AN30A Antibody - #DF3421
(6)
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Protocols
Product Info
Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Clonality:
Polyclonal
Specificity:
AN30A Antibody detects endogenous levels of total AN30A.
RRID:
AB_2835803
Cite Format: Affinity Biosciences Cat# DF3421, RRID:AB_2835803.
Cite Format: Affinity Biosciences Cat# DF3421, RRID:AB_2835803.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
AN30A_HUMAN; ANKRD30A; ankyrin repeat domain 30A; Ankyrin repeat domain containing protein 30A; Ankyrin repeat domain-containing protein 30A; breast cancer antigen NY-BR-1; NY BR 1; RP11 20F24.1; Serologically defined breast cancer antigen NY BR 1; Serologically defined breast cancer antigen NY-BR-1;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9BXX3 AN30A_HUMAN:
Mainly expressed in breast and testis. A very faint signal is detected in placenta. Also expressed in many breast cancer cells.
Sequence:
- Q9BXX3 AN30A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEEISAAAVKVVPGPERPSPFSQLVYTSNDSYIVHSGDLRKIHKAASRGQVRKLEKMTKRKKTINLNIQDAQKRTALHWACVNGHEEVVTFLVDRKCQLDVLDGEHRTPLMKALQCHQEACANILIDSGADINLVDVYGNTALHYAVYSEILSVVAKLLSHGAVIEVHNKASLTPLLLSITKRSEQIVEFLLIKNANANAVNKYKCTALMLAVCHGSSEIVGMLLQQNVDVFAADICGVTAEHYAVTCGFHHIHEQIMEYIRKLSKNHQNTNPEGTSAGTPDEAAPLAERTPDTAESLVEKTPDEAAPLVERTPDTAESLVEKTPDEAASLVEGTSDKIQCLEKATSGKFEQSAEETPREITSPAKETSEKFTWPAKGRPRKIAWEKKEDTPREIMSPAKETSEKFTWAAKGRPRKIAWEKKETPVKTGCVARVTSNKTKVLEKGRSKMIACPTKESSTKASANDQRFPSESKQEEDEEYSCDSRSLFESSAKIQVCIPESIYQKVMEINREVEEPPKKPSAFKPAIEMQNSVPNKAFELKNEQTLRADPMFPPESKQKDYEENSWDSESLCETVSQKDVCLPKATHQKEIDKINGKLEESPNKDGLLKATCGMKVSIPTKALELKDMQTFKAEPPGKPSAFEPATEMQKSVPNKALELKNEQTLRADEILPSESKQKDYEENSWDTESLCETVSQKDVCLPKAAHQKEIDKINGKLEGSPVKDGLLKANCGMKVSIPTKALELMDMQTFKAEPPEKPSAFEPAIEMQKSVPNKALELKNEQTLRADEILPSESKQKDYEESSWDSESLCETVSQKDVCLPKATHQKEIDKINGKLEESPDNDGFLKAPCRMKVSIPTKALELMDMQTFKAEPPEKPSAFEPAIEMQKSVPNKALELKNEQTLRADQMFPSESKQKKVEENSWDSESLRETVSQKDVCVPKATHQKEMDKISGKLEDSTSLSKILDTVHSCERARELQKDHCEQRTGKMEQMKKKFCVLKKKLSEAKEIKSQLENQKVKWEQELCSVRLTLNQEEEKRRNADILNEKIREELGRIEEQHRKELEVKQQLEQALRIQDIELKSVESNLNQVSHTHENENYLLHENCMLKKEIAMLKLEIATLKHQYQEKENKYFEDIKILKEKNAELQMTLKLKEESLTKRASQYSGQLKVLIAENTMLTSKLKEKQDKEILEAEIESHHPRLASAVQDHDQIVTSRKSQEPAFHIAGDACLQRKMNVDVSSTIYNNEVLHQPLSEAQRKSKSLKINLNYAGDALRENTLVSEHAQRDQRETQCQMKEAEHMYQNEQDNVNKHTEQQESLDQKLFQLQSKNMWLQQQLVHAHKKADNKSKITIDIHFLERKMQHHLLKEKNEEIFNYNNHLKNRIYQYEKEKAETENS
PTMs - Q9BXX3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T63 | Phosphorylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| T271 | Phosphorylation | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| K422 | Acetylation | Uniprot | |
| K427 | Acetylation | Uniprot | |
| T428 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| T439 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| K734 | Ubiquitination | Uniprot | |
| S736 | Phosphorylation | Uniprot | |
| T749 | Phosphorylation | Uniprot | |
| S814 | Phosphorylation | Uniprot | |
| S839 | Phosphorylation | Uniprot | |
| S855 | Phosphorylation | Uniprot | |
| T868 | Phosphorylation | Uniprot | |
| K1019 | Ubiquitination | Uniprot | |
| Y1132 | Phosphorylation | Uniprot | |
| S1165 | Phosphorylation | Uniprot |
Research Backgrounds
Tissue Specificity:
Mainly expressed in breast and testis. A very faint signal is detected in placenta. Also expressed in many breast cancer cells.
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