API-5 Antibody - #DF3515

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Product: API-5 Antibody
Catalog: DF3515
Description: Rabbit polyclonal antibody to API-5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Sheep, Dog
Mol.Wt.: 58 KD; 59kD(Calculated).
Uniprot: Q9BZZ5
RRID: AB_2835877

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(89%), Horse(100%), Sheep(89%), Dog(89%)
Clonality:
Polyclonal
Specificity:
API-5 Antibody detects endogenous levels of total API-5.
RRID:
AB_2835877
Cite Format: Affinity Biosciences Cat# DF3515, RRID:AB_2835877.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AAC 11; AAC-11; AAC11; Antiapoptosis clone 11 protein; API 5; API-5; api5; API5 like 1; API5_HUMAN; API5L1; Apoptosis inhibitor 5; Cell migration inducing gene 8 protein; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2 interacting factor 2; Fibroblast growth factor 2-interacting factor; FIF; MIG 8; MIG8; Migration inducing protein MIG8; Protein XAGL;

Immunogens

Immunogen:
Uniprot:

Q9BZZ5(API5_HUMAN) >>Visit HPA database.

Gene(ID):
API5(8539)
Expression:
Q9BZZ5 API5_HUMAN:

Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.

Sequence:
MPTVEELYRNYGILADATEQVGQHKDAYQVILDGVKGGTKEKRLAAQFIPKFFKHFPELADSAINAQLDLCEDEDVSIRRQAIKELPQFATGENLPRVADILTQLLQTDDSAEFNLVNNALLSIFKMDAKGTLGGLFSQILQGEDIVRERAIKFLSTKLKTLPDEVLTKEVEELILTESKKVLEDVTGEEFVLFMKILSGLKSLQTVSGRQQLVELVAEQADLEQTFNPSDPDCVDRLLQCTRQAVPLFSKNVHSTRFVTYFCEQVLPNLGTLTTPVEGLDIQLEVLKLLAEMSSFCGDMEKLETNLRKLFDKLLEYMPLPPEEAENGENAGNEEPKLQFSYVECLLYSFHQLGRKLPDFLTAKLNAEKLKDFKIRLQYFARGLQVYIRQLRLALQGKTGEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWKPVQKVEIGQKRASEDTTSGSPPKKSSAGPKRDARQIYNPPSGKYSSNLGNFNYEQRGAFRGSRGGRGWGTRGNRSRGRLY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
89
Sheep
89
Dog
89
Bovine
78
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BZZ5 As Substrate

Site PTM Type Enzyme
T18 Phosphorylation
Y28 Phosphorylation
K36 Ubiquitination
K51 Acetylation
K51 Ubiquitination
K84 Ubiquitination
K153 Ubiquitination
K160 Ubiquitination
K169 Ubiquitination
K180 Ubiquitination
K202 Ubiquitination
K251 Acetylation
K369 Ubiquitination
T399 Phosphorylation
K448 Acetylation
K454 Acetylation
K454 Ubiquitination
S457 Phosphorylation
T460 Phosphorylation
T461 Phosphorylation
S462 Phosphorylation
S464 Phosphorylation
S470 Phosphorylation
Y481 Phosphorylation
S485 Phosphorylation
K487 Methylation
R500 Methylation
R504 Methylation
R507 Methylation
R510 Methylation

Research Backgrounds

Function:

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.

PTMs:

Acetylation at Lys-251 impairs antiapoptotic function.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Mainly nuclear. Can also be cytoplasmic.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.

Subunit Structure:

Monomer. Interacts with FGF2 and ACIN1.

Family&Domains:

Two regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2.

Belongs to the API5 family.

Restrictive clause

 

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