BRF1 Antibody - #DF3388

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Product: BRF1 Antibody
Catalog: DF3388
Description: Rabbit polyclonal antibody to BRF1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Dog, Chicken
Mol.Wt.: 74 KD; 74kD(Calculated).
Uniprot: Q92994
RRID: AB_2835770

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(83%), Bovine(92%), Horse(100%), Dog(82%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
BRF1 Antibody detects endogenous levels of total BRF1.
RRID:
AB_2835770
Cite Format: Affinity Biosciences Cat# DF3388, RRID:AB_2835770.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B related factor 1; B-related factor 1; BRF; BRF-1; brf1; BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae); General transcription factor IIIB, 90kD subunit; GTF3B; hBRF; hTFIIIB90; RNA polymerase III; subunit 2; TAF3B2; TAF3C; TAFIII90; TATA box binding protein (TBP) associated factor 3C; TATA box binding protein (TBP) associated factor, RNA polymerase III, GTF3B subunit 2; TATA box-binding protein-associated factor; TBP associated factor, RNA polymerase III, 90kD; TF3B_HUMAN; TF3B90; TFIIIB90; Transcription factor IIIB 90 kDa subunit; Transcription initiation factor IIIB;

Immunogens

Immunogen:
Uniprot:

Q92994(TF3B_HUMAN) >>Visit HPA database.

Gene(ID):
BRF1(2972)
Sequence:
MTGRVCRGCGGTDIELDAARGDAVCTACGSVLEDNIIVSEVQFVESSGGGSSAVGQFVSLDGAGKTPTLGGGFHVNLGKESRAQTLQNGRRHIHHLGNQLQLNQHCLDTAFNFFKMAVSRHLTRGRKMAHVIAACLYLVCRTEGTPHMLLDLSDLLQVNVYVLGKTFLLLARELCINAPAIDPCLYIPRFAHLLEFGEKNHEVSMTALRLLQRMKRDWMHTGRRPSGLCGAALLVAARMHDFRRTVKEVISVVKVCESTLRKRLTEFEDTPTSQLTIDEFMKIDLEEECDPPSYTAGQRKLRMKQLEQVLSKKLEEVEGEISSYQDAIEIELENSRPKAKGGLASLAKDGSTEDTASSLCGEEDTEDEELEAAASHLNKDLYRELLGGAPGSSEAAGSPEWGGRPPALGSLLDPLPTAASLGISDSIRECISSQSSDPKDASGDGELDLSGIDDLEIDRYILNESEARVKAELWMRENAEYLREQREKEARIAKEKELGIYKEHKPKKSCKRREPIQASTAREAIEKMLEQKKISSKINYSVLRGLSSAGGGSPHREDAQPEHSASARKLSRRRTPASRSGADPVTSVGKRLRPLVSTQPAKKVATGEALLPSSPTLGAEPARPQAVLVESGPVSYHADEEADEEEPDEEDGEPCVSALQMMGSNDYGCDGDEDDGY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
92
Chicken
89
Pig
83
Dog
82
Xenopus
73
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92994 As Substrate

Site PTM Type Enzyme
T66 Phosphorylation
K79 Ubiquitination
K199 Ubiquitination
S226 Phosphorylation
K247 Ubiquitination
K254 Ubiquitination
T270 Phosphorylation
K304 Ubiquitination
K312 Ubiquitination
K313 Ubiquitination
Y324 Phosphorylation
K340 Ubiquitination
S345 Phosphorylation
T355 Phosphorylation
S357 Phosphorylation
S358 Phosphorylation
T365 Phosphorylation
S398 Phosphorylation
S433 Phosphorylation
S436 Phosphorylation
S450 Phosphorylation P53350 (PLK1)
K494 Ubiquitination
K496 Ubiquitination
S509 Phosphorylation
K537 Ubiquitination
S548 Phosphorylation
S553 Phosphorylation
K590 Acetylation
S614 Phosphorylation
T616 Phosphorylation

Research Backgrounds

Function:

General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

TFIIIB comprises at least the TATA-binding protein (TBP) and the B-related factor 1 (BRF1/TFIIIB90). Interacts with BDP1. Interacts with MAF1.

Family&Domains:

Belongs to the TFIIB family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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