CDCA7 Antibody | Affinity Biosciences

WB
IF/ICC

1/3

Western blot analysis of extracts from Hela, using CDCA7 Antibody.

Product:	CDCA7 Antibody
Catalog:	DF3545
Description:	Rabbit polyclonal antibody to CDCA7
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.:	43 KD; 43kD(Calculated).
Uniprot:	Q9BWT1
RRID:	AB_2835918

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(82%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

CDCA7 Antibody detects endogenous levels of total CDCA7.

RRID:

AB_2835918
Cite Format: Affinity Biosciences Cat# DF3545, RRID:AB_2835918.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CDCA7; Cell division cycle associated 7; JPO1;

Immunogens

Immunogen:

Uniprot:

Q9BWT1(CDCA7_HUMAN) >>Visit HPA database.

Gene(ID):

CDCA7(83879)

Expression:

Q9BWT1 CDCA7_HUMAN:

Ubiquitous with higher level in thymus and small intestine. Overexpressed in a large number of tumors, in blood from patients with acute myelogenous leukemia (AML) and in chronic myelogenous leukemia (CML) blast crisis.

Sequence:

Q9BWT1 CDCA7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDARRVPQKDLRVKKNLKKFRYVKLISMETSSSSDDSCDSFASDNFANTRLQSVREGCRTRSQCRHSGPLRVAMKFPARSTRGATNKKAESRQPSENSVTDSNSDSEDESGMNFLEKRALNIKQNKAMLAKLMSELESFPGSFRGRHPLPGSDSQSRRPRRRTFPGVASRRNPERRARPLTRSRSRILGSLDALPMEEEEEEDKYMLVRKRKTVDGYMNEDDLPRSRRSRSSVTLPHIIRPVEEITEEELENVCSNSREKIYNRSLGSTCHQCRQKTIDTKTNCRNPDCWGVRGQFCGPCLRNRYGEEVRDALLDPNWHCPPCRGICNCSFCRQRDGRCATGVLVYLAKYHGFGNVHAYLKSLKQEFEMQA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

Bovine

Sheep

Pig

Xenopus

Dog

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BWT1 As Substrate

Q9BWT1

Site	PTM Type	Enzyme	Source
K9	Acetylation		Uniprot
K9	Ubiquitination		Uniprot
S67	Phosphorylation		Uniprot
K75	Sumoylation		Uniprot
K75	Ubiquitination		Uniprot
S80	Phosphorylation		Uniprot
T81	Phosphorylation		Uniprot
S104	Phosphorylation		Uniprot
S106	Phosphorylation		Uniprot
S110	Phosphorylation		Uniprot
K123	Ubiquitination		Uniprot
K126	Ubiquitination		Uniprot
K131	Sumoylation		Uniprot
K131	Ubiquitination		Uniprot
S142	Phosphorylation		Uniprot
S152	Phosphorylation		Uniprot
T163	Phosphorylation	P31749 (AKT1)	Uniprot
S190	Phosphorylation		Uniprot
K204	Ubiquitination		Uniprot
Y205	Phosphorylation		Uniprot
K212	Ubiquitination		Uniprot
T213	Phosphorylation		Uniprot
K276	Ubiquitination		Uniprot
K349	Ubiquitination		Uniprot
Y359	Phosphorylation		Uniprot
K361	Ubiquitination		Uniprot
K364	Sumoylation		Uniprot
K364	Ubiquitination		Uniprot

Research Backgrounds

Q9BWT1_CDCA7_HUMAN

Function:

Participates in MYC-mediated cell transformation and apoptosis; induces anchorage-independent growth and clonogenicity in lymphoblastoid cells. Insufficient to induce tumorigenicity when overexpressed but contributes to MYC-mediated tumorigenesis. May play a role as transcriptional regulator.

PTMs:

Phosphorylation at Thr-163 promotes interaction with YWHAE and YWHAZ, dissociation from MYC and sequestration in the cytoplasm. In vitro, phosphorylated at Thr-163 by AKT.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Predominantly nuclear with some expression also seen in the cytoplasm. Predominantly cytoplasmic when phosphorylated at Thr-163.

Tissue Specificity:

Subunit Structure:

Interacts with MYC (via C-terminus), YWHAE and YWHAZ.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CDCA7 Antibody - #DF3545