Product: GCN5L2 Antibody
Catalog: DF3383
Description: Rabbit polyclonal antibody to GCN5L2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 100 KD; 94kD(Calculated).
Uniprot: Q92830
RRID: AB_2835765

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
GCN5L2 Antibody detects endogenous levels of total GCN5L2.
Cite Format: Affinity Biosciences Cat# DF3383, RRID:AB_2835765.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


1110051E14Rik; AW212720; EC; GCN 5; GCN5 (general control of amino-acid synthesis, yeast, homolog)-like 2; Gcn5; GCN5 general control of amino-acid synthesis 5-like 2 (yeast); GCN5L2; general control of amino acid synthesis 5-like 2; General control of amino acid synthesis protein 5-like 2; General control of amino acid synthesis, yeast, homolog-like 2; HGCN5; Histone acetyltransferase GCN5; Histone acetyltransferase KAT2A; hsGCN5; K(lysine) acetyltransferase 2A; KAT2 A; KAT2A; KAT2A_HUMAN; Lysine acetyltransferase 2A; MGC102791; PCAF-b; STAF97;



Expressed in all tissues tested, with most abundant expression in ovary.




Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92830 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K228 Sumoylation
T272 Phosphorylation
K289 Ubiquitination
S307 Phosphorylation
S372 Phosphorylation
K446 Ubiquitination
K549 Acetylation
K555 Ubiquitination
K669 Ubiquitination
K688 Ubiquitination
K697 Ubiquitination
K721 Acetylation
K721 Ubiquitination
K728 Ubiquitination
Y734 Phosphorylation
T735 Phosphorylation
T736 Phosphorylation
K759 Ubiquitination
K776 Ubiquitination
Y787 Phosphorylation
K791 Ubiquitination
K830 Ubiquitination

Research Backgrounds


Protein lysine acyltransferase that can act as a acetyltransferase, glutaryltransferase or succinyltransferase, depending on the context. Acts as a histone lysine succinyltransferase: catalyzes succinylation of histone H3 on 'Lys-79' (H3K79succ), with a maximum frequency around the transcription start sites of genes. Succinylation of histones gives a specific tag for epigenetic transcription activation. Association with the 2-oxoglutarate dehydrogenase complex, which provides succinyl-CoA, is required for histone succinylation. In different complexes, functions either as an acetyltransferase (HAT) or as a succinyltransferase: in the SAGA and ATAC complexes, acts as a histone acetyltransferase. Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. Acetylation of histones gives a specific tag for epigenetic transcription activation. Recruited by the XPC complex at promoters, where it specifically mediates acetylation of histone variant H2A.Z.1/H2A.Z, thereby promoting expression of target genes. Involved in long-term memory consolidation and synaptic plasticity: acts by promoting expression of a hippocampal gene expression network linked to neuroactive receptor signaling (By similarity). Acts as a positive regulator of T-cell activation: upon TCR stimulation, recruited to the IL2 promoter following interaction with NFATC2 and catalyzes acetylation of histone H3 at 'Lys-9' (H3K9ac), leading to promote IL2 expression (By similarity). Required for growth and differentiation of craniofacial cartilage and bone by regulating acetylation of histone H3 at 'Lys-9' (H3K9ac) (By similarity). Regulates embryonic stem cell (ESC) pluripotency and differentiation (By similarity). Also acetylates non-histone proteins, such as CEBPB, PLK4 and TBX5. Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5. Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4. Also acts as a histone glutaryltransferase: catalyzes glutarylation of histone H4 on 'Lys-91' (H4K91glu), a mark that destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes.

(Microbial infection) In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.

Subcellular Location:

Nucleus. Chromosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Mainly localizes to the nucleus (PubMed:27796307). Also localizes to centrosomes in late G1 and around the G1/S transition, coinciding with the onset of centriole formation (PubMed:27796307).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues tested, with most abundant expression in ovary.

Subunit Structure:

Homooligomer; may form a tetramer of homodimers. Interacts with EP300, CREBBP and ADA2. Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TAF3, TADA3L, SUPT3H/SPT3, TAF2/TAFII150, TAF4/TAFII135, TAF5/TAFII100, KAT2A/GCN5L2, TAF10 and TRRAP. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, KAT2A, SUPT7L, TAF5L, TAF6L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. The STAGA core complex is associated with a subcomplex required for histone deubiquitination composed of ATXN7L3, ENY2 and USP22. Component of the ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. In the complex, it probably interacts directly with KAT14, MBIP and WDR5. Interacts with PML (By similarity). Interacts with CEBPB. Interacts with TACC1, TACC2 and TACC3. Interacts with RELA (By similarity). Interacts with NFATC2 (By similarity). Interacts with TBX5. Interacts with PLK4. Associates with the 2-oxoglutarate dehydrogenase complex. Interacts with XPC; leading to KAT2A recruitment to promoters and subsequent acetylation of histones. Interacts with ERCC3/XPB; leading to KAT2A recruitment to promoters and subsequent acetylation of histones.

(Microbial infection) Interacts with and acetylates HIV-1 Tat.


Loop3 is required for substrate specificity and adopts different structural conformations in succinyl-CoA-bound and acetyl-CoA-bound forms. Tyr-645 has an important role in the selective binding of succinyl-CoA over acetyl-CoA.

Belongs to the acetyltransferase family. GCN5 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)


1). SIRT1 suppresses pituitary tumor progression by downregulating PTTG1 expression. ONCOLOGY REPORTS, 2022 (PubMed: 35730625) [IF=4.2]

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