CREBZF Antibody - #DF3447

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Product: CREBZF Antibody
Catalog: DF3447
Description: Rabbit polyclonal antibody to CREBZF
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 26 KD; 37kD(Calculated).
Uniprot: Q9NS37
RRID: AB_2835813

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(86%), Bovine(86%), Sheep(86%), Rabbit(86%), Dog(86%)
Clonality:
Polyclonal
Specificity:
CREBZF Antibody detects endogenous levels of total CREBZF.
RRID:
AB_2835813
Cite Format: Affinity Biosciences Cat# DF3447, RRID:AB_2835813.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1110034C16Rik; 6330417B10Rik; AI225749; AI606244; CREB/ATF bZIP transcription factor; Crebzf; FLJ94018; HCF-binding transcription factor Zhangfei; Host cell factor-binding transcription factor Zhangfei; LAZip; LAZipII; SHP-interacting leucine zipper protein; SMILE; ZF; ZHANG_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q9NS37(ZHANG_HUMAN) >>Visit HPA database.

Gene(ID):
CREBZF(58487)
Expression:
Q9NS37 ZHANG_HUMAN:

In adults, expressed most abundantly in heart, liver and skeletal muscle, moderately abundant in kidney and pancreas, and barely detectable in lung. In fetal tissues, expressed most abundantly in kidney and very low amounts in heart, lung and liver.

Sequence:
MRHSLTKLLAASGSNSPTRSESPEPAATCSLPSDLTRAAAGEEETAAAGSPGRKQQFGDEGELEAGRGSRGGVAVRAPSPEEMEEEAIASLPGEETEDMDFLSGLELADLLDPRQPDWHLDPGLSSPGPLSSSGGGSDSGGLWRGDDDDEAAAAEMQRFSDLLQRLLNGIGGCSSSSDSGSAEKRRRKSPGGGGGGGSGNDNNQAATKSPRKAAAAAARLNRLKKKEYVMGLESRVRGLAAENQELRAENRELGKRVQALQEESRYLRAVLANETGLARLLSRLSGVGLRLTTSLFRDSPAGDHDYALPVGKQKQDLLEEDDSAGGVCLHVDKDKVSVEFCSACARKASSSLKM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
86
Bovine
86
Sheep
86
Dog
86
Rabbit
86
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NS37 As Substrate

Site PTM Type Enzyme
S14 Phosphorylation
S16 Phosphorylation
S20 Phosphorylation
S22 Phosphorylation
T28 Phosphorylation
S30 Phosphorylation
T45 Phosphorylation
S50 Phosphorylation
S174 Phosphorylation
S179 Phosphorylation
K184 Ubiquitination
S189 Phosphorylation
S198 Phosphorylation
T207 Phosphorylation
K208 Ubiquitination
S209 Phosphorylation
T275 Phosphorylation
S282 Phosphorylation
S285 Phosphorylation
S294 Phosphorylation
S299 Phosphorylation
K312 Ubiquitination

Research Backgrounds

Function:

Strongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes in CREBZF-expressing cells.

Subcellular Location:

Nucleus.
Note: Colocalizes in promyelocytic leukemia protein nuclear bodies (PML-NB) with CREB3 and HCFC1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

In adults, expressed most abundantly in heart, liver and skeletal muscle, moderately abundant in kidney and pancreas, and barely detectable in lung. In fetal tissues, expressed most abundantly in kidney and very low amounts in heart, lung and liver.

Subunit Structure:

Interacts with HCFC1; the interaction inhibits CREB3 transcriptional activity. Interacts with CREB3; the interaction occurs only in combination with HCFC1.

Family&Domains:

Belongs to the bZIP family. ATF subfamily.

Restrictive clause

 

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