MBD3 Antibody - #AF0353

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Product: MBD3 Antibody
Catalog: AF0353
Description: Rabbit polyclonal antibody to MBD3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 33kDa; 33kD(Calculated).
Uniprot: O95983
RRID: AB_2833519

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
MBD3 Antibody detects endogenous levels of total MBD3.
RRID:
AB_2833519
Cite Format: Affinity Biosciences Cat# AF0353, RRID:AB_2833519.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI181826; AU019209; MBD 3; Mbd3; MBD3: methyl CpG binding domain protein 3; MBD3_HUMAN; Methyl CpG binding domain protein 3; Methyl CpG binding protein MBD3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3;

Immunogens

Immunogen:

A synthesized peptide derived from human MBD3, corresponding to a region within C-terminal amino acids.

Uniprot:

O95983(MBD3_HUMAN) >>Visit HPA database.

Gene(ID):
MBD3(53615)
Description:
MBD3 methyl-CpG binding (MBD) proteins appear to bind differentially methylated domains (DMDs) of DNA, to recruit repression complexes, and to silence the locus. The methyl CpG binding domain of human MBD3 may not directly bind mCpG but does interact with the NuRD/Mi2 components HDAC1 and MTA2. Plays a role in histone deacetylation and nucleosome remodeling. Increased levels have been reported in lung cancer and glioblastomas and may be a therapeutic target.
Sequence:
MERKRWECPALPQGWEREEVPRRSGLSAGHRDVFYYSPSGKKFRSKPQLARYLGGSMDLSTFDFRTGKMLMSKMNKSRQRVRYDSSNQVKGKPDLNTALPVRQTASIFKQPVTKITNHPSNKVKSDPQKAVDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDETLLSAIASALHTSTMPITGQLSAAVEKNPGVWLNTTQPLCKAFMVTDEDIRKQEELVQQVRKRLEEALMADMLAHVEELARDGEAPLDKACAEDDDEEDEEEEEEEPDPDPEMEHV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
91
Xenopus
64
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95983 As Substrate

Site PTM Type Enzyme
S24 Phosphorylation O14965 (AURKA)
Y35 Phosphorylation
Y36 Phosphorylation
S37 Phosphorylation
S39 Phosphorylation
S45 Phosphorylation
S56 Phosphorylation
T61 Phosphorylation
T66 Phosphorylation
S77 Phosphorylation
Y83 Phosphorylation
S85 Phosphorylation O14965 (AURKA)
S86 Phosphorylation
K92 Ubiquitination
T97 Phosphorylation
K109 Acetylation
K109 Sumoylation
K129 Ubiquitination
K141 Acetylation
K141 Ubiquitination
K142 Ubiquitination
S144 Phosphorylation
K157 Ubiquitination
T221 Phosphorylation
K227 Ubiquitination

Research Backgrounds

Function:

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself. Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides. Recruits histone deacetylases and DNA methyltransferases.

Subcellular Location:

Nucleus. Chromosome.
Note: Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer with MBD2. Part of the NuRD and the MeCP1 complex. Interacts with BCL6, HDAC1, MTA2, DNMT1, p66-alpha and p66-beta.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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