UTP14A Antibody | Affinity Biosciences

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UTP14A Antibody - Figure 1 |IHC staining and Western blot analysis of UTP14a in esophageal squamous cell carcinoma (ESCC) and CDM tissues.

Product:	UTP14A Antibody
Catalog:	DF3418
Description:	Rabbit polyclonal antibody to UTP14A
Application:	WB IF/ICC
Cited expt.:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Horse, Dog
Mol.Wt.:	85 KD; 88kD(Calculated).
Uniprot:	Q9BVJ6
RRID:	AB_2835800

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(88%), Horse(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

UTP14A Antibody detects endogenous levels of total UTP14A.

RRID:

AB_2835800
Cite Format: Affinity Biosciences Cat# DF3418, RRID:AB_2835800.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Antigen NY CO 16; KIAA0266; SDCCAG16; Serologically defined colon cancer antigen 16; U3 small nucleolar RNA associated protein 14 homolog A; UTP14;

Immunogens

Immunogen:

A synthesized peptide derived from human UTP14A, corresponding to a region within N-terminal amino acids.

Uniprot:

Q9BVJ6(UT14A_HUMAN) >>Visit HPA database.

Gene(ID):

UTP14A(10813)

Expression:

Q9BVJ6 UT14A_HUMAN:

Ubiquitously expressed.

Sequence:

Q9BVJ6 UT14A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTANRLAESLLALSQQEELADLPKDYLLSESEDEGDNDGERKHQKLLEAISSLDGKNRRKLAERSEASLKVSEFNVSSEGSGEKLVLADLLEPVKTSSSLATVKKQLSRVKSKKTVELPLNKEEIERIHREVAFNKTAQVLSKWDPVVLKNRQAEQLVFPLEKEEPAIAPIEHVLSGWKARTPLEQEIFNLLHKNKQPVTDPLLTPVEKASLRAMSLEEAKMRRAELQRARALQSYYEAKARREKKIKSKKYHKVVKKGKAKKALKEFEQLRKVNPAAALEELEKIEKARMMERMSLKHQNSGKWAKSKAIMAKYDLEARQAMQEQLSKNKELTQKLQVASESEEEEGGTEDVEELLVPDVVNEVQMNADGPNPWMLRSCTSDTKEAATQEDPEQLPELEAHGVSESEGEERPVAEEEILLREFEERRSLRKRSELSQDAEPAGSQETKDSGSQEVLSELRVLSQKLKENHQSRKQKASSEGTIPQVQREEPAPEEEEPLLLQRPERVQTLEELEELGKEECFQNKELPRPVLEGQQSERTPNNRPDAPKEKKKKEQMIDLQNLLTTQSPSVKSLAVPTIEELEDEEERNHRQMIKEAFAGDDVIRDFLKEKREAVEASKPKDVDLTLPGWGEWGGVGLKPSAKKRRRFLIKAPEGPPRKDKNLPNVIINEKRNIHAAAHQVRVLPYPFTHHWQFERTIQTPIGSTWNTQRAFQKLTTPKVVTKPGHIINPIKAEDVGYRSSSRSDLSVIQRNPKRITTRHKKQLKKCSVD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Dog

Bovine

Rabbit

Chicken

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q9BVJ6_UT14A_HUMAN

Function:

May be required for ribosome biogenesis.

PTMs:

Citrullinated by PADI4.

Subcellular Location:

Nucleus>Nucleolus.

Tissue Specificity:

Ubiquitously expressed.

Family&Domains:

Belongs to the UTP14 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

References

1). Overexpression of U three protein 14a (UTP14a) is associated with poor prognosis of esophageal squamous cell carcinoma. Thoracic Cancer, 2019 (PubMed: 31496055) [IF=2.3]

Application: IHC Species: human Sample: esophageal squamous cell carcinoma (ESCC) and CDM tissues

Figure 1 |IHC staining and Western blot analysis of UTP14a in esophageal squamous cell carcinoma (ESCC) and CDM tissues. (a–d) Negative to strong UTP14a expression in ESCC tissues.

Application: WB Species: human Sample: ESCC and CDM tissue

Figure 1 |IHC staining and Western blot analysis of UTP14a in esophageal squamous cell carcinoma (ESCC) and CDM tissues. (a–d) Negative to strong UTP14a expression in ESCC tissues.(e–f) Negative-to-moderate UTP14a expression in CDM tissues. (h) Western blot analysis of six pairs of ESCC and CDM tissue.

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UTP14A Antibody - #DF3418