Product: CDK5RAP3 Antibody
Catalog: DF3547
Description: Rabbit polyclonal antibody to CDK5RAP3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Mol.Wt.: 60 KD; 57kD(Calculated).
Uniprot: Q96JB5
RRID: AB_2835920

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 100ul $280 In stock
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Product Info

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

CDK5RAP3 Antibody detects endogenous levels of total CDK5RAP3.
Cite Format: Affinity Biosciences Cat# DF3547, RRID:AB_2835920.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


C53; CDK5 activator binding protein C53; CDK5 activator-binding protein C53; CDK5 regulatory subunit associated protein 3; CDK5 regulatory subunit-associated protein 3; Cdk5rap3; CK5P3_HUMAN; HSF 27; HSF27; IC53; Ischemic heart CDK5 activator binding protein C53; LXXLL/leucine zipper containing ARFbinding protein; LZAP; MST016; OK/SW cl.114; PP1553; Protein HSF-27;



Ubiquitously expressed (PubMed:12054757, PubMed:10721722). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta (PubMed:12737517).


PTMs - Q96JB5 As Substrate

Site PTM Type Enzyme
K29 Ubiquitination
S81 Phosphorylation
T82 Phosphorylation
K83 Ubiquitination
Y104 Phosphorylation
Y110 Phosphorylation
S115 Phosphorylation
S116 Phosphorylation
K135 Ubiquitination
Y143 Phosphorylation
S144 Phosphorylation
K146 Ubiquitination
Y162 Phosphorylation
S164 Phosphorylation
K166 Ubiquitination
R233 Methylation
Y239 Phosphorylation
Y346 Phosphorylation
T347 Phosphorylation
S372 Phosphorylation
T394 Phosphorylation
K395 Ubiquitination
K397 Ubiquitination
K440 Ubiquitination
K442 Ubiquitination
K450 Sumoylation
K450 Ubiquitination
K451 Ubiquitination
K457 Ubiquitination
K471 Ubiquitination
K478 Ubiquitination
K484 Ubiquitination
K492 Ubiquitination
Y494 Phosphorylation
T504 Phosphorylation
S505 Phosphorylation

Research Backgrounds


Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation. Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation. Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint. Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation. May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress. May also play a role in the rupture of the nuclear envelope during apoptosis. May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1.

(Microbial infection) May be negatively regulated by hepatitis B virus large envelope protein mutant pre-s2 to promote mitotic entry.


May be phosphorylated by CDK5.

Ubiquitinated. Probably triggers proteasomal degradation and is negatively regulated by UFL1.

May be ufmylated.

Cleaved by caspases early during apoptosis, the resulting peptides may play a role in rupture of the nuclear envelope.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Colocalizes and associates with microtubules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.

Subunit Structure:

Interacts with CDK5R1; competes with CDK5RAP1 and CDK5RAP2. Interacts with RELA. Interacts with CHEK1; may negatively regulate CHEK1 and thereby stimulate entry into mitosis. Interacts with CDKN2A/ARF and MDM2; forms a ternary complex involved in regulation of p53/TP53. Interacts with UFL1; the interaction is direct. Interaction with UFL1 anchors CDK5RAP3 in the cytoplasm, preventing its translocation to the nucleus which allows expression of the CCND1 cyclin and progression of cells through the G1/S transition (By similarity). Interacts with DDRGK1. Interacts with MAPK14. Interacts with CCNB1. Interacts with TUBG1; may regulate CDK5RAP3 in mitotic G2/M transition checkpoint.

(Microbial infection) Interacts with hepatitis B virus large envelope protein mutant pre-s2; promotes mitotic entry.


Belongs to the CDK5RAP3 family.

Restrictive clause


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