CNN2 Antibody - #DF3870
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| Product: | CNN2 Antibody |
| Catalog: | DF3870 |
| Description: | Rabbit polyclonal antibody to CNN2 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Sheep, Dog, Chicken, Xenopus |
| Mol.Wt.: | 33 KD; 34kD(Calculated). |
| Uniprot: | Q99439 |
| RRID: | AB_2835908 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(82%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
CNN2 Antibody detects endogenous levels of total CNN2.
RRID:
AB_2835908
Cite Format: Affinity Biosciences Cat# DF3870, RRID:AB_2835908.
Cite Format: Affinity Biosciences Cat# DF3870, RRID:AB_2835908.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Calponin 2; Calponin H2; Calponin H2, smooth muscle; Calponin-2; Cnn2; CNN2_HUMAN; Neutral calponin; smooth muscle;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q99439 CNN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSTQFNKGPSYGLSAEVKNRLLSKYDPQKEAELRTWIEGLTGLSIGPDFQKGLKDGTILCTLMNKLQPGSVPKINRSMQNWHQLENLSNFIKAMVSYGMNPVDLFEANDLFESGNMTQVQVSLLALAGKAKTKGLQSGVDIGVKYSEKQERNFDDATMKAGQCVIGLQMGTNKCASQSGMTAYGTRRHLYDPKNHILPPMDHSTISLQMGTNKCASQVGMTAPGTRRHIYDTKLGTDKCDNSSMSLQMGYTQGANQSGQVFGLGRQIYDPKYCPQGTVADGAPSGTGDCPDPGEVPEYPPYYQEEAGY
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Pig
91
Xenopus
91
Chicken
82
Zebrafish
73
Horse
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99439 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| K8 | Acetylation | Uniprot | |
| K8 | Methylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| Y12 | Phosphorylation | P12931 (SRC) | Uniprot |
| S15 | Phosphorylation | Uniprot | |
| K19 | Sumoylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K25 | Acetylation | Uniprot | |
| K25 | Ubiquitination | Uniprot | |
| Y26 | Phosphorylation | Uniprot | |
| K30 | Acetylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| T42 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| C61 | S-Nitrosylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| K160 | Methylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| C164 | S-Nitrosylation | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| K174 | Methylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| Y184 | Phosphorylation | Uniprot | |
| T186 | Phosphorylation | Uniprot | |
| R187 | Methylation | Uniprot | |
| R188 | Methylation | Uniprot | |
| Y191 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| C215 | S-Nitrosylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| T222 | Phosphorylation | Uniprot | |
| T226 | Phosphorylation | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| T233 | Phosphorylation | Uniprot | |
| K234 | Sumoylation | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| Y251 | Phosphorylation | Uniprot | |
| T252 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| Y269 | Phosphorylation | Uniprot | |
| T278 | Phosphorylation | Uniprot | |
| Y299 | Phosphorylation | Uniprot | |
| Y302 | Phosphorylation | Uniprot | |
| Y303 | Phosphorylation | Uniprot | |
| Y309 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue Specificity:
Heart and smooth muscle.
Family&Domains:
Belongs to the calponin family.
References
1). Knockdown of CNN3 Impairs Myoblast Proliferation, Differentiation, and Protein Synthesis via the mTOR Pathway. Frontiers in Physiology, 2021
(PubMed: 34305633)
[IF=4.0]
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