TRPS1 Antibody - #DF3467

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Product: TRPS1 Antibody
Catalog: DF3467
Description: Rabbit polyclonal antibody to TRPS1
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 142 KD; 142kD(Calculated).
Uniprot: Q9UHF7
RRID: AB_2835831

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(82%), Dog(91%)
Clonality:
Polyclonal
Specificity:
TRPS1 Antibody detects endogenous levels of total TRPS1.
RRID:
AB_2835831
Cite Format: Affinity Biosciences Cat# DF3467, RRID:AB_2835831.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GC79; LGCR; Transcriptional repressor GATA binding 1; Tricho rhino phalangeal syndrome I; Tricho-rhino-phalangeal syndrome type I protein; Trichorhinophalangeal syndrome I; Trichorhinophalangeal syndrome I homolog; TRPS 1; trpS1; TRPS1 gene; TRPS1_HUMAN; Zinc finger protein GC79; Zinc finger transcription factor TRPS 1; Zinc finger transcription factor Trps1;

Immunogens

Immunogen:
Uniprot:

Q9UHF7(TRPS1_HUMAN) >>Visit HPA database.

Gene(ID):
TRPS1(7227)
Expression:
Q9UHF7 TRPS1_HUMAN:

Ubiquitously expressed in the adult. Found in fetal brain, lung, kidney, liver, spleen and thymus. More highly expressed in androgen-dependent than in androgen-independent prostate cancer cells.

Sequence:
MVRKKNPPLRNVASEGEGQILEPIGTESKVSGKNKEFSADQMSENTDQSDAAELNHKEEHSLHVQDPSSSSKKDLKSAVLSEKAGFNYESPSKGGNFPSFPHDEVTDRNMLAFSSPAAGGVCEPLKSPQRAEADDPQDMACTPSGDSLETKEDQKMSPKATEETGQAQSGQANCQGLSPVSVASKNPQVPSDGGVRLNKSKTDLLVNDNPDPAPLSPELQDFKCNICGYGYYGNDPTDLIKHFRKYHLGLHNRTRQDAELDSKILALHNMVQFSHSKDFQKVNRSVFSGVLQDINSSRPVLLNGTYDVQVTSGGTFIGIGRKTPDCQGNTKYFRCKFCNFTYMGNSSTELEQHFLQTHPNKIKASLPSSEVAKPSEKNSNKSIPALQSSDSGDLGKWQDKITVKAGDDTPVGYSVPIKPLDSSRQNGTEATSYYWCKFCSFSCESSSSLKLLEHYGKQHGAVQSGGLNPELNDKLSRGSVINQNDLAKSSEGETMTKTDKSSSGAKKKDFSSKGAEDNMVTSYNCQFCDFRYSKSHGPDVIVVGPLLRHYQQLHNIHKCTIKHCPFCPRGLCSPEKHLGEITYPFACRKSNCSHCALLLLHLSPGAAGSSRVKHQCHQCSFTTPDVDVLLFHYESVHESQASDVKQEANHLQGSDGQQSVKESKEHSCTKCDFITQVEEEISRHYRRAHSCYKCRQCSFTAADTQSLLEHFNTVHCQEQDITTANGEEDGHAISTIKEEPKIDFRVYNLLTPDSKMGEPVSESVVKREKLEEKDGLKEKVWTESSSDDLRNVTWRGADILRGSPSYTQASLGLLTPVSGTQEQTKTLRDSPNVEAAHLARPIYGLAVETKGFLQGAPAGGEKSGALPQQYPASGENKSKDESQSLLRRRRGSGVFCANCLTTKTSLWRKNANGGYVCNACGLYQKLHSTPRPLNIIKQNNGEQIIRRRTRKRLNPEALQAEQLNKQQRGSNEEQVNGSPLERRSEDHLTESHQREIPLPSLSKYEAQGSLTKSHSAQQPVLVSQTLDIHKRMQPLHIQIKSPQESTGDPGNSSSVSEGKGSSERGSPIEKYMRPAKHPNYSPPGSPIEKYQYPLFGLPFVHNDFQSEADWLRFWSKYKLSVPGNPHYLSHVPGLPNPCQNYVPYPTFNLPPHFSAVGSDNDIPLDLAIKHSRPGPTANGASKEKTKAPPNVKNEGPLNVVKTEKVDRSTQDELSTKCVHCGIVFLDEVMYALHMSCHGDSGPFQCSICQHLCTDKYDFTTHIQRGLHRNNAQVEKNGKPKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Rabbit
82
Xenopus
64
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UHF7 As Substrate

Site PTM Type Enzyme
S14 Phosphorylation
S77 Phosphorylation
S90 Phosphorylation
S92 Phosphorylation
S99 Phosphorylation
S115 Phosphorylation
S127 Phosphorylation
T142 Phosphorylation
S157 Phosphorylation
K159 Methylation
S178 Phosphorylation
S181 Phosphorylation
S184 Phosphorylation
S216 Phosphorylation
S276 Phosphorylation
S369 Phosphorylation
S391 Phosphorylation
K404 Ubiquitination
K418 Ubiquitination
S479 Phosphorylation
S573 Phosphorylation
S603 Phosphorylation
S654 Phosphorylation
S682 Phosphorylation
T751 Phosphorylation
K766 Sumoylation
S786 Phosphorylation
S803 Phosphorylation
S805 Phosphorylation
Y806 Phosphorylation
T807 Phosphorylation
T815 Phosphorylation
S818 Phosphorylation
T826 Phosphorylation
S830 Phosphorylation
K850 Sumoylation
S873 Phosphorylation
S892 Phosphorylation
S928 Phosphorylation
T929 Phosphorylation
S970 Phosphorylation
S978 Phosphorylation
S984 Phosphorylation
K1003 Sumoylation
S1009 Phosphorylation
S1013 Phosphorylation
S1041 Phosphorylation
S1054 Phosphorylation
S1056 Phosphorylation
S1061 Phosphorylation
S1062 Phosphorylation
S1066 Phosphorylation
Y1080 Phosphorylation
S1081 Phosphorylation
S1085 Phosphorylation
K1192 Sumoylation
K1201 Sumoylation
T1202 Phosphorylation
S1208 Phosphorylation
T1209 Phosphorylation
S1214 Phosphorylation
T1215 Phosphorylation

Research Backgrounds

Function:

Transcriptional repressor. Binds specifically to GATA sequences and represses expression of GATA-regulated genes at selected sites and stages in vertebrate development. Regulates chondrocyte proliferation and differentiation. Executes multiple functions in proliferating chondrocytes, expanding the region of distal chondrocytes, activating proliferation in columnar cells and supporting the differentiation of columnar into hypertrophic chondrocytes.

PTMs:

Sumoylated. Sumoylation in the repressor domain inhibits the transcription repression activity. Sumoylation on Lys-1201 is the major site. Appears to be sumoylated on multiple sites.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed in the adult. Found in fetal brain, lung, kidney, liver, spleen and thymus. More highly expressed in androgen-dependent than in androgen-independent prostate cancer cells.

Subunit Structure:

Interacts with RNF4; regulates TRPS1 repressor activity. Interacts specifically with the activator form of GLI3 (GLI3A) but not with the repressor form (GLI3R).

Restrictive clause

 

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