Product: ADD3 Antibody
Catalog: DF3510
Description: Rabbit polyclonal antibody to ADD3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 75 KD; 79kD(Calculated).
Uniprot: Q9UEY8
RRID: AB_2835872

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ADD3 Antibody detects endogenous levels of total ADD3.
RRID:
AB_2835872
Cite Format: Affinity Biosciences Cat# DF3510, RRID:AB_2835872.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADD3; ADDG_HUMAN; ADDL; Adducin 3 (gamma); Adducin 3; Adducin, gamma; Adducin-like; Adducin-like protein 70; Gamma-adducin;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9UEY8 ADDG_HUMAN:

Isoform 1 is ubiquitously expressed.

Sequence:
MSSDASQGVITTPPPPSMPHKERYFDRINENDPEYIRERNMSPDLRQDFNMMEQRKRVTQILQSPAFREDLECLIQEQMKKGHNPTGLLALQQIADYIMANSFSGFSSPPLSLGMVTPINDLPGADTSSYVKGEKLTRCKLASLYRLVDLFGWAHLANTYISVRISKEQDHIIIIPRGLSFSEATASNLVKVNIIGEVVDQGSTNLKIDHTGFSPHAAIYSTRPDVKCVIHIHTLATAAVSSMKCGILPISQESLLLGDVAYYDYQGSLEEQEERIQLQKVLGPSCKVLVLRNHGVVALGETLEEAFHYIFNVQLACEIQVQALAGAGGVDNLHVLDFQKYKAFTYTVAASGGGGVNMGSHQKWKVGEIEFEGLMRTLDNLGYRTGYAYRHPLIREKPRHKSDVEIPATVTAFSFEDDTVPLSPLKYMAQRQQREKTRWLNSPNTYMKVNVPEESRNGETSPRTKITWMKAEDSSKVSGGTPIKIEDPNQFVPLNTNPNEVLEKRNKIREQNRYDLKTAGPQSQLLAGIVVDKPPSTMQFEDDDHGPPAPPNPFSHLTEGELEEYKRTIERKQQGLEDAEQELLSDDASSVSQIQSQTQSPQNVPEKLEENHELFSKSFISMEVPVMVVNGKDDMHDVEDELAKRVSRLSTSTTIENIEITIKSPEKIEEVLSPEGSPSKSPSKKKKKFRTPSFLKKNKKKEKVEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
78
Xenopus
67
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UEY8 As Substrate

Site PTM Type Enzyme
S2 Acetylation
T12 Phosphorylation
S17 Phosphorylation
K21 Ubiquitination
R27 Methylation
Y35 Phosphorylation
S42 Phosphorylation
T59 Phosphorylation
S64 Phosphorylation
K280 Ubiquitination
K340 Methylation
S360 Phosphorylation
Y383 Phosphorylation
Y389 Phosphorylation
R390 Methylation
S402 Phosphorylation
T409 Phosphorylation
T411 Phosphorylation
S414 Phosphorylation
T419 Phosphorylation
S423 Phosphorylation P24941 (CDK2)
K426 Ubiquitination
Y427 Phosphorylation
K436 Acetylation
S442 Phosphorylation
T445 Phosphorylation
Y446 Phosphorylation
K448 Ubiquitination
S455 Phosphorylation
T460 Phosphorylation
S461 Phosphorylation
T464 Phosphorylation
T467 Phosphorylation
S478 Phosphorylation
T481 Phosphorylation
K504 Ubiquitination
Y565 Phosphorylation
S618 Phosphorylation
S621 Phosphorylation
S647 Phosphorylation
S650 Phosphorylation
T651 Phosphorylation
S652 Phosphorylation
T654 Phosphorylation
S673 Phosphorylation
S677 Phosphorylation
S679 Phosphorylation
S681 Phosphorylation
S683 Phosphorylation
T691 Phosphorylation
S693 Phosphorylation O14757 (CHEK1)

Research Backgrounds

Function:

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Plays a role in actin filament capping. Binds to calmodulin.

PTMs:

Sumoylated.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is ubiquitously expressed.

Subunit Structure:

Heterodimer of an alpha and a gamma subunit.

Family&Domains:

Comprised of three regions: a N-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Belongs to the aldolase class II family. Adducin subfamily.

Restrictive clause

 

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