BRMS1 Antibody - #DF3442
| Product: | BRMS1 Antibody |
| Catalog: | DF3442 |
| Description: | Rabbit polyclonal antibody to BRMS1 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Horse, Sheep, Dog |
| Mol.Wt.: | 28 KD; 28kD(Calculated). |
| Uniprot: | Q9HCU9 |
| RRID: | AB_2835808 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3442, RRID:AB_2835808.
Fold/Unfold
AV003220; AW554636; Breast cancer metastasis suppressor 1; Breast cancer metastasis-suppressor 1; BRMS1; BRMS1_HUMAN; DKFZp564A063; MGC95128;
Immunogens
Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies.
- Q9HCU9 BRMS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPVQPPSKDTEEMEAEGDSAAEMNGEEEESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQRSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKLLLYDTLQGELQERIQRLEEDRQSLDLSSEWWDDKLHARGSSRSWDSLPPSKRKKAPLVSGPYIVYMLQEIDILEDWTAIKKARAAVSPQKRKSDGP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9HCU9 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S30 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot |
Research Backgrounds
Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
Ubiquitinated by a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing SPOP, leading to proteasomal degradation.
Nucleus. Cytoplasm.
Note: Predominantly nuclear.
Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies.
Homohexamer (Potential). Interacts with SNX6, HDAC1 and RELA. Interacts with ARID4A. Identified in mSin3A corepressor complexes together with SIN3A, SIN3B, RBBP4, RBBP7, SAP30, SUDS3, ARID4A, HDAC1 and HDAC2. Interacts with SPOP; this recruits the protein to a ubiquitin ligase complex containing SPOP and CUL3.
Contains an N-terminal anti-parallel coiled coil formed by two BRMS1 chains; this region can form homohexamers.
Belongs to the BRMS1 family.
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