AATF Antibody - #DF3453
| Product: | AATF Antibody |
| Catalog: | DF3453 |
| Description: | Rabbit polyclonal antibody to AATF |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Rabbit, Dog |
| Mol.Wt.: | 70kD; 63kD(Calculated). |
| Uniprot: | Q9NY61 |
| RRID: | AB_2835819 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3453, RRID:AB_2835819.
Fold/Unfold
AATF; AATF_HUMAN; Apoptosis antagonizing transcription factor; Apoptosis-antagonizing transcription factor; BFR2; CHE 1; CHE1; DED; Protein AATF; Rb binding protein Che 1; Rb-binding protein Che-1;
Immunogens
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
- Q9NY61 AATF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGPQPLALQLEQLLNPRPSEADPEADPEEATAARVIDRFDEGEDGEGDFLVVGSIRKLASASLLDTDKRYCGKTTSRKAWNEDHWEQTLPGSSDEEISDEEGSGDEDSEGLGLEEYDEDDLGAAEEQECGDHRESKKSRSHSAKTPGFSVQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDDAEDSQGESEEDRAGDRNSEDDGVVMTFSSVKVSEEVEKGRAVKNQIALWDQLLEGRIKLQKALLTTNQLPQPDVFPLFKDKGGPEFSSALKNSHKALKALLRSLVGLQEELLFQYPDTRYLVDGTKPNAGSEEISSEDDELVEEKKQQRRRVPAKRKLEMEDYPSFMAKRFADFTVYRNRTLQKWHDKTKLASGKLGKGFGAFERSILTQIDHILMDKERLLRRTQTKRSVYRVLGKPEPAAQPVPESLPGEPEILPQAPANAHLKDLDEEIFDDDDFYHQLLRELIERKTSSLDPNDQVAMGRQWLAIQKLRSKIHKKVDRKASKGRKLRFHVLSKLLSFMAPIDHTTMNDDARTELYRSLFGQLHPPDEGHGD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NY61 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| K58 | Sumoylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| T67 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| Y117 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| S143 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| K145 | Ubiquitination | Uniprot | |
| T146 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S203 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| K223 | Sumoylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K276 | Methylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| Y305 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| K342 | Sumoylation | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| T366 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
| K369 | Acetylation | Uniprot | |
| K380 | Acetylation | Uniprot | |
| K383 | Acetylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| T410 | Phosphorylation | Uniprot | |
| T412 | Phosphorylation | Uniprot | |
| K422 | Sumoylation | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| K451 | Ubiquitination | Uniprot | |
| K475 | Sumoylation | Uniprot | |
| K475 | Ubiquitination | Uniprot | |
| T476 | Phosphorylation | Uniprot | |
| S477 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| K496 | Ubiquitination | Uniprot | |
| S510 | Phosphorylation | O96017 (CHEK2) | Uniprot |
Research Backgrounds
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Hyperphosphorylated during the G1/S phase transition.
Nucleus>Nucleolus.
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
Binds PAWR, POLR2J, RB1/RB, RBL1/P107, RBL2/P130, and SP1. May also bind MAPT.
Belongs to the AATF family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.