ADD2 Antibody - #DF3509

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Product: ADD2 Antibody
Catalog: DF3509
Description: Rabbit polyclonal antibody to ADD2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 81 KD; 81kD(Calculated).
Uniprot: P35612
RRID: AB_2835871

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
ADD2 Antibody detects endogenous levels of total ADD2.
RRID:
AB_2835871
Cite Format: Affinity Biosciences Cat# DF3509, RRID:AB_2835871.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADD1; ADD2; ADD3; ADDA; ADDB; ADDL; Adducin 1 (alpha); Adducin 1; Adducin 2 (beta); Adducin 2; Adducin 3 (gamma); Adducin 3; Adducin like; Alpha adducin; Beta adducin;

Immunogens

Immunogen:
Uniprot:

P35612(ADDB_HUMAN) >>Visit HPA database.

Gene(ID):
ADD2(119)
Expression:
P35612 ADDB_HUMAN:

Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line.

Sequence:
MSEETVPEAASPPPPQGQPYFDRFSEDDPEYMRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPTSSMNVSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFCLHSAIYAARPDVRCIIHLHTPATAAVSAMKWGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGVVALGDTVEEAFYKIFHLQAACEIQVSALSSAGGVENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGAPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRSMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLEMRNKIREQNRQDVKSAGPQSQLLASVIAEKSRSPSTESQLMSKGDEDTKDDSEETVPNPFSQLTDQELEEYKKEVERKKLELDGEKETAPEEPGSPAKSAPASPVQSPAKEAETKSPLVSPSKSLEEGTKKTETSKAATTEPETTQPEGVVVNGREEEQTAEEILSKGLSQMTTSADTDVDTSKDKTESVTSGPMSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35612 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
T5 Phosphorylation
S11 Phosphorylation
Y31 Phosphorylation
T55 Phosphorylation
S60 Phosphorylation
S62 Phosphorylation
Y377 Phosphorylation
S396 Phosphorylation
Y440 Phosphorylation
S455 Phosphorylation
K464 Acetylation
S472 Phosphorylation
Y489 Phosphorylation P06241 (FYN)
S512 Phosphorylation
S522 Phosphorylation
S528 Phosphorylation
S530 Phosphorylation
S532 Phosphorylation
T533 Phosphorylation
S535 Phosphorylation
K576 Acetylation
K583 Acetylation
T585 Phosphorylation
S592 Phosphorylation
K595 Acetylation
S596 Phosphorylation
S600 Phosphorylation
S604 Phosphorylation Q00535 (CDK5)
T611 Phosphorylation
S613 Phosphorylation P49841 (GSK3B)
S617 Phosphorylation Q00535 (CDK5)
S619 Phosphorylation
S621 Phosphorylation
T626 Phosphorylation
T631 Phosphorylation
S632 Phosphorylation
T675 Phosphorylation
K681 Acetylation
K683 Acetylation
T684 Phosphorylation
S693 Phosphorylation P49841 (GSK3B)
S697 Phosphorylation
S699 Phosphorylation
S701 Phosphorylation Q00535 (CDK5)
S703 Phosphorylation
T711 Phosphorylation
S713 Phosphorylation O14757 (CHEK1) , P17612 (PRKACA) , Q05513 (PRKCZ) , P17252 (PRKCA)
S718 Phosphorylation
S726 Phosphorylation P17252 (PRKCA)

Research Backgrounds

Function:

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to the erythrocyte membrane receptor SLC2A1/GLUT1 and may therefore provide a link between the spectrin cytoskeleton to the plasma membrane. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit.

PTMs:

The N-terminus is blocked.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line.

Subunit Structure:

Heterodimer of an alpha and a beta subunit. Found in a complex with ADD2, DMTN and SLC2A1. Interacts with SLC2A1.

Family&Domains:

Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Belongs to the aldolase class II family. Adducin subfamily.

Restrictive clause

 

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