MED24 Antibody - #AF0346

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Product: MED24 Antibody
Catalog: AF0346
Description: Rabbit polyclonal antibody to MED24
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 110kDa; 110kD(Calculated).
Uniprot: O75448
RRID: AB_2833516

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(91%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
MED24 Antibody detects endogenous levels of total MED24.
RRID:
AB_2833516
Cite Format: Affinity Biosciences Cat# AF0346, RRID:AB_2833516.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CRSP complex subunit 4; Activator recruited cofactor 100 kDa component; Activator-recruited cofactor 100 kDa component; ARC100; Cofactor required for Sp1 transcriptional activation subunit 4; CRSP complex subunit 4; CRSP100; CRSP4; DRIP100; hTRAP100; KIAA0130; MED 24; MED24; MED24_HUMAN; Mediator complex subunit 24; Mediator of RNA polymerase II transcription subunit 24; Mediator of RNA polymerase II transcription subunit 24 homolog; MGC8748; OTTHUMP00000164458; OTTHUMP00000164460; THRAP4; Thyroid hormone receptor associated protein 4; Thyroid hormone receptor associated protein complex 100 kDa component; Thyroid hormone receptor-associated protein 4; Thyroid hormone receptor-associated protein complex 100 kDa component; Trap100; Trapp100; Vitamin D3 receptor interacting protein complex component DRIP100; Vitamin D3 receptor-interacting protein complex 100 kDa component;

Immunogens

Immunogen:
Uniprot:

O75448(MED24_HUMAN) >>Visit HPA database.

Gene(ID):
MED24(9862)
Expression:
O75448 MED24_HUMAN:

Ubiquitous. Abundant in skeletal muscle, heart and placenta.

Description:
Trap100 a transcriptional coactivator protein of the thyroid hormone receptor-associated protein (TRAP) family. Forms a complex with the thyroid hormone receptor (TR) and markedly enhance TR-mediated transcription in vitro. Interacts and co-precipitates with Trap220 but does not directly bind nuclear hormone receptors.
Sequence:
MKVVNLKQAILQAWKERWSDYQWAINMKKFFPKGATWDILNLADALLEQAMIGPSPNPLILSYLKYAISSQMVSYSSVLTAISKFDDFSRDLCVQALLDIMDMFCDRLSCHGKAEECIGLCRALLSALHWLLRCTAASAERLREGLEAGTPAAGEKQLAMCLQRLEKTLSSTKNRALLHIAKLEEASSWTAIEHSLLKLGEILANLSNPQLRSQAEQCGTLIRSIPTMLSVHAEQMHKTGFPTVHAVILLEGTMNLTGETQSLVEQLTMVKRMQHIPTPLFVLEIWKACFVGLIESPEGTEELKWTAFTFLKIPQVLVKLKKYSHGDKDFTEDVNCAFEFLLKLTPLLDKADQRCNCDCTNFLLQECGKQGLLSEASVNNLMAKRKADREHAPQQKSGENANIQPNIQLILRAEPTVTNILKTMDADHSKSPEGLLGVLGHMLSGKSLDLLLAAAAATGKLKSFARKFINLNEFTTYGSEESTKPASVRALLFDISFLMLCHVAQTYGSEVILSESRTGAEVPFFETWMQTCMPEEGKILNPDHPCFRPDSTKVESLVALLNNSSEMKLVQMKWHEACLSISAAILEILNAWENGVLAFESIQKITDNIKGKVCSLAVCAVAWLVAHVRMLGLDEREKSLQMIRQLAGPLFSENTLQFYNERVVIMNSILERMCADVLQQTATQIKFPSTGVDTMPYWNLLPPKRPIKEVLTDIFAKVLEKGWVDSRSIHIFDTLLHMGGVYWFCNNLIKELLKETRKEHTLRAVELLYSIFCLDMQQVTLVLLGHILPGLLTDSSKWHSLMDPPGTALAKLAVWCALSSYSSHKGQASTRQKKRHREDIEDYISLFPLDDVQPSKLMRLLSSNEDDANILSSPTDRSMSSSLSASQLHTVNMRDPLNRVLANLFLLISSILGSRTAGPHTQFVQWFMEECVDCLEQGGRGSVLQFMPFTTVSELVKVSAMSSPKVVLAITDLSLPLGRQVAAKAIAAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Chicken
100
Dog
91
Xenopus
91
Zebrafish
91
Rabbit
91
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75448 As Substrate

Site PTM Type Enzyme
T36 Phosphorylation
Y66 Phosphorylation
Y75 Phosphorylation
K156 Ubiquitination
K167 Ubiquitination
T172 Phosphorylation
K350 Ubiquitination
T360 Phosphorylation
S374 Phosphorylation
S377 Phosphorylation
K384 Ubiquitination
S397 Phosphorylation
K422 Ubiquitination
T423 Phosphorylation
S429 Phosphorylation
S444 Phosphorylation
S447 Phosphorylation
K460 Ubiquitination
K484 Ubiquitination
K638 Ubiquitination
S652 Phosphorylation
T655 Phosphorylation
K717 Ubiquitination
K721 Ubiquitination
K856 Ubiquitination
S862 Phosphorylation
S863 Phosphorylation
S872 Phosphorylation
S873 Phosphorylation
T875 Phosphorylation
K965 Ubiquitination
T971 Phosphorylation
K984 Ubiquitination

Research Backgrounds

Function:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Abundant in skeletal muscle, heart and placenta.

Subunit Structure:

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with AR.

Family&Domains:

Belongs to the Mediator complex subunit 24 family.

Research Fields

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

References

1). Enhancer–promoter contact formation requires RNAPII and antagonizes loop extrusion. Nature Genetics, 2023 (PubMed: 37012454) [IF=31.7]
2). Dependency of 3D chromatin folding on RNA polymerase II at different cell cycle stages. , 2023

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