Phosphorylation
S357 Phosphorylation
T360 Phosphorylation
T366 Phosphorylation
S429 Phosphorylation
K440 Methylation
S441 Phosphorylation
S444 Phosphorylation
S445 Phosphorylation
T447 Phosphorylation
T533 Phosphorylation
S573 Phosphorylation
S577 Phosphorylation
S580 Phosphorylation
S583 Phosphorylation
S586 Phosphorylation
K589 Ubiquitination
T590 Phosphorylation
S592 Phosphorylation
S600 Phosphorylation
K603 Ubiquitination
K634 Ubiquitination
K666 Ubiquitination
K673 Ubiquitination
K800 Ubiquitination
K803 Ubiquitination
Y900 Phosphorylation
S902 Phosphorylation

Research Backgrounds

Function:

Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.

Subcellular Location:

Mitochondrion outer membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is detected in thymus, prostate, testis, ovary, colon and small intestine. Isoform 2 is highly expressed in testis and detected at much lower levels in kidney, pancreas, liver, lung and brain.

Subunit Structure:

Interacts with SLC8A3.

Family&Domains:

RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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