CAPN12 Antibody - #DF3868
(2)
| Product: | CAPN12 Antibody |
| Catalog: | DF3868 |
| Description: | Rabbit polyclonal antibody to CAPN12 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Sheep, Dog |
| Mol.Wt.: | 80 KD; 81kD(Calculated). |
| Uniprot: | Q6ZSI9 |
| RRID: | AB_2835906 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(82%), Bovine(91%), Sheep(82%), Dog(82%)
Clonality:
Polyclonal
Specificity:
CAPN12 Antibody detects endogenous levels of total CAPN12.
RRID:
AB_2835906
Cite Format: Affinity Biosciences Cat# DF3868, RRID:AB_2835906.
Cite Format: Affinity Biosciences Cat# DF3868, RRID:AB_2835906.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Calcium-activated neutral proteinase 12; Calpain-12; Calpain12; CAN12_HUMAN; CANP 12; CAPN 12; CAPN12; EC 3.4.22.; MGC20576;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q6ZSI9 CAN12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASSSGRVTIQLVDEEAGVGAGRLQLFRGQSYEAIRAACLDSGILFRDPYFPAGPDALGYDQLGPDSEKAKGVKWMRPHEFCAEPKFICEDMSRTDVCQGSLGNCWFLAAAASLTLYPRLLRRVVPPGQDFQHGYAGVFHFQLWQFGRWMDVVVDDRLPVREGKLMFVRSEQRNEFWAPLLEKAYAKLHGSYEVMRGGHMNEAFVDFTGGVGEVLYLRQNSMGLFSALRHALAKESLVGATALSDRGEYRTEEGLVKGHAYSITGTHKVFLGFTKVRLLRLRNPWGCVEWTGAWSDSCPRWDTLPTECRDALLVKKEDGEFWMELRDFLLHFDTVQICSLSPEVLGPSPEGGGWHVHTFQGRWVRGFNSGGSQPNAETFWTNPQFRLTLLEPDEEDDEDEEGPWGGWGAAGARGPARGGRTPKCTVLLSLIQRNRRRLRAKGLTYLTVGFHVFQIPEELLGLWDSPRSHALLPRLLRADRSPLSARRDVTRRCCLRPGHYLVVPSTAHAGDEADFTLRVFSERRHTAVEIDDVISADLQSLQGPYLPLELGLEQLFQELAGEEEELNASQLQALLSIALEPARAHTSTPREIGLRTCEQLLQCFGHGQSLALHHFQQLWGYLLEWQAIFNKFDEDTSGTMNSYELRLALNAAGFHLNNQLTQTLTSRYRDSRLRVDFERFVSCVAHLTCIFCHCSQHLDGGEGVICLTHRQWMEVATFS
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Bovine
91
Pig
82
Sheep
82
Dog
82
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q6ZSI9 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y249 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| T661 | Phosphorylation | Uniprot | |
| Y668 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Calcium-regulated non-lysosomal thiol-protease.
Family&Domains:
Belongs to the peptidase C2 family.
Restrictive clause
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For Research Use Only.
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