REQU Antibody - #DF3380
| Product: | REQU Antibody |
| Catalog: | DF3380 |
| Description: | Rabbit polyclonal antibody to REQU |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 44 KD; 44kD(Calculated). |
| Uniprot: | Q92785 |
| RRID: | AB_2835762 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3380, RRID:AB_2835762.
Fold/Unfold
Apoptosis response zinc finger protein; BAF45D; BRG1-associated factor 45D; D4; D4 zinc and double PHD fingers family 2; double PHD fingers family 2; DPF 2; DPF2; MGC10180; Protein requiem; REQ; REQU_HUMAN; Requiem; Requiem apoptosis response zinc finger; UBI D4; ubi-d4; UBID 4; UBID4; zinc and double PHD fingers family 2; Zinc finger protein ubi d4; Zinc finger protein ubi-d4;
Immunogens
- Q92785 REQU_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAVVENVVKLLGEQYYKDAMEQCHNYNARLCAERSVRLPFLDSQTGVAQSNCYIWMEKRHRGPGLASGQLYSYPARRWRKKRRAHPPEDPRLSFPSIKPDTDQTLKKEGLISQDGSSLEALLRTDPLEKRGAPDPRVDDDSLGEFPVTNSRARKRILEPDDFLDDLDDEDYEEDTPKRRGKGKSKGKGVGSARKKLDASILEDRDKPYACDICGKRYKNRPGLSYHYAHSHLAEEEGEDKEDSQPPTPVSQRSEEQKSKKGPDGLALPNNYCDFCLGDSKINKKTGQPEELVSCSDCGRSGHPSCLQFTPVMMAAVKTYRWQCIECKCCNICGTSENDDQLLFCDDCDRGYHMYCLTPSMSEPPEGSWSCHLCLDLLKEKASIYQNQNSS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q92785 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K10 | Sumoylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| R38 | Methylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| R62 | Methylation | Uniprot | |
| Y72 | Phosphorylation | Uniprot | |
| R77 | Methylation | Uniprot | |
| R78 | Methylation | Uniprot | |
| R92 | Methylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| K99 | Sumoylation | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K107 | Sumoylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| T125 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| T176 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K178 | Ubiquitination | Uniprot | |
| K184 | Acetylation | Uniprot | |
| K186 | Acetylation | Uniprot | |
| K188 | Acetylation | Uniprot | |
| K195 | Acetylation | Uniprot | |
| K195 | Methylation | Uniprot | |
| K196 | Methylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| T248 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S251 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K261 | Acetylation | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| T358 | Phosphorylation | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| Y385 | Phosphorylation | Uniprot | |
| S390 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot |
Research Backgrounds
Plays an active role in transcriptional regulation by binding modified histones H3 and H4. Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells. Might also have a role in the development and maturation of lymphoid cells (By similarity). Involved in the regulation of non-canonical NF-kappa-B pathway.
Nucleus. Cytoplasm.
Ubiquitous.
Interacts with the nucleosomes, in particular nucleosomes bearing histone H3 crotonylated at 'Lys-14' (H3K14cr) for which DPF2 has high affinity. Also interacts (via PHD-type zinc finger domains) with histone H3 butyrylated at 'Lys-14' (H3K14bu), histone H3 propionylated at 'Lys-14' (H3K14pr), and histone H3 acetylated at 'Lys-14' (H3K14ac). Interacts with histone H3 acetylated at 'Lys-9' (H3K9ac), histone H3 di-methylated at 'Lys-9' (H3K9me2), and histone H3 tri-methylated at 'Lys-9' (H3K9me3). Interacts with histone H4 acetylated at 'Lys-12' (H4K12ac). Interacts with histone H4 acetylated at 'Lys-16' (H4K16ac). Interacts with SWI/SNF complex components. Interacts with SMARCA2, SMARCA4, SMARCB1 and SMARCD1. Interacts with SMARCC1, SMARCC2 and ACTL6A. Interacts with RUNX1.
Belongs to the requiem/DPF family.
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