ISL2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from 293 cells, using ISL2 antibody.

Product:	ISL2 Antibody
Catalog:	DF3392
Description:	Rabbit polyclonal antibody to ISL2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	40 KD; 40kD(Calculated).
Uniprot:	Q96A47
RRID:	AB_2835774

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Chicken(82%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

ISL2 Antibody detects endogenous levels of total ISL2.

RRID:

AB_2835774
Cite Format: Affinity Biosciences Cat# DF3392, RRID:AB_2835774.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ10160; Insulin gene enhancer protein ISL 2; Insulin gene enhancer protein ISL-2; Insulin gene enhancer protein ISL2; ISL 2; ISL 2 transcription factor; ISL 2 transcription factor LIM/homeodomain; ISL LIM homeobox 2; ISL2; ISL2 transcription factor; ISL2 transcription factor LIM/homeodomain; ISL2_HUMAN; Islet-2; Islet2; SL2 transcription factor LIM/homeodomain (islet-2);

Immunogens

Immunogen:

Uniprot:

Q96A47(ISL2_HUMAN) >>Visit HPA database.

Gene(ID):

ISL2(64843)

Sequence:

Q96A47 ISL2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVDIIFHYPFLGAMGDHSKKKPGTAMCVGCGSQIHDQFILRVSPDLEWHAACLKCAECSQYLDETCTCFVRDGKTYCKRDYVRLFGIKCAKCQVGFSSSDLVMRARDSVYHIECFRCSVCSRQLLPGDEFSLREHELLCRADHGLLLERAAAGSPRSPGPLPGARGLHLPDAGSGRQPALRPHVHKQTEKTTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKKSILMKQLQQQQHSDKTSLQGLTGTPLVAGSPIRHENAVQGSAVEVQTYQPPWKALSEFALQSDLDQPAFQQLVSFSESGSLGNSSGSDVTSLSSQLPDTPNSMVPSPVET

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Rabbit

Chicken

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96A47 As Substrate

Q96A47

Site	PTM Type	Source
S43	Phosphorylation	Uniprot
S154	Phosphorylation	Uniprot
S157	Phosphorylation	Uniprot
K201	Ubiquitination	Uniprot
S231	Phosphorylation	Uniprot
S279	Phosphorylation	Uniprot

Research Backgrounds

Q96A47_ISL2_HUMAN

Function:

Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.

Subcellular Location:

Nucleus.

Subunit Structure:

Interacts with LHX4.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ISL2 Antibody - #DF3392