ZC3H7B Antibody - #DF3465

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Product: ZC3H7B Antibody
Catalog: DF3465
Description: Rabbit polyclonal antibody to ZC3H7B
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 111 KD; 110kD(Calculated).
Uniprot: Q9UGR2
RRID: AB_2835829

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
ZC3H7B Antibody detects endogenous levels of total ZC3H7B.
RRID:
AB_2835829
Cite Format: Affinity Biosciences Cat# DF3465, RRID:AB_2835829.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp434K0920; FLJ13787; KIAA1031; Rotavirus 'X' associated non structural protein; Rotavirus X protein associated with NSP3; RoXaN; Ubiquitous tetratricopeptide containing protein RoXaN; Zinc finger CCCH domain containing protein 7B; Zinc finger CCCH type containing 7B;

Immunogens

Immunogen:
Uniprot:

Q9UGR2(Z3H7B_HUMAN) >>Visit HPA database.

Gene(ID):
ZC3H7B(23264)
Sequence:
MERQKRKADIEKGLQFIQSTLPLKQEEYEAFLLKLVQNLFAEGNDLFREKDYKQALVQYMEGLNVADYAASDQVALPRELLCKLHVNRAACYFTMGLYEKALEDSEKALGLDSESIRALFRKARALNELGRHKEAYECSSRCSLALPHDESVTQLGQELAQKLGLRVRKAYKRPQELETFSLLSNGTAAGVADQGTSNGLGSIDDIETDCYVDPRGSPALLPSTPTMPLFPHVLDLLAPLDSSRTLPSTDSLDDFSDGDVFGPELDTLLDSLSLVQGGLSGSGVPSELPQLIPVFPGGTPLLPPVVGGSIPVSSPLPPASFGLVMDPSKKLAASVLDALDPPGPTLDPLDLLPYSETRLDALDSFGSTRGSLDKPDSFMEETNSQDHRPPSGAQKPAPSPEPCMPNTALLIKNPLAATHEFKQACQLCYPKTGPRAGDYTYREGLEHKCKRDILLGRLRSSEDQTWKRIRPRPTKTSFVGSYYLCKDMINKQDCKYGDNCTFAYHQEEIDVWTEERKGTLNRDLLFDPLGGVKRGSLTIAKLLKEHQGIFTFLCEICFDSKPRIISKGTKDSPSVCSNLAAKHSFYNNKCLVHIVRSTSLKYSKIRQFQEHFQFDVCRHEVRYGCLREDSCHFAHSFIELKVWLLQQYSGMTHEDIVQESKKYWQQMEAHAGKASSSMGAPRTHGPSTFDLQMKFVCGQCWRNGQVVEPDKDLKYCSAKARHCWTKERRVLLVMSKAKRKWVSVRPLPSIRNFPQQYDLCIHAQNGRKCQYVGNCSFAHSPEERDMWTFMKENKILDMQQTYDMWLKKHNPGKPGEGTPISSREGEKQIQMPTDYADIMMGYHCWLCGKNSNSKKQWQQHIQSEKHKEKVFTSDSDASGWAFRFPMGEFRLCDRLQKGKACPDGDKCRCAHGQEELNEWLDRREVLKQKLAKARKDMLLCPRDDDFGKYNFLLQEDGDLAGATPEAPAAAATATTGE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Xenopus
82
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UGR2 As Substrate

Site PTM Type Enzyme
K24 Ubiquitination
K53 Ubiquitination
K83 Ubiquitination
K107 Ubiquitination
K133 Ubiquitination
S217 Phosphorylation
S223 Phosphorylation
T224 Phosphorylation
T226 Phosphorylation
K330 Ubiquitination
S364 Phosphorylation
S367 Phosphorylation
S371 Phosphorylation
S399 Phosphorylation
K422 Ubiquitination
Y429 Phosphorylation
K431 Ubiquitination
Y439 Phosphorylation
Y441 Phosphorylation
K448 Ubiquitination
T476 Phosphorylation
S477 Phosphorylation
K533 Ubiquitination
S536 Phosphorylation
S572 Phosphorylation
K582 Ubiquitination
K589 Ubiquitination
Y648 Phosphorylation
T652 Phosphorylation
K661 Ubiquitination
K662 Ubiquitination
K673 Ubiquitination
S675 Phosphorylation
S677 Phosphorylation
K694 Ubiquitination
K711 Ubiquitination
K714 Ubiquitination
K719 Ubiquitination
S735 Phosphorylation
K768 Ubiquitination
K791 Ubiquitination
K807 Ubiquitination
K808 Ubiquitination
K813 Ubiquitination
T818 Phosphorylation
K855 Ubiquitination
K865 Ubiquitination
K869 Ubiquitination
K935 Ubiquitination
K948 Ubiquitination

Research Backgrounds

Function:

May be a specific regulator of miRNA biogenesis. Binds to microRNAs MIR7-1, MIR16-2 and MIR29A hairpins recognizing the 'ATA(A/T)' motif in the apical loop.

Subcellular Location:

Nucleus.
Note: Nuclear localization seems to be depleted upon rotavirus A infection.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

(Microbial infection) Interacts (via LD motif) with rotavirus A NSP3 (via the coiled-coil region).

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