MED24 Antibody | Affinity Biosciences

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MED24 Antibody - Western blot analysis of extracts from various samples, using MED24 Antibody.

Product:	MED24 Antibody
Catalog:	AF0346
Description:	Rabbit polyclonal antibody to MED24
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	110kDa; 110kD(Calculated).
Uniprot:	O75448
RRID:	AB_2833516

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(91%), Chicken(100%), Xenopus(91%)

Clonality:

Polyclonal

Specificity:

MED24 Antibody detects endogenous levels of total MED24.

RRID:

AB_2833516
Cite Format: Affinity Biosciences Cat# AF0346, RRID:AB_2833516.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CRSP complex subunit 4; Activator recruited cofactor 100 kDa component; Activator-recruited cofactor 100 kDa component; ARC100; Cofactor required for Sp1 transcriptional activation subunit 4; CRSP complex subunit 4; CRSP100; CRSP4; DRIP100; hTRAP100; KIAA0130; MED 24; MED24; MED24_HUMAN; Mediator complex subunit 24; Mediator of RNA polymerase II transcription subunit 24; Mediator of RNA polymerase II transcription subunit 24 homolog; MGC8748; OTTHUMP00000164458; OTTHUMP00000164460; THRAP4; Thyroid hormone receptor associated protein 4; Thyroid hormone receptor associated protein complex 100 kDa component; Thyroid hormone receptor-associated protein 4; Thyroid hormone receptor-associated protein complex 100 kDa component; Trap100; Trapp100; Vitamin D3 receptor interacting protein complex component DRIP100; Vitamin D3 receptor-interacting protein complex 100 kDa component;

Immunogens

Immunogen:

A synthesized peptide derived from human MED24, corresponding to a region within C-terminal amino acids.

Uniprot:

O75448(MED24_HUMAN) >>Visit HPA database.

Gene(ID):

MED24(9862)

Expression:

O75448 MED24_HUMAN:

Ubiquitous. Abundant in skeletal muscle, heart and placenta.

Description:

Trap100 a transcriptional coactivator protein of the thyroid hormone receptor-associated protein (TRAP) family. Forms a complex with the thyroid hormone receptor (TR) and markedly enhance TR-mediated transcription in vitro. Interacts and co-precipitates with Trap220 but does not directly bind nuclear hormone receptors.

Sequence:

O75448 MED24_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKVVNLKQAILQAWKERWSDYQWAINMKKFFPKGATWDILNLADALLEQAMIGPSPNPLILSYLKYAISSQMVSYSSVLTAISKFDDFSRDLCVQALLDIMDMFCDRLSCHGKAEECIGLCRALLSALHWLLRCTAASAERLREGLEAGTPAAGEKQLAMCLQRLEKTLSSTKNRALLHIAKLEEASSWTAIEHSLLKLGEILANLSNPQLRSQAEQCGTLIRSIPTMLSVHAEQMHKTGFPTVHAVILLEGTMNLTGETQSLVEQLTMVKRMQHIPTPLFVLEIWKACFVGLIESPEGTEELKWTAFTFLKIPQVLVKLKKYSHGDKDFTEDVNCAFEFLLKLTPLLDKADQRCNCDCTNFLLQECGKQGLLSEASVNNLMAKRKADREHAPQQKSGENANIQPNIQLILRAEPTVTNILKTMDADHSKSPEGLLGVLGHMLSGKSLDLLLAAAAATGKLKSFARKFINLNEFTTYGSEESTKPASVRALLFDISFLMLCHVAQTYGSEVILSESRTGAEVPFFETWMQTCMPEEGKILNPDHPCFRPDSTKVESLVALLNNSSEMKLVQMKWHEACLSISAAILEILNAWENGVLAFESIQKITDNIKGKVCSLAVCAVAWLVAHVRMLGLDEREKSLQMIRQLAGPLFSENTLQFYNERVVIMNSILERMCADVLQQTATQIKFPSTGVDTMPYWNLLPPKRPIKEVLTDIFAKVLEKGWVDSRSIHIFDTLLHMGGVYWFCNNLIKELLKETRKEHTLRAVELLYSIFCLDMQQVTLVLLGHILPGLLTDSSKWHSLMDPPGTALAKLAVWCALSSYSSHKGQASTRQKKRHREDIEDYISLFPLDDVQPSKLMRLLSSNEDDANILSSPTDRSMSSSLSASQLHTVNMRDPLNRVLANLFLLISSILGSRTAGPHTQFVQWFMEECVDCLEQGGRGSVLQFMPFTTVSELVKVSAMSSPKVVLAITDLSLPLGRQVAAKAIAAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Chicken

100

Dog

Xenopus

Zebrafish

Rabbit

Pig

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

O75448_MED24_HUMAN

Function:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Subcellular Location:

Nucleus.

Tissue Specificity:

Ubiquitous. Abundant in skeletal muscle, heart and placenta.

Family&Domains:

Belongs to the Mediator complex subunit 24 family.

Research Fields

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

References

1). Enhancer–promoter contact formation requires RNAPII and antagonizes loop extrusion. Nature Genetics, 2023 (PubMed: 37012454) [IF=31.7]

2). Dependency of 3D chromatin folding on RNA polymerase II at different cell cycle stages. , 2023

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For Research Use Only.
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MED24 Antibody - #AF0346