MASTL Antibody - #DF3398

(3)  
Product: MASTL Antibody
Catalog: DF3398
Description: Rabbit polyclonal antibody to MASTL
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 97 KD; 97kD(Calculated).
Uniprot: Q96GX5
RRID: AB_2835780

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
MASTL Antibody detects endogenous levels of total MASTL.
RRID:
AB_2835780
Cite Format: Affinity Biosciences Cat# DF3398, RRID:AB_2835780.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2700091H24Rik; C88295; FLJ14813; GREATWALL; Greatwall protein kinase; GW; GWL; GWL_HUMAN; hGWL; MAST-L; Mastl; MGC117975; Microtubule associated serine/threonine kinase like; Microtubule-associated serine/threonine-protein kinase-like; RP11 85G18.2; Serine/threonine-protein kinase greatwall; THC2;

Immunogens

Immunogen:
Uniprot:

Q96GX5(GWL_HUMAN) >>Visit HPA database.

Gene(ID):
MASTL(84930)
Sequence:
MDPTAGSKKEPGGGAATEEGVNRIAVPKPPSIEEFSIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMAKPRQDYSRTPGQVLSLISSLGFNTPIAEKNQDPANILSACLSETSQLSQGLVCPMSVDQKDTTPYSSKLLKSCLETVASNPGMPVKCLTSNLLQSRKRLATSSASSQSHTFISSVESECHSSPKWEKDCQESDEALGPTMMSWNAVEKLCAKSANAIETKGFNKKDLELALSPIHNSSALPTTGRSCVNLAKKCFSGEVSWEAVELDVNNINMDTDTSQLGFHQSNQWAVDSGGISEEHLGKRSLKRNFELVDSSPCKKIIQNKKTCVEYKHNEMTNCYTNQNTGLTVEVQDLKLSVHKSQQNDCANKENIVNSFTDKQQTPEKLPIPMIAKNLMCELDEDCEKNSKRDYLSSSFLCSDDDRASKNISMNSDSSFPGISIMESPLESQPLDSDRSIKESSFEESNIEDPLIVTPDCQEKTSPKGVENPAVQESNQKMLGPPLEVLKTLASKRNAVAFRSFNSHINASNNSEPSRMNMTSLDAMDISCAYSGSYPMAITPTQKRRSCMPHQQTPNQIKSGTPYRTPKSVRRGVAPVDDGRILGTPDYLAPELLLGRAHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAVEILLTIDDTKRAGMKELKRHPLFSDVDWENLQHQTMPFIPQPDDETDTSYFEARNTAQHLTVSGFSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
91
Zebrafish
91
Rabbit
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96GX5 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K9 Ubiquitination
T17 Phosphorylation
S31 Phosphorylation
K39 Ubiquitination
K48 Ubiquitination
K54 Ubiquitination
K57 Ubiquitination
K62 Ubiquitination
K66 Ubiquitination
K72 Ubiquitination
T75 Phosphorylation
S90 Phosphorylation
K158 Ubiquitination
S178 Phosphorylation
K179 Ubiquitination
T194 Phosphorylation
K199 Ubiquitination
T207 Phosphorylation
S216 Phosphorylation
S217 Phosphorylation
T222 Phosphorylation
K258 Sumoylation
T261 Phosphorylation
S264 Phosphorylation
S265 Phosphorylation
K266 Ubiquitination
K269 Methylation
K269 Ubiquitination
S270 Phosphorylation
T274 Phosphorylation
S277 Phosphorylation
K284 Ubiquitination
S288 Phosphorylation
S293 Phosphorylation
S303 Phosphorylation
S311 Phosphorylation
S319 Phosphorylation
S320 Phosphorylation
K322 Ubiquitination
S330 Phosphorylation
K350 Ubiquitination
K358 Ubiquitination
K363 Ubiquitination
S370 Phosphorylation
S375 Phosphorylation
S376 Phosphorylation
T380 Phosphorylation
S384 Phosphorylation
K390 Ubiquitination
S442 Phosphorylation
S452 Phosphorylation
S453 Phosphorylation
K497 Ubiquitination
K506 Ubiquitination
S512 Phosphorylation
T514 Phosphorylation
K516 Ubiquitination
T519 Phosphorylation
S550 Phosphorylation
S551 Phosphorylation
S552 Phosphorylation
S556 Phosphorylation
S597 Phosphorylation
S598 Phosphorylation
T611 Phosphorylation
T618 Phosphorylation
K621 Ubiquitination
S631 Phosphorylation
K634 Ubiquitination
K644 Acetylation
K644 Ubiquitination
S657 Phosphorylation
S660 Phosphorylation
S665 Phosphorylation
S668 Phosphorylation
S671 Phosphorylation
T696 Phosphorylation
T710 Phosphorylation
K715 Ubiquitination
T718 Phosphorylation
Y720 Phosphorylation
T722 Phosphorylation
S725 Phosphorylation
T741 Phosphorylation
T860 Phosphorylation
T868 Phosphorylation
T873 Phosphorylation
S875 Phosphorylation
S878 Phosphorylation

Research Backgrounds

Function:

Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.

PTMs:

Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity (By similarity). Maximum phosphorylation occurs in prometaphase.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Nucleus. Cleavage furrow.
Note: During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

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