Cytochrome P450 4Z1 Antibody | Affinity Biosciences

WB
Cites

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Western blot analysis of extracts from rat brain cells, using Cytochrome P450 4Z1 Antibody.

Product:	Cytochrome P450 4Z1 Antibody
Catalog:	DF3590
Description:	Rabbit polyclonal antibody to Cytochrome P450 4Z1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	59 KD; 59kD(Calculated).
Uniprot:	Q86W10
RRID:	AB_2835962

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Cytochrome P450 4Z1 Antibody detects endogenous levels of total Cytochrome P450 4Z1.

RRID:

AB_2835962
Cite Format: Affinity Biosciences Cat# DF3590, RRID:AB_2835962.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CP4Z1_HUMAN; CYP4A20; CYP4Z1; CYPIVZ1; Cytochrome P450 4Z1; Cytochrome P450 family 4 subfamily Z polypeptide 1;

Immunogens

Immunogen:

Uniprot:

Q86W10(CP4Z1_HUMAN) >>Visit HPA database.

Gene(ID):

CYP4Z1(199974)

Expression:

Q86W10 CP4Z1_HUMAN:

Preferentially detected in breast carcinoma tissue and mammary gland, whereas only marginal expression is found in all other tested tissues.

Sequence:

Q86W10 CP4Z1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPSWLQELMAHPFLLLILLCMSLLLFQVIRLYQRRRWMIRALHLFPAPPAHWFYGHKEFYPVKEFEVYHKLMEKYPCAVPLWVGPFTMFFSVHDPDYAKILLKRQDPKSAVSHKILESWVGRGLVTLDGSKWKKHRQIVKPGFNISILKIFITMMSESVRMMLNKWEEHIAQNSRLELFQHVSLMTLDSIMKCAFSHQGSIQLDSTLDSYLKAVFNLSKISNQRMNNFLHHNDLVFKFSSQGQIFSKFNQELHQFTEKVIQDRKESLKDKLKQDTTQKRRWDFLDILLSAKSENTKDFSEADLQAEVKTFMFAGHDTTSSAISWILYCLAKYPEHQQRCRDEIRELLGDGSSITWEHLSQMPYTTMCIKECLRLYAPVVNISRLLDKPITFPDGRSLPAGITVFINIWALHHNPYFWEDPQVFNPLRFSRENSEKIHPYAFIPFSAGLRNCIGQHFAIIECKVAVALTLLRFKLAPDHSRPPQPVRQVVLKSKNGIHVFAKKVC

PTMs - Q86W10 As Substrate

Q86W10

Site	PTM Type	Source
Y55	Phosphorylation	Uniprot
T154	Phosphorylation	Uniprot
Y376	Phosphorylation	Uniprot
K492	Ubiquitination	Uniprot

Research Backgrounds

Q86W10_CP4Z1_HUMAN

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type II membrane protein. Microsome membrane>Single-pass type II membrane protein.

Tissue Specificity:

Preferentially detected in breast carcinoma tissue and mammary gland, whereas only marginal expression is found in all other tested tissues.

Family&Domains:

Belongs to the cytochrome P450 family.

References

1). Plasma membrane localization of CYP4Z1 and CYP19A1 and the detection of anti-CYP19A1 autoantibodies in humans. International Immunopharmacology, 2019 (PubMed: 31082724) [IF=5.6]

Application: IF/ICC Species: human Sample: MCF-7 or MCF-10A cells

Fig. 2.| Detection of CYP4Z1 on the outer surface of non-permeabilized MCF-7 or MCF-10A cells by rabbit anti-CYP4Z1 IgG and goat anti-rabbit IgG conjugated to Alexa fluor 488. The non-permeabilized cells were probed with antibodies and stained with DAPI and Deep Red plasma membrane stain as indicated. Magnification is×1,000 for all images

2). Concomitant occurence of multiple autoantibodies against human cytochromes P450. International Immunopharmacology, 2021 (PubMed: 34464888) [IF=5.6]

3). Involvement of WNT2 in trophoblast cell behavior in preeclampsia development. CELL CYCLE, 2020 (PubMed: 32779546) [IF=4.3]

4). Upregulation of estrogen receptor alpha (ERα) expression in transgenic mice expressing human CYP4Z1. Breast Cancer Research and Treatment, 2021 (PubMed: 34725776) [IF=3.8]

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Cytochrome P450 4Z1 Antibody - #DF3590