CDCA3 Antibody - #DF3544

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Product: CDCA3 Antibody
Catalog: DF3544
Description: Rabbit polyclonal antibody to CDCA3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 28 KD; 29kD(Calculated).
Uniprot: Q99618
RRID: AB_2835917

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(82%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(82%)
Clonality:
Polyclonal
Specificity:
CDCA3 Antibody detects endogenous levels of total CDCA3.
RRID:
AB_2835917
Cite Format: Affinity Biosciences Cat# DF3544, RRID:AB_2835917.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C8; CDCA 3; Cell division cycle associated 3; Cell division cycle associated protein 3; Gene rich cluster C8; Gene rich cluster protein C8; GRCC 8; GRCC8; MGC2577; TOME 1; TOME1; Trigger of mitotic entry 1; Trigger of mitotic entry protein 1;

Immunogens

Immunogen:
Uniprot:

Q99618(CDCA3_HUMAN) >>Visit HPA database.

Gene(ID):
CDCA3(83461)
Sequence:
MGSAKSVPVTPARPPPHNKHLARVADPRSPSAGILRTPIQVESSPQPGLPAGEQLEGLKHAQDSDPRSPTLGIARTPMKTSSGDPPSPLVKQLSEVFETEDSKSNLPPEPVLPPEAPLSSELDLPLGTQLSVEEQMPPWNQTEFPSKQVFSKEEARQPTETPVASQSSDKPSRDPETPRSSGSMRNRWKPNSSKVLGRSPLTILQDDNSPGTLTLRQGKRPSPLSENVSELKEGAILGTGRLLKTGGRAWEQGQDHDKENQHFPLVES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Sheep
91
Bovine
82
Dog
82
Chicken
75
Pig
73
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99618 As Substrate

Site PTM Type Enzyme
T10 Phosphorylation
R28 Methylation
S29 Phosphorylation
S31 Phosphorylation
R36 Methylation
T37 Phosphorylation
S43 Phosphorylation
S44 Phosphorylation
K59 Ubiquitination
S64 Phosphorylation
S68 Phosphorylation
T70 Phosphorylation
T76 Phosphorylation
K79 Methylation
S82 Phosphorylation
S87 Phosphorylation
S94 Phosphorylation
T99 Phosphorylation
S102 Phosphorylation
T159 Phosphorylation
T161 Phosphorylation
S165 Phosphorylation
S167 Phosphorylation
K170 Ubiquitination
T177 Phosphorylation
S181 Phosphorylation
K189 Ubiquitination
S199 Phosphorylation
T202 Phosphorylation
S209 Phosphorylation
T212 Phosphorylation
T214 Phosphorylation
K219 Ubiquitination
S222 Phosphorylation
S229 Phosphorylation
K232 Ubiquitination
T239 Phosphorylation
S268 Phosphorylation

Research Backgrounds

Function:

F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity).

PTMs:

Ubiquitinated and degraded by the APC/C-Cdh1 complex.

Subcellular Location:

Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with SKP1. Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity).

Family&Domains:

The KEN box is required for the association with the APC/C-Cdh1 complex.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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