PMEPA1 Antibody - #DF3391
| Product: | PMEPA1 Antibody |
| Catalog: | DF3391 |
| Description: | Rabbit polyclonal antibody to PMEPA1 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| Mol.Wt.: | 37 KD; 32kD(Calculated). |
| Uniprot: | Q969W9 |
| RRID: | AB_2835773 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3391, RRID:AB_2835773.
Fold/Unfold
PMEPA_HUMAN; PMEPA1; Prostate transmembrane protein, androgen induced 1; Solid tumor associated 1 protein; Solid tumor-associated 1 protein; STAG1; TMEPAI; Transmembrane prostate androgen induced protein; Transmembrane prostate androgen-induced protein; Transmembrane, prostate androgen induced RNA;
Immunogens
- Q969W9 PMEPA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MHRLMGVNSTAAAAAGQPNVSCTCNCKRSLFQSMEITELEFVQIIIIVVVMMVMVVVITCLLSHYKLSARSFISRHSQGRRREDALSSEGCLWPSESTVSGNGIPEPQVYAPPRPTDRLAVPPFAQRERFHRFQPTYPYLQHEIDLPPTISLSDGEEPPPYQGPCTLQLRDPEQQLELNRESVRAPPNRTIFDSDLMDSARLGGPCPPSSNSGISATCYGSGGRMEGPPPTYSEVIGHYPGSSFQHQQSSGPPSLLEGTRLHHTHIAPLESAAIWSKEKDKQKGHPL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q969W9 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T59 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| Y137 | Phosphorylation | Uniprot | |
| T217 | Phosphorylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| Y232 | Phosphorylation | Uniprot | |
| Y239 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot |
Research Backgrounds
Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal. Also involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4.
Early endosome membrane>Single-pass membrane protein. Golgi apparatus membrane>Single-pass membrane protein.
Highest expression in prostate. Also expressed in ovary.
Interacts with NEDD4 (via PPxY motifs). Interacts with AR. Interacts with LDLRAD4. Interacts (via the SMAD interaction motif) with SMAD2 and SMAD3.
The PPxY motifs mediate interaction with NEDD4.
The SMAD interaction motif is required for interaction with SMAD2 and SMAD3 and the negative regulation of TGF-beta signaling.
Belongs to the PMEPA1 family.
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