Product: AATF Antibody
Catalog: DF3453
Description: Rabbit polyclonal antibody to AATF
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 63 KD; 63kD(Calculated).
Uniprot: Q9NY61
RRID: AB_2835819

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Rabbit(91%), Dog(91%)
Clonality:
Polyclonal
Specificity:
AATF Antibody detects endogenous levels of total AATF.
RRID:
AB_2835819
Cite Format: Affinity Biosciences Cat# DF3453, RRID:AB_2835819.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AATF; AATF_HUMAN; Apoptosis antagonizing transcription factor; Apoptosis-antagonizing transcription factor; BFR2; CHE 1; CHE1; DED; Protein AATF; Rb binding protein Che 1; Rb-binding protein Che-1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9NY61 AATF_HUMAN:

Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.

Sequence:
MAGPQPLALQLEQLLNPRPSEADPEADPEEATAARVIDRFDEGEDGEGDFLVVGSIRKLASASLLDTDKRYCGKTTSRKAWNEDHWEQTLPGSSDEEISDEEGSGDEDSEGLGLEEYDEDDLGAAEEQECGDHRESKKSRSHSAKTPGFSVQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDDAEDSQGESEEDRAGDRNSEDDGVVMTFSSVKVSEEVEKGRAVKNQIALWDQLLEGRIKLQKALLTTNQLPQPDVFPLFKDKGGPEFSSALKNSHKALKALLRSLVGLQEELLFQYPDTRYLVDGTKPNAGSEEISSEDDELVEEKKQQRRRVPAKRKLEMEDYPSFMAKRFADFTVYRNRTLQKWHDKTKLASGKLGKGFGAFERSILTQIDHILMDKERLLRRTQTKRSVYRVLGKPEPAAQPVPESLPGEPEILPQAPANAHLKDLDEEIFDDDDFYHQLLRELIERKTSSLDPNDQVAMGRQWLAIQKLRSKIHKKVDRKASKGRKLRFHVLSKLLSFMAPIDHTTMNDDARTELYRSLFGQLHPPDEGHGD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Dog
91
Rabbit
91
Chicken
64
Xenopus
55
Zebrafish
55
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NY61 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S20 Phosphorylation
S55 Phosphorylation
K58 Sumoylation
K58 Ubiquitination
S61 Phosphorylation
S63 Phosphorylation
T67 Phosphorylation
K69 Ubiquitination
K74 Ubiquitination
S93 Phosphorylation
S94 Phosphorylation
S99 Phosphorylation
S104 Phosphorylation
S109 Phosphorylation
Y117 Phosphorylation
S141 Phosphorylation O96017 (CHEK2)
S143 Phosphorylation O96017 (CHEK2)
K145 Ubiquitination
T146 Phosphorylation
S150 Phosphorylation
S153 Phosphorylation
S155 Phosphorylation
K159 Ubiquitination
S189 Phosphorylation Q13315 (ATM)
S203 Phosphorylation
S213 Phosphorylation
S218 Phosphorylation
K223 Sumoylation
K228 Ubiquitination
K246 Ubiquitination
T251 Phosphorylation
K264 Ubiquitination
K266 Ubiquitination
S273 Phosphorylation
K276 Methylation
K276 Ubiquitination
S288 Phosphorylation
Y305 Phosphorylation
T310 Phosphorylation
S316 Phosphorylation
S320 Phosphorylation
S321 Phosphorylation
K342 Sumoylation
K342 Ubiquitination
K354 Ubiquitination
T366 Phosphorylation P49137 (MAPKAPK2)
K369 Acetylation
K380 Acetylation
K383 Acetylation
K383 Ubiquitination
S391 Phosphorylation
K403 Ubiquitination
T410 Phosphorylation
T412 Phosphorylation
K422 Sumoylation
K422 Ubiquitination
K451 Ubiquitination
K475 Sumoylation
K475 Ubiquitination
T476 Phosphorylation
S477 Phosphorylation O96017 (CHEK2)
K496 Ubiquitination
S510 Phosphorylation O96017 (CHEK2)

Research Backgrounds

Function:

May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.

PTMs:

Hyperphosphorylated during the G1/S phase transition.

Subcellular Location:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.

Subunit Structure:

Binds PAWR, POLR2J, RB1/RB, RBL1/P107, RBL2/P130, and SP1. May also bind MAPT.

Family&Domains:

Belongs to the AATF family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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